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Pattern of dopaminergic loss in the striatum of humans with MPTP induced parkinsonism
  1. Barry J Snowa,
  2. Francois J G Vingerhoetsa,
  3. J William Langstonc,
  4. James W Tetrudc,
  5. Vesna Sossib,
  6. Donald B Calnea
  1. aThe Neurodegenerative Disorders Centre, University of British Columbia, Vancouver, BC, Canada, bUniversity of British Columbia, TRIUMF, Vancouver, BC, Canada, cThe Parkinson's Institute, 1170 Morse Avenue, Sunnyvale, California, USA
  1. Dr Barry J Snow, Department of Neurology, Auckland Hospital, Private Bag 92024, Auckland, New Zealand. Telephone 0064 25 314439; fax 0064 9 307 4924; email: bsnow{at}ahsl.co.nz

Abstract

OBJECTIVES To examine the distribution of striatal dopaminergic function in humans with parkinsonism induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to determine if there is a caudate-putamen gradient as is seen in idiopathic Parkinson's disease.

METHODS We scanned nine humans exposed to MPTP with parkinsonism ranging from minimal to severe using [18F]fluorodopa (FD) and high resolution PET. The results were compared with those of 10 patients with Parkinson's disease and six normal subjects.

RESULTS In the MPTP group there was an equal degree of reduction of dopaminergic function in the caudate and putamen. This was different from the greater putaminal than caudate loss in Parkinson's disease (p<0.001).

CONCLUSIONS Parkinson's disease is not caused by transient exposure to MPTP.

  • 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)
  • Parkinson's disease
  • positron emission tomography
  • fluorodopa

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1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) produces parkinsonism in both humans and animals.1 2 Clinically, the syndrome may be almost indistinguishable with bradykinesia, rigidity, impaired postural reflexes, sometimes tremor, and a neuropsychological deficit similar to Parkinson's disease.3 In some humans with parkinsonism induced by MPTP, the clinical deficit and the dopaminergic deficit as disclosed by PET, is also progressive over time.4 Aged monkeys treated chronically with MPTP have been reported to have inclusions in nigral dopaminergic neurons that are reminiscent of Lewy bodies.5

A characteristic feature of Parkinson's disease is the striatal gradient of loss of indices of striatal dopaminergic function, with the greatest deficit being in the putamen.6 Experiments with MPTP in non-human primates have found different patterns of striatal dopamine deficit.7-13 Some studies have found the loss of caudate dopamine to be equal or greater than the dopamine loss from the putamen, and it has been suggested that this finding indicates that a substance similar to MPTP is unlikely to be the underlying cause of Parkinson's disease.8 By contrast with those findings, other workers have produced a caudate putamen gradient similar to that in Parkinson's disease using low doses or chronic administration of MPTP to primates.10 12 13 There is a similar variation in findings of the patterns of cell and dopamine loss in the substantia nigra.5 14 15 The postmortem distribution of the striatal dopaminergic deficit in human MPTP parkinsonism has not yet been described.

The regional variation of striatal dopamine loss in Parkinson's disease is clearly shown with [18F]-fluorodopa (FD) PET using high resolution tomographs.16 17 Our previous reports of FD PET in human parkinsonism induced by MPTP described scans performed on a PETT VI scanner with a resolution insufficient to discriminate caudate from putamen.4 18 We now present the results of FD PET of human MPTP parkinsonism on a high resolution scanner. We used these data to examine the pattern of the striatal dopaminergic loss in MPTP parkinsonism.

Methods

All assessments were performed at the University of British Columbia. Nine subjects were studied about 10 years after having been exposed to MPTP. Eight had mild parkinsonism (mean age 39.4, range 30–55, men/women 4/4, mean modified Columbia score (MCS19) while off medication 6.4, range 2–16). Detailed descriptions of their clinical status and total striatal PET results have been presented recently.4 In addition, a severely affected woman aged 34 years with Hoehn and Yahr20 stage V parkinsonism and an MCS of 70 was scanned.

Two control groups were scanned. The first consisted of 10 patients with Parkinson's disease (mean age 53.6, range 33–74; four men, six women; mean MCS 22.9, range 4-44). The second consisted of six normal subjects (mean age 66.0, range 52–80; twomen, four women).

