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Cervical dystonia is associated with a polymorphism in the dopamine (D5) receptor gene

Abstract

The objective was to assess whether polymorphisms in the dopamine receptor and transporter genes are associated with development of primary cervical dystonia.

A case-control allelic association study is described of 100 patients with cervical dystonia and 100 controls using polymorphisms within D1-5 receptor and dopamine transporter genes.

No significant association was found between patient and control allele frequencies for polymorphisms in genes for the D1 to 4 receptors and dopamine transporter. Significant associations, however, were found for alleles 2 and 6 of the D5 receptor micosatellite. Carriage of allele 2 was associated with cervical dystonia, whereas allele 6 was overrepresented in the control group, implying a possible protective effect. The association with allele 6 remained significant after Bonferroni correction.

In conclusion, the finding of a significant association with an allele in the D5 receptor gene in patients with cervical dystonia may indicate a pathogenic role of this gene (or neighbouring genes). Further studies are required to confirm this finding and to assess whether these alleles are part of distinct haplotypes associated with other polymorphisms imparting a functional effect on the D5 receptor.

  • cervical dystonia
  • D5 dopamine receptor
  • allelic association

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Cervical dystonia is the commonest form of primary focal dystonia and is characterised by involuntary muscle contractions of the cervical musculature.1The cause of focal dystonia is unknown, although there is evidence for a genetic component to its aetiology,2particularly for cervical dystonia.3 A gene was mapped to chromosome 18p (DYT7) in a large German family in whom the phenotype was predominantly adult onset torticollis.4A follow up study of 15 apparently sporadic cases of focal dystonia in the same region of northwest Germany suggested linkage disequilibrium for a haplotype of genetic markers from this chromosomal region, implying a founder mutation from a common ancestor.5A second study in a larger population, however, was unable to replicate this finding.6

Evidence has accumulated to suggest that abnormalities of dopaminergic neurotransmission in the basal ganglia may be responsible for producing dystonic movements. Dopa responsive dystonia (a rare dominantly inherited form) is caused by mutations in the gene for GTP-cyclohydrolase I, the rate limiting enzyme for the synthesis of tetrahydrobiopterin, a key cofactor in dopamine synthesis.7The DYT1 gene which causes childhood onset autosomal dominant primary generalised dystonia has recently been cloned and encodes a protein called torsin A.8The function of torsin A is unknown, but it is preferentially expressed in the substantia nigra pars compacta9and may interfere with dopamine vesicle transport.10Study of a single postmortem case of DYT-1 dystonia found normal nigral cellularity and striatal dopamine concentrations that were within the control range, except for a reduction in rostral portions of the putamen and caudate nucleus.11This supports a functional dopaminergic deficit rather than alterations in absolute dopamine concentrations.

Further evidence for dopaminergic abnormalities in dystonia comes from the study of primates treated with the nigral toxin MPTP12and also of patients with tardive dystonia caused by neuroleptic dopamine receptor antagonists.13In addition, a SPECT study in patients with cervical dystonia using [123I] epidepride, a D2 receptor marker, showed evidence of reduced striatal binding.14

The aim of this study, therefore, was to examine if polymorphisms in the genes for the five dopamine receptor or dopamine transporter (DAT1) genes are associated with cervical dystonia.

Patients and methods

One hundred patients with cervical dystonia were identified from movement disorder or botulinum toxin clinics of the participating centres. Each patient had been seen by a neurologist with expertise in the field of dystonia and had been diagnosed as having primary cervical dystonia for at least 1 year. Primary dystonia was distinguished from secondary dystonia using the criteria of Marsden and Harrison.15This relies on the absence of neurological features other than dystonia and the absence of known precipitants. In most cases no other investigations were performed, except copper studies. Patients with secondary dystonia or those with affected first degree relatives were excluded. All cases were British and to avoid bias from studying a mixture of ethnic groups, were white. One hundred control subjects with a similar age range, sex, and ethnic background were collected from patients attending the Royal Free Hospital who did not have a neurological illness. The study was undertaken after ethics committee approval from the Royal Free Hospital.

