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Discrepancies between cerebral perfusion and metabolism after subarachnoid haemorrhage: a magnetic resonance approach
  1. Jeremy Rowea,b,
  2. Andrew M Blamirea,
  3. Zayne Domingoa,
  4. Virginia Moodyc,
  5. Andrew Molyneuxc,
  6. James Byrnec,
  7. Thomas Cadoux-Hudsona,b,
  8. George Raddaa
  1. aMRC Magnetic Resonance Spectroscopy Unit, John Radcliffe Hospital, Oxford, UK, bDepartment of Neurosurgery, cDepartment of Neuroradiology, Radcliffe Infirmary, Oxford, UK
  1. Dr Jeremy Rowe, Department of Neurosurgery, Radcliffe Infirmary, Woodstock Road, Oxford OX2 6HE, UK. Telephone 0044 1865 311188; fax 0044 1865 224898.


INTRODUCTION There is a variable relation between angiographic vasospasm and delayed ischaemic neurological deficit (DIND). Magnetic resonance (MR) techniques have the potential to investigate the haemodynamic, metabolic, and structural changes occurring with these complications. These techniques have been applied to study DIND in patients recovering from subarachnoid haemorrhage.

METHODS Fifteen studies were performed on 11 patients, 10 with DIND. Vasospasm was diagnosed angiographically or with transcranial Doppler. The MR protocol consisted of T2 weighted imaging, contrast enhanced dynamic perfusion scanning, TI weighted imaging, and two dimensional localised proton spectroscopy. Relative cerebral blood volume maps were generated from perfusion scans. Metabolite ratios were calculated from proton spectra.

RESULTS All patients had cortical oedema on T2 weighted images, significantly more pronounced in patients of poor clinical grade (p<0.01). Spectra were normal in good grade patients. Lactate was increased and N-acetyl aspartate decreased in the poor grades, significantly worse in grade 4 compared with grade 3 patients (p<0.05). Spectral changes also correlated with the severity of oedema (p<0.05). Relative blood volumes were significantly higher in oedematous regions of poor compared with good grade patients (p<0.05). Lactate was seen in regions of the brain with increased relative blood volume.

CONCLUSIONS Despite the paramagnetic effects of haemorrhage, or of the coils and clips used to treat aneurysms, this study demonstrates that patients recovering from subarachnoid haemorrhage can undergo complex MR studies. Oedema, lactate, and increased relative blood volume correlate well with each other and with DIND and poor clinical grade.

  • subarachnoid haemorrhage
  • vasospasm
  • magnetic resonance
  • delayed ischaemic neurological deficit

Statistics from

Vasospasm, which is associated with delayed ischaemic neurological deficits (DIND), is regarded as the leading cause of death and disability in patients surviving the initial ictus of subarachnoid haemorrhage.1 The relation, however, of angiographic vasospasm with DIND is variable, vasospasm sometimes being found in asymptomatic patients.2 Cerebral blood flow has been studied using 133Xe, positron emission tomography (PET), and single photon emission tomography (SPECT) in patients with vasospasm after subarachnoid haemorrhage.3 Whereas most of these studies have shown that cerebral blood flow is reduced during the period of angiographic vasospasm, these results have also been variable.3 In addition raised cerebral blood volumes have been reported in poor grade patients, suggesting a compensatory dilatation of the capillary bed in response to the proximal vasospasm.4 PET studies have also suggested the existence of an additional toxic or metabolic abnormality as the reduction in oxygen consumption is not explained by the decrease in blood flow.5-9

In an attempt to explore the pathophysiology of DIND, an integrated magnetic resonance (MR) protocol was used to examine the structural, metabolic, and haemodynamic changes which occur. In addition, given concerns that haemorrhage, and the coils and clips used to treat aneurysms, could have paramagnetic effects, degrading the quality of the MR data, the feasability of acquiring this information in a selected neurologically stable group of patients recovering from subarachnoid haemorrhage was assessed, this work being undertaken as a pilot study.

