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An antibody to VacA of Helicobacter pylori in cerebrospinal fluid from patients with Guillain-Barre syndrome

¹ Department of Neurology, School of Medicine, Sapporo Medical University, Sapporo, Japan
² Department of Microbiology, School of Medicine, Sapporo Medical University
³ Department of Gastroenterology, Hokkaido University Graduate School of Medicine, Sapporo
⁴ Higeta Shoyu Co Ltd, Chiba, Japan
⁵ Institute of Immunology, Tochigi, Japan

Correspondence to:
Correspondence to:
Dr Chiba, Department of Neurology, School of Medicine, Sapporo Medical University, Minami 1-Jo Nishi 16 chome, Chuo-ku, Sapporo 060-8543, Japan;
chiba{at}sapmed.ac.jp

Methods: CSF samples from 13 patients with GBS (electrophysiologically classified as eight acute inflammatory demyelinating polyradiculoneuropathy (AIDP), four acute motor axonal neuropathy (AMAN), and one unexcitable nerve conduction) and eight disease control patients were studied. The r-VacA protein was separated by SDS/PAGE, and Western blot analysis was carried out.

Results: Six of the 13 patients with GBS had a specific IgG antibody to VacA of H pylori, which was confirmed by absorption experiments using r-VacA. Every patient with positive CSF anti-r-VacA IgG had AIDP.

Conclusion: The sequence homology previously found between VacA and human (Na⁺+K⁺)-ATPase A subunit suggests that antibodies to VacA involve ion channels in abaxonal Schwann cell plasmalemma resulting in demyelination in some patients with GBS.

Keywords: Helicobacter pylori; VacA antibody; cerebrospinal fluid; Guillain-Barre syndrome

Abbreviations: GBS, Guillain-Barre syndrome; AIDP, acute inflammatory demyelinating polyradiculoneuropathy; AMAN, acute motor axonal neuropathy; CSF, cerebrospinal fluid; VacA, vacuolating cytotoxin

This article has been cited by other articles:

				S. Chiba, T. Sugiyama, K. Yonekura, S. Tanaka, H. Matsumoto, N. Fujii, S. Yokota, and T. Hirayama An antibody to VacA of Helicobacter pylori in the CSF of patients with Miller-Fisher syndrome Neurology, December 14, 2004; 63(11): 2184 - 2186. [Abstract] [Full Text] [PDF]