This study was performed on patients who were being rescanned on a PETT VI scanner as part of our longitudinal study reported previously.4 That scanner does not have sufficient resolution to examine the putamen/caudate ratio. We therefore devised a protocol where we followed the PETT VI scan with a further scan on a high resolution scanner. By 2 hours after injection 18F has decayed for more than one half life. To deal with the reduced signal we scanned on an ECAT 953–31B scanner in 3D mode.21 This increases the number of recorded true coincidences by a factor of six, which more than compensates for the effect of radioactive decay. The ECAT scanner has a resolution of 5.6×5.6×5 mm, full width at half maximum. Each image has 31 slices with a centre-centre separation of 3.375 mm. Each subject was given 100 mg carbidopa orally 1 hour before scanning. Before the PETT VI scan, the subjects were positioned in the ECAT camera using gantry mounted lasers so that the orbitomeatal line was parallel with the detector rings. An individually moulded thermoplastic face mask was then fitted to restrain head movement and to permit respositioning. Marks were made on the subject's face mask, corresponding to the laser lines, to assist repositioning for the emission scan. A transmission scan was performed using rotating rod sources. The subject was then transferred to the PETT VI camera for a 120 minute dynamic FD scan (dose of FD 2.5–3 mCi, injected while the subject was in the PETT VI scanner). At the end of the period on the PETT VI scanner, the subjects were returned to the ECAT scanner and scanned for 15 minutes with the septae removed (3D mode22). The 3D emission data were corrected for scatter using the algorithm of Bailey et al.23 The images were then reconstructed as a single image of 31 planes using the algorithm of Kinahanet al as implemented by Townsendet al.21 Regions of interest (ROIs) 8.8 mm in diameter were placed on each image—one ROI on each caudate and three ROIs distributed along each putamen.24The ROIs were placed manually on the five slices where the striata were best seen. The mean bilateral caudate and putamen values were then calculated and expressed as a ratio of putamen/caudate.

Results

All results are from the ECAT scanner. The images from the subjects with Parkinson's disease showed marked putaminal loss of signal with a putamen/caudate ratio of 0.68 (SD 0.053) (figs 1 and 2). By contrast, the normal subjects had a homogeneous distribution of striatal radioactivity with a putamen/caudate ratio of 1.02 (SD 0.053). The MPTP parkinsonian subjects had varying degrees of loss of striatal signal with the most severe depletion in the clinically most affected patient (fig 1). The loss was always uniform; the putamen/caudate ratio was 1.01 (SD 0.050). The severely affected patient had a ratio of 0.98. The putamen/caudate ratios in the Parkinson's disease group were significantly different from the MPTP exposed (p=1.9 E 10, unpaired, two tailed t test on the log of the ratios) and normal groups (p=2.03 E 8). There was no difference between the putamen/caudate ratios of the MPTP exposed and normal groups. In the MPTP exposed group, there was no significant correlation between the putamen/caudate ratio and the overall severity of the dopaminergic lesion, the MCS, or with the age of the subject. The asymmetry of the ratios expressed as a percentage of the subject's uptake varied from 0–14% (mean 5%) in the MPTP exposed group. This was significantly less than the asymmetry in the Parkinson's disease group, which varied from 2%-23% (mean 12%, p=0.016).

Figure 1

Representative FD PET images of a normal subject (top left), patient with Parkinson's disease (top right), a mildly affected MPTP exposed subject (bottom left), and the most severely affected MPTP exposed subject (bottom right). The greater degree of putaminal loss is seen in the patient with Parkinson's disease, whereas the MPTP exposed subjects have homogeneous losses of striatal signal. The colour scale represents radioactivity concentration.

Figure 2

Scatterplots of the putamen/caudate ratio for the subject groups; • indicates the most severely affected MPTP exposed subject. The MPTP exposed group was significantly different from the control subjects (p=1.9E 10) but no different from the normal group.

The mean age and mean Columbia scores of the Parkinson's disease group were greater than those of the MPTP exposed group. Within the Parkinson's disease group, however, there was no correlation between the putamen/caudate ratios and either the age or the Columbia score.