Clinical material and methods


Fifteen studies were performed on 11 selected patients with aneurysmal subarachnoid haemorrhage (table 1). Their mean age was 49 (range 31–63) years, and there were 10 women and one man in this group. Patients were selected because they had DIND but were stable, or in one case because of the risk of developing DIND due to extensive subarachnoid blood. The mean time of first study was day 11, range 3–26 (day 0 being the day of the presenting haemorrhage). The range reflects constraints of the patients’ clinical stability and the availability of scanning time. All patients were assessed neurologically by a neurosurgeon and graded according to the Hunt and Hess scale10 on the day of the MR study. The initial diagnostic CT was used by a neurosurgeon to grade the extent of subarachnoid blood, and reflecting the selection of patients with DIND, 10 of the 11 patients were Fisher grade 3 or 4.11 Patients developing DIND all routinely had CT to exclude further haemorrhage or hydrocephalus as a cause for their deterioration. The diagnosis and distribution of vasospasm was assessed independently and blindly by consultant neuroradiologists from angiograms obtained for clinical reasons. Transcranial Doppler was not used in all patients reflecting consultant preference, but when applied a velocity exceeding 120 cm/s was taken as diagnostic for middle cerebral artery and 150 cm/s for anterior cerebral artery vasospasm.3 With one exception, all patients were studied after the aneurysm had been secured. In seven patients this was by an endovascular technique using Guglielmi detachable coils, and in three patients by conventional surgery. All patients received nimodipine and once the aneurysm had been secured, symptomatic patients were treated with volume expansion and inotropes. Informed consent was obtained before doing the study and the research protocol was approved by the Central Oxford research ethics committee.

Table 1

Patient details (clinical assessment used the Hunt and Hess grading system; Glasgow coma scores (GCS) were not assigned when focal neurology was causing verbal impairment)


Studies used a 1.5 T Siemens Magnetom Vision MR scanner. Initially standard T2 weighted images (turbo spin echo sequence, TE 96 ms, TR 3976 ms) were acquired, and examined for areas of signal change which defined regions of interest and guided the rest of the examination. Dynamic perfusion scanning was performed with a single T2 weighted 10 mm thick slice placed through the region of interest, using a spin echo prepared turbo-FLASH sequence (TE 80 ms, TR 8.5 ms, flip angle 10°) to collect 80 images, with a repetition rate of 1.2 seconds. Fifteen millilitres of 0.5 mM gadolinium DTPA were given intravenously, starting on the 10th image, through a 20 gauge antecubital line, or a central line if present. T1 weighted images (spin echo sequence, TE 14 ms, TR 651 ms) were then acquired to assess parenchymal enhancement with gadolinium, indicative of a breakdown of the blood brain barrier. Localised proton spectroscopy was performed with a PRESS spectroscopic imaging technique.12 The PRESS sequence (TE 135 ms, TR 1.5 s) selected signal from a 50×50×15 mm3 volume extending from the region of interest into the surrounding brain. Signal from within this volume was further localised using spectroscopic imaging (16×16 phase encodes) yielding nominal voxels of 10×10×15 mm3. The region of interest was selected to include the oedematous area on the T2 weighted image.

The total mean time in the magnet for patients completing the protocol was 42 (SD 6) minutes. This was achieved by using the standard clinical imaging sequences and limiting the spectroscopy preparation time by utilising the autoshim. The time taken for each stage of the protocol was as follows: scouts 30 seconds, T2 weighted MRI two minutes, DPS preparation 36 seconds, DPS acquisition 96 seconds, T1 weighted MRI four minutes, MRS preparation about 10 minutes, MRS acquisition 13 minutes. Non-invasive blood pressure, pulse oximetry, and electrocardiographic monitoring were available for all patients.


The T1 and T2 weighted images were reported blindly and independently by consultant neuroradiologists. Signal changes suggestive of oedema and parenchymal enhancement with gadolinium were graded as absent (0), moderate (1), or pronounced (2).

Spectroscopy data were analysed using the Siemen’s software which displayed spectra from 50×50×15 mm3 slice as contiguous 10×10 mm2 voxels. Spectral peaks from creatine (Cr), choline (Ch), N-acetyl aspartate (NAA), and lactate if present, were fitted to Lorentzian line shapes and integrated. Line widths were used to assess paramagnetic effects. The combined value of the creatine and choline integrals (Cr+Ch) was used as a reference and the other metabolite integrals expressed as ratios relative to this in addition to using Cr/Ch as a constant. Where adjacent voxels showed the same qualitative changes, the ratios were averaged together to give a mean value for that region. The metabolite ratios were correlated with the qualitative changes on the T2 weighted image, and changes in the perfusion scan.

The image data generated from the DPS was exported and analysed off line using purpose written software. The change in the signal intensity due to the passage of gadolinium was converted into a relative gadolinium concentration-time curve, the integral of which was used to calculate a relative cerebral blood volume map.13 14 Changes in relative blood volume were expressed as a percentage of a symmetrically placed region of interest in the contralateral hemisphere which had a normal MR appearance. Diffuse vascular changes after subarachnoid haemorrhage affecting both hemispheres may not be detected by this method. To verify the significance of any asymmetry found, a multistage process described in the was applied. In addition, the time course of the signal intensity changes were plotted to follow the passage of gadolinium through the brain, in those selected regions of interest.

