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Diffusion tensor imaging demonstrates deviation of fibres in normal appearing white matter adjacent to a brain tumour
  1. U C Wieshmanna,
  2. M R Symmsa,
  3. G J M Parkerb,
  4. C A Clarkb,
  5. L Lemieuxa,
  6. G J Barkerb,
  7. S D Shorvona
  1. aEpilepsy Research Group, Department of Clinical Neurology, Institute of Neurology, University College London, Queen Square, London WC1N3BG, UK, bNMR Research Unit
  1. Dr U Wieshmann, National Society for Epilepsy, MRI Unit, Chalfont St Peter, Gerrards Cross, Bucks SL9 ORJ, UK emailuwiesh{at}ion.ucl.ac.uk

Abstract

the objective was to study fibre orientation in the cerebral white matter of a patient with a brain tumour using diffusion tensor imaging (DTI).

 A patient with a mild left hemiparesis and a tumour in the right frontal lobe and 20 healthy volunteers were scanned with a DTI sequence. The scans were spatially normalised and the fibre orientation in the patient compared with the fibre orientation in normal controls.

 DTI disclosed a change of the orientation of fibres in the patient compared with normal controls. In the normal appearing white matter adjacent to the tumour fibres deviated from the normal superior inferior orientation in the corona radiata by about 30o. This finding was consistent with a displacement by distant mass effect rather than a destruction of fibres, in agreement with the neurological examination.

 In conclusion, DTI demonstrated a deviation of fibres in normal appearing white matter adjacent to a tumour. The technique will improve understanding of the effects of structural abnormalities on fibres. This will assist the interpretation of clinical findings and functional imaging studies and guide neurosurgical interventions.

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In the nervous system, myelinated tracts connect different brain regions together, and the brain with the spinal cord. Studying connections in normal and abnormal brains plays an important part in our understanding of brain function. Standard magnetic resonance1 and FLAIR2 imaging techniques have been used to demonstrate tracts. However, information on the orientation of fibres within the tract is unobtainable with these methods. Diffusion imaging now provides an opportunity to study fibre orientation in vivo. In the white matter of the brain diffusion is directional (anisotropic) because water molecules diffuse predominantly parallel to tracts.3 Diffusion tensor imaging (DTI), a new MRI technique, provides quantitative measures of the molecular motion of water in a three dimensional space4 and can be used for accurate studies of fibre orientation in vivo.5 The principal eigenvector represents the principal direction of diffusion corresponding to the fibre tract axis. We used DTI in combination with spatial normalisation and a multiplanar representation of the principal eigenvector to demonstrate the directional organisation of human white matter in vivo (“tractography”). In this study we have investigated the fibre orientation in normal appearing white matter adjacent to a tumour.

Methods

We studied a 47 year old right handed man who developed partial epilepsy at the age of 38. On examination, he had only a mild paresis affecting the left leg (power 4/5) and impaired fine finger movements of the left side. Standard MRI with T1 weighted and T2 weighted sequences showed a large tumour in the right frontal lobe extending to the level of the corpus callosum. The long history of epilepsy and the appearance of the tumour on standard MRI were compatible with a low grade glioma.

DIFFUSION TENSOR IMAGING VARIABLES

We scanned the patient and 20 healthy volunteers (mean age 30 years) with a 1.5T GE scanner (GE, Milwaukee, USA, maximum gradient strength=22 mT/m, slew rate=120 T/m/s). Diffusion tensor imaging was performed using an inversion recovery prepared single shot echo planar diffusion weighted imaging sequence (TR=5000 ms, TE=78 ms, TI=1835 ms, FOV 24 cm, acquisition matrix 96×96, reconstruction matrix 128×128, 5 mm slice thickness, 2 b values applied in seven non-colinear directions at 28 slice positions (bmax=703 s/mm2)). From the measured signal on T2 weighted and diffusion weighted images, and the b matrix calculated from the pulse sequence parameters, we estimated the effective diffusion tensor in each voxel.6 The diffusion tensor matrix was diagonalised to give the three eigenvalues and their associated eigenvectors. From this information the fractional anisotropy index and the mean diffusivity were derived on a pixel by pixel basis. The fractional anisotropy index is a scalar index of diffusion anisotropy which scales from 0 (isotropic medium) to 1 (maximum anisotropy) but does not provide information on the directions of diffusion. The eigenvector corresponding to the largest of the three eigenvalues is the principal eigenvector and represents the principal direction of diffusion at that point. The mean diffusivity is a measure of the magnitude of diffusion in mm2/s averaged over all directions. For spatial normalisation the non-diffusion weighted images were registered using a linear registration technique7 to a template which consisted of one of the controls in standard Talairach's space.8 The registration parameters were then used to transform the diffusion maps. As the diffusion eigenvectors are referenced with respect to the magnet and gradient axes, it was then necessary to apply the rotation part of the registration matrix to the eigenvectors to rotate them into the common space. We displayed both principal eigenvector and anisotropy9 and compared the appearance of fibres in the patient with the appearance of fibres in controls in corresponding anatomical positions. The pyramidal tract was defined as the fibres passing trough the posterior limb of the internal capsule. The angle between the AC-PC line and the pyramidal tract was measured on sagittal images.