Discussion

We have shown that in human MPTP induced parkinsonism there is an equal loss of dopaminergic function from the caudate and putamen. This contrasts with the greater putaminal loss seen in Parkinson's disease both in this study and other reports.6 16 17 Other forms of parkinsonism are associated with a uniform loss of striatal signal on FD PET. In particular, in progressive supranuclear palsy there is equal loss of caudate and putaminal signal, and progressive supranuclear palsy may be distinguished from Parkinson's disease with a high degree of certainty on the basis of the pattern of striatal FD uptake.17 25 Cortical basal ganglionic degeneration and post-traumatic parkinsonism also have a uniform loss of striatal signal in PET studies.26 27 Multiple system atrophy has a variable pattern with both uniform loss and greater putaminal loss found with PET and pathological studies.25 28

In non-human primates, acute administration of high doses of MPTP produces uniform striatal dopaminergic loss.12 By contrast, several studies have demonstrated that either a single low dose (for example, 1.5 mg/kg subcutaneously in the squirrel monkey) or chronic low doses of MPTP consistently produce a greater depletion of dopaminergic markers in the putamen than the caudate,13 29 a pattern similar to that found in Parkinson's disease. Such a pattern was not seen in any of the subjects with MPTP induced parkinsonism in this study. Furthermore, the putamen/caudate ratio did not correlate with either the overall severity of the striatal dopaminergic lesion or the clinical deficit. Two of our subjects reported that they injected drugs that were likely to contain MPTP over 3 to 4 months, a dosing schedule similar to that administered to the monkeys, yet PET demonstrated equal caudate and putaminal loss. It is possible that one or more of the injections of MPTP administered by these subjects was a high enough dose to cause a uniform caudate and putamen dopaminergic depletion. We also found that the MPTP exposed subjects had striatal dopaminergic function that was significantly more symmetric than the subjects with Parkinson's disease. Parkinson's disease typically presents asymmetrically, and this is reflected in the striatal fluorodopa uptake.

It might be argued that all of our patients were exposed to the equivalent of the high doses of MPTP that produce an equal loss of caudate and putamen dopaminergic function in animal studies. However, if this were the case, we might have expected the subjects to manifest clear cut signs of parkinsonism, yet three of our subjects were initially clinically normal and gave no history of transient parkinsonism. None the less, a feature of the animal work with MPTP is the variation in degree of dopaminergic deficit that develops in response to similar doses of the toxin. It therefore remains possible that humans might develop a striatal dopaminergic deficit with a distribution similar to Parkinson's disease if they were exposed to MPTP under different circumstances.

A further factor that needs to be considered is the effect of the subject's age on the pattern of striatal dopaminergic loss after exposure to MPTP. The mean age of our subjects was younger than the Parkinson's disease group, and it could be argued that the projection to the caudate is more vulnerable in younger subjects. Against this argument was the lack of any correlation between age and the putamen/caudate ratio in the MPTP exposed group and the fact that there was an overlap in the ages of the MPTP exposed and Parkinson's disease groups.

It is not known why there is a differential vulnerability of nigral neurons to the pathogenic process that causes Parkinson's disease or to MPTP in some non-human primate experiments.30 There are regional differences within the nigra including variations in the concentrations of neuromelanin, calbindin, and fibroblast growth factor.31-35 There are also differences between the caudate and putamen in the concentrations of dopamine reuptake sites in the striatal nerve terminals of nigral neurons.36 The relevance of these findings to the neuronal loss in Parkinson's disease is not clear.37

In conclusion, the subjects of our study have a pattern of striatal dopaminergic function that differentiates them from subjects with Parkinson's disease. This finding indicates that Parkinson's disease is not caused by transient MPTP exposure.

Acknowledgments

We thank the UBC/TRIUMF PET group, who helped with the PET scanning. This study was supported by the Medical Research Council of Canada, the National Parkinson Foundation, the Parkinson Foundation of Canada, the Movement Disorder Institute, Vancouver, and the Parkinson's Institute, Sunnyvale California.

References

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