Genomic DNA was extracted from lymphocytes (Nucleon BACC2 kit, Amersham) and the polymorphisms amplified by polymerase chain reaction (PCR) as previously described. The PCR was carried out in a total volume of 25 μl using 100 ng genomic DNA, 20 pmol of each primer, 200 μM of each dNTP, 1.5 mM MgCl2, and 1unit of Red Hot Taq with its standard buffer (Advanced Biotechnologies). D2 repeat, D2 promoter, and D4 and D5 repeat polymorphisms required an additional 10% DMSO. The 10 polymorphisms selected and method of detection are shown in table 1.

Table 1

Dopamine receptor and transporter (DAT1) polymorphisms

Statistical analysis was performed using a χ2 test. Bonferroni correction was applied where appropriate.

Results

The mean (SD) age of patients with cervical dystonia and controls was 55.5 (12.1) and 52.8 (17.5) years respectively. Mean age at onset of cervical dystonia was 44.6 (14.2) years. Sex ratios (female:male) were 1.7 for the patient group and 1.6 for the control group. There were no significant differences in the age and sex distributions between patients with cervical dystonia and the control group.

Table 2 shows the distribution of single nucleotide polymorphisms in D1-3 receptors for patients with cervical dystonia and controls. Table 3 compares the allele frequencies for microsatellites in D2, D4, D5, and DAT1 genes in cervical dystonia and control populations. No significant association was found between patient and control allele frequencies for polymorphisms in the D1 to 4 receptors and dopamine transporter. Significant associations, however, were found for alleles 2 (p=0.004) and 6 (p=0.0003) of the D5 receptor microsatellite. If corrected for multiple comparisons using the Bonferroni method only the result for D5 allele 6 remained significant. Hardy-Weinberg equilibrium was demonstrated in the control group for all polymorphisms studied.

Table 2

Results for allelic association for cervical dystonia and SNPs in D1-3 receptor genes

Table 3

Allelic association between cervical dystonia and microsatellites in dopamine receptor and DAT genes

Discussion

These data indicate an association between cervical dystonia and a polymorphism in the D5 receptor gene. For the most significant result, there is overrepresentation of allele 6 of the D5 receptor dinucleotide repeat within the control group compared with the cervical dystonia patient group. This may imply a protective effect of carriage of this particular allele. The converse is true for allele 2 where there is an overrepresentation in the cervical dystonia group. It is unlikely that the dinucleotide repeat within the D5 receptor confers a functional effect on the protein and any possible association may represent linkage disequilibrium with a different polymorphism within the same gene or even another gene in close proximity. Thus it is possible that there is a haplotype associated with allele 2 that confers susceptibility to developing cervical dystonia and a “protective” haplotype associated with allele 6.

D5 belongs to the D1-like class of dopamine receptors (D1/D1A, D1B) that stimulate adenlyl cyclase activity. For this reason it is interesting to note the trend towards significance for association with the D1.1 allele 1 (p= 0.056). D1 receptors are expressed in the striatum as well as cortex and limbic structures. D5 receptor mRNA has been demonstrated in human substantia nigra pars compacta as well as the neocortex and hippocampus.23Thus D5 receptor dysfunction could influence basal ganglia dopaminergic pathways. If there is an association between D1/D5 receptors for cervical dystonia it is possible that this effects the direct pathway in the cortico-basal ganglia-cortex motor loops leading to overactivity and a susceptibility for developing cervical dystonia.

Evidence to support a possible dopaminergic susceptibility for developing focal dystonia comes from recent work on a rat model of blepharospasm, a focal dystonia of the extraocular muscles.24Rats were given a striatal injection of OH-dopa, leading to a dopaminergic lesion which produced excitability of the trigeminal nerve blink reflex. This in itself did not produce blepharospasm, but a subsequent lesion to the facial nerve, weakening the orbicularis oculi, led to the development of blepharospasm. Thus it is possible that a functional dopaminergic genetic susceptibility may subsequently lead to the development of cervical dystonia after appropriate secondary stimuli.

For all allelic association studies it is critical that any possible allelic association is confirmed in an independent cohort and control group, and we are currently undertaking this. In addition, we are searching for other single nucleotide polymorphisms or polymorphisms within the D1 and D5 genes to see whether it is possible to create a haplotype associated with alleles 2 and 6 of the D5 repeat and the D1.1 single nucleotide polymorphism. This may provide evidence for linkage disequilibrium to a different functional polymorphism or mutation in these genes, or even to a gene located in close proximity.

Acknowledgments

We are grateful to The Dystonia Society for financial assistance, and all the patients who participated in this study.

References

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