Fibre orientation in tumour and normal brain demonstrated with DTI. Top left: axial FLAIR images of the patient showing a tumour in the right central region. Top right: map of the mean diffusivity of the patient showing increased diffusion in the tumour. Bottom: map of the fractional anisotropy overlayed with the principal eigenvector (red bars) of the patient (left) and a control subject in the same position (right). In the normal appearing white matter adjacent the tumour the main fibre orientation (indicated by yellow bars) in the patient differs from the orientation in the brain of the control subject.

Results

The tumour was located in Talairach's sector E and F in the expected position of the pyramidal tract.8 In the tumour the mean diffusivity was increased and the fractional anisotropy was reduced. (Mean diffusivity in white matter of normal control subjects: mean=0.75×10-3 mm2/s, SD=0.05×10-3 mm2/s, in the patient= 0.91×10-3 mm2/s, fractional anisotropy in control subjects: mean=0.76, SD=0.05, in the patient: 0.29.) The abnormally high mean diffusivity values extended into the posterior limb of the internal capsule.

In the normal appearing white matter adjacent to the tumour (normal on visual inspection of T1 and T2 weighted images and within the normal range of mean diffusivity and fractional anisotropy) the orientation of the principal eigenvector differed from the orientation in corresponding regions on the contralateral side in the patient and from the orientation in the control subjects. In control subjects the angle between the AC-PC line and the pyramidal tract ranged from 50°–80°, which was in keeping which other studies.8In the patient the angle was about 35°, which implies a deviation of the principal eigenvector of about to 30° (figure).

Discussion

We demonstrated an abnormal orientation of the principal eigenvector in normal appearing white matter adjacent to the tumour. The fact that mean diffusivity and fractional anisotropy were within normal limits in this region indicated that the tissue was as “densely packed” as normal brain tissue and retained a high degree of directional organisation. However, the deviation of the principal eigenvector in this region indicated that diffusion, although still directional, had taken a different orientation. Experimental studies have shown that water diffuses primarily parallel to fibres.10 Our findings therefore suggest a displacement (rather than a destruction) of fibres and showed that the tumour had a distant mass effect on normal appearing white matter. This information could not be obtained by other means. Our finding explains why the patient had only a mild neurological deficit despite the large tumour located in the expected position of the pyramidal tract.

With DTI the microstructural organisation in the brain can be investigated which may facilitate the detection of structural abnormalities in normal appearing white matter—for example, in multiple sclerosis.11 Displaying the principal eigenvector to study the direction of fibres allows the identification of abnormalities in the normal appearing white matter. This will improve the understanding of the organisation of fibres in the white matter of patients, not only in tumours but also in other abnormalities such as dysgenesis. The technique has the prospect of guiding neurosurgical interventions.12 Several different diffusion parameters and display techniques have been used to describe and visualise the directionality of white matter.13-16 We demonstrated a deviation of fibres in normal appearing white matter in a patient compared with spatially normalised control brains by visual (qualitative) means. In the future quantitative methods to assess displacement can be developed. The technique will assist the interpretation of neurological findings and functional imaging studies.

Acknowledgments

This research has been kindly funded by a European Community Fellowship (UCW), by the National Society for Epilepsy (MRS), the Brain Research Trust (CAC and MRS), and the Multiple Sclerosis Society of Great Britain and Northern Ireland (GJB and GJMP). We thank Dr B E Kendall, Dr J M Stevens, and Dr J S Duncan for their helpful comments.

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