Statistical comparisons used Student’s t test. Hunt and Hess clinical grades were compared with the severity of oedema, metabolite ratios, and changes in relative blood volume in that region. Blood volumes from distinct regions corresponding to spectroscopy voxels were used to correlate the results of the perfusion scan with the metabolite ratios. Values are quoted as mean (SD).


Table 2 shows the results for the individual patients, including details of the relevant clinical investigations performed. Vasospasm was confirmed in eight patients angiographically or with transcranial Doppler within a mean of two days from the first MR study. In general the pattern of vasospasm reflected the site of the aneurysm, the location and extent of subarachnoid blood detected by CT, and concurred with the distribution of focal neurological deficits found clinically. Of the three patients without vasospasm, one (case 2) was studied on day 3, when she was asymptomatic and before it might have developed. A second patient (case 10) deteriorated neurologically eight days after her presenting haemorrhage, developing at that time areas of radiolucency on CT. The MR study was performed late (day 26), once her clinical condition had stabilised and the appearance of her CT resolved. The third patient (case 9), despite developing DIND nine days after the presenting subarachnoid haemorrhage, had no evidence of lumenal narrowing on concurrent angiography, and no other causative factor could be identified to account for her neurological deterioration.

Table 2

Patient investigations


Figure 1 shows typical images acquired in this study. All 11 patients had evidence of oedema on the T2 weighted scans, with increased signal intensity and effacement of the sulci. These changes were localised, were most obvious cortically, and their distribution was in keeping with the presence of focal neurological deficits. Pronounced changes of oedema were significantly more common in poor grade (3 and 4) compared with good grade patients (p<0.01). Breakdown of the blood brain barrier resulting in parenchymal enhancement with gadolinium was seen in four patients, and occurred in areas where pronounced oedema had been noted on the T2 weighted scans. It seemed to be a delayed phenomenon.

Figure 1

Typical MRI changes. Left: T2 weighted image showing oedema with sulcal effacement and high signal in the left middle cerebral artery territory. Centre: T1 weighted image after gadolinium, without parenchymal enhancement. Right: parenchymal enhancement with gadolinium was seen, but only late, a minimum of 14 days after subarachnoid haemorrhage.


Spectra from good grade patients were normal with no lactate present (fig 2 A). Small lactate peaks were detected in three of the four initial studies carried out on grade 3 patients (fig 2 B). In the two grade 4 patients studied, both had a considerable decrease in NAA, and lactate was seen in one. In all patients, the average metabolite ratios from the most severely affected ROI correlated with the clinical grade (table 3 A). The NAA/(Cr+Ch) ratio was significantly lower in grade 4 compared with grade 3 patients (p<0.05), and grade 3 patients tended to have lower values than grades 1 and 2. Conversely, the lactate/(Cr+Ch) ratio increased with worsening clinical grade. Metabolite ratios were also related to the severity of oedema on the T2 weighted images (table 3 B). Spectra from the most swollen areas of the brain had significantly lower NAA/(Cr+Ch) ratios than spectra from moderately swollen or unaffected areas (p<0.05). The lactate/(Cr+Ch) ratio also tended to be higher where the oedema was most pronounced, although this did not reach significance. Changes in metabolite ratios seemed to be due to altered NAA and lactate concentrations as there were no significant changes in the Cr/Ch ratio with changes in clinical grade or severity of oedema.

Figure 2

Illustrative spectra (A) from a good grade patient with normal metabolite ratios and (B) from a poor grade patient showing reduced NAA and a lactate peak.

Table 3

Mean (SD) values for the metabolite ratios, and for the percentage asymmetry in relative cerebral blood volume related to (A) the Hunt and Hess clinical grade, (B) to the degree of oedema assessed on the T2 weighted image, and (C) individual values generated from follow up studies

Repeat spectroscopy studies were performed in three patients. NAA/(Ch+Cr) improved in these patients, and the lactate peaks resolved (table 3 C). These changes accompanied clinical improvement.


Figure 3 shows the time course of the signal changes due to the passage of gadolinium, and the cerebral blood volume map calculated from this data. Within the sensitivity of this measurement there is no difference in the temporal characteristics between the affected and contralateral hemisphere. Blood volume changes, relative to symmetric regions of interest in the contralateral hemisphere, were variable. However, if only the most swollen region of the brain defined from the T2 weighted image was considered, blood volume increased as clinical grade worsened (table 3 A). Relative blood volumes were significantly higher in grade 3 and 4 compared with grade 1 and 2 patients (p<0.05) and the highest blood volumes were seen in grade 4 patients.

Figure 3

The relation of perfusion changes to cortical oedema. Left: T2 weighted image with oedema in the right middle cerebral artery territory. Right: increased relative blood volume (high signal) mapping to the oedematous region. Below: the time course of the signal intensity changes for the swollen high volume region (solid line), and for a symmetric region from the left (normal) hemisphere (broken line). The difference in relative blood volume is generated by the difference in amplitude, whereas the similar time course suggests that perfusion is greater in the swollen brain than in the contralateral asymptomatic side. Despite this lactate was seen in these areas of high relative cerebral blood volume.  

The finding of both raised relative blood volume and lactate in regions of cerebral oedema in poor grade patients, means that lactate is associated with areas of high relative blood volume.


There have been few MR studies of subarachnoid haemorrhage, either with conventional imaging or with spectroscopy. The advantage of a combined approach is that it provides structural, metabolic, and haemodynamic information as a single study, and so offers a potent way of exploring physiological and pathological processes. When applied to the DIND which may complicate subarachnoid haemorrhage, there are discrepancies between cerebral perfusion and metabolism, suggesting that symptomatic changes seen in DIND may not simply be the result of a reduction in blood flow.

Previous imaging studies have considered the feasibility, safety, and diagnostic use of MRI in acute subarachnoid haemorrhage.15-17 Although these series have commented on the sensitivity of MRI for detecting oedema, such changes have not been detected in preoperative studies. Postoperative changes, either at the site of the craniotomy possibly due to local trauma,15 or remote from it in the deep tissues16 were described. In keeping with this similar changes were seen in the three surgically treated patients in this series. Whereas in these cases the cortical signal changes could be due to surgical trauma, similar changes were also found in the seven patients treated endovascularly and in the patient with an untreated aneurysm. Furthermore, the anatomical distribution of these changes were in keeping with the patients’ focal neurological deficits, and the severity of the changes were significantly related to clinical grade. These changes may not have been noted in earlier studies because these have examined predominantly good grade patients, by contrast with the grade 3 and 4 cases in this series. The predominanently cortical distribution of these signal changes was striking, which may reflect the higher metabolic rate of grey compared with white matter, or the importance of exposure to subarachnoid blood clot and its degradation products.

Although MRS studies of subarachnoid haemorrhage are limited, the metabolic changes of cerebral ischaemia have been well documented both in animal models and in human stroke with proton and phosphorus spectroscopy.18 19 With ischaemia and infarction, lactate accumulates and NAA is lost. Whereas acutely the lactate may reflect anaerobic metabolism, it can persist for weeks or even months after the stroke,20 when its presence has been attributed to macrophage invasion and gliosis.21 NAA is regarded as a neuronal marker, and decreases have been interpreted as a loss of neurons.

Phosphorus spectroscopy after subarachnoid haemorrhage has detected acidosis and a loss of high energy phosphates in symptomatic patients, these changes resolving with neurological recovery.22 23Similar changes have been reported in a primate model.24The finding in this study of increased lactate and decreased NAA is therefore not surprising. The relation of the spectral changes to the clinical grade of the patients, to the severity of the oedema, and the improvement in metabolite ratios seen as patients recovered, illustrate the close association of metabolism with cellular function and the accumulation of water.18 25

In this study a significant increase in the relative blood volume was seen in the most swollen regions of the brain of patients in the poorest clinical grades. Grubb et al, in an early study using fixed iodide counters, reported a 58% increase in blood volume in 14 examinations of poor grade patients with angiographic lumenal narrowing, but variable changes in a further 31 examinations of patients without vasospasm or in better grades.4Subsequent PET studies have confirmed the finding of increased blood volume,7 8 or have reported no change.5 6This increase in blood volume in poor grade patients may represent dilatation of the intraparenchymal vessels compensating for proximal vasospasm.4

This study has shown the practicality of using an integrated MR protocol in patients with subarachnoid haemorrhage to investigate structure, metabolism, and cerebral blood volume. The results have confirmed a close relation between lactate, oedema, and clinical grade. In keeping with previous studies, increased relative cerebral blood volume has also been associated with these changes. Reflecting these associations lactate is found in areas of high relative cerebral blood volume. The understanding of this relation and the determination of parenchymal perfusion and metabolism in these areas may alter the approach to patient management.


To test whether asymmetries in relative blood volume measurements were significant, the following process was applied:

(1)The relative gadolinium concentration-time curve [C(t)] for the region of interest, and for a symmetric region of interest from the contralateral hemisphere were determined. Relative blood volume is the integral of this curve.

(2) One curve was subtracted from the other to generate a relative concentration difference - time course [ΔC(t)].

(3) The variance [ς 2] in this concentration difference-time course due to noise was calculated from the points before the signal change due to the gadolinium.

(4) The time course of the z score [z(t)] was generated by dividing the concentration differences by the standard deviation calculated from the noise level:

z(t) = ΔC(t) / ς

(5) The concentration-time curves were then defined as differing significantly when z(t) averaged over three consecutive points exceeded 1.96 (p<0.05) during the passage of the gadolinium.


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