The effect of the potassium channel blocker 4-aminopyridine (4-AP) on spontaneous upbeat nystagmus (UBN) was investigated with the search coil technique during fixation in different gaze positions and smooth pursuit in a patient before and after ingestion of 10 mg 4-AP. UBN was reduced from 8.6 deg/s to 2.0 deg/s by 4-AP causing subjective relief from distressing oscillopsia, and impaired upward smooth pursuit was restored (gains: before medication 0.38; after medication 0.86). In the dark, UBN was slightly stronger and not affected by 4-AP. We propose that 4-AP improved the function of cerebellar pathways that mediate gaze holding and smooth pursuit by intensifying the excitability of cerebellar Purkinje cells.
- 4-AP, 4-aminopyridine
- DBN, downbeat nystagmus
- UBN, upbeat nystagmus
- upbeat nystagmus
- potassium channel blocker
- smooth pursuit
- case report
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Spontaneous upbeat nystagmus (UBN)—that is, upbeat nystagmus with gaze straight ahead, is an ocular motor disorder which manifests with oscillopsia due to retinal slip of the visual scene and postural instability. UBN usually increases with upgaze1 and is associated with impaired upward pursuit.2 It can be caused by lesions at several separate and distinct sites such as the pontomesencephalic junction,1 medulla,2–5 perihypoglossal nuclei,6 anterior cerebellar vermis,7 ventral tegmentum,8 and brachium conjunctivum.9 Lesions in the pathways mediating upward eye movements—for example, from the vestibular nuclei through the brachium conjunctivum to the ocular motor nuclei, might result in slow downward drift of the eyes that is repeatedly corrected by fast movements in the reverse direction. Although its causative mechanism is still not known, UBN is hypothesised to reflect an imbalance of vertical vestibulo-ocular reflex tone,9,10 as suggested for downbeat nystagmus (DBN). Alternatively, a mismatch in the neural coordinate systems of saccade generation and neural velocity-to-position integration due to deficiency of the latter might cause UBN, as has been suggested for DBN.11
GABAergic substances such as baclofen12 have been used to treat UBN and DBN, but with only moderate success. Recently 3,4-diaminopyridine13 and 4-aminopyridine (4-AP)14 were shown to suppress DBN effectively. These potassium channel blockers are known to cancel the A current and the delayed rectifier, as well as other potassium channels.15 Animal experiments have recently shown that 4-AP increases the excitability of cerebellar Purkinje cells.16 Both drugs are thought to influence DBN by increasing the physiological inhibitory influence of the vestibulocerebellum on the vestibular nuclei.13,15 This prompted us to test the effects of 4-AP on UBN by measuring smooth pursuit and gaze holding with the search coil technique in a patient with UBN.
A 22 year old man noted sudden onset of blurred vision, which he described as “running downward of visual images”, 24 hours after hitting his head on a beam when suddenly standing up from a sitting position. Neurological examination six weeks later was normal except for UBN in gaze straight ahead (visual acuity: right eye 0.33, left eye 0.25). UBN increased during convergence, and upward and lateral gaze to the left, and decreased in downward gaze. Upward smooth pursuit was impaired in the form of cogwheel movements. Psychophysical testing revealed a slight tilt of the subjective visual vertical to the right. Saccades were normal. Magnetic resonance imaging (MRI) (including high resolution MRI of the brainstem and cerebellum and fast echo planar diffusion weighted images), blood chemistry, and analysis of cerebrospinal fluid were also normal. Brainstem auditory evoked potentials on the left side were delayed for interpeak latency of waves III and V, suggesting a mesencephalic lesion.17 Thus, UBN in this patient might have been caused by a small lesion of the midbrain.
4-AP was first administered six weeks after the accident, the patient took 10 mg 4-AP daily for eight weeks. The medication was discontinued in view of gradual recovery (visual acuity: both eyes 1.0).
Eye movement recordings
The patient gave his written informed consent for the recordings which had been approved by the local ethics committee of the Medical Faculty of the University of Munich.
Three dimensional eye movements were recorded six weeks after the accident, before and 90 minutes after ingestion of 10 mg 4-AP, and at a follow up examination another nine weeks later but without medication. Dual search coils (Skalar, Delft, the Netherlands) on the left eye (after topical anaesthesia with oxybuprocain-HCl) and on the forehead were used to determine eye and head positions from the voltage induced by three orthogonal magnetic fields (Remmel, Ashland, MA, USA). The signals were digitised with a 12 bit analogue–digital converter at a sampling rate of 1 kHz. The three dimensional position of the eye and the slow phase velocity of the nystagmus were evaluated in nine horizontal–vertical eye positions, with the patient sitting in an upright position as described elsewhere.11 Eye movements were recorded in the dark while showing a continuously visible or a flashed target (visible for 750 ms before and after the target jump, dark period 2.5 s) to avoid visual interference by fixation of the target. Horizontal and vertical smooth pursuit was tested by having the patient follow a moving laser dot (size 0.1°; frequency 0.1667 Hz, amplitude ±18°) at the centre of a screen placed 140 cm in front of the subject. Gain values were determined by linear regression of slow phase velocity to target velocity for positive and negative target velocities separately.
The patient reported less oscillopsia 45 minutes after ingestion of 4-AP. The effect lasted for more than three hours without any side effects.
Effects of 4-AP on UBN and eye position dependence with visible target
4-AP reduced slow phase velocity of UBN with gaze straight ahead from a mean (SD) 8.6 (0.2) deg/s before treatment to 2.0 (0.1) deg/s 90 minutes after treatment when the target remained visible (fig 1A, upper panel). Multiple regression analysis11 of slow phase velocity in different eye positions revealed that before treatment, the slow phase velocity depended on eye position in accordance with Alexander’s law (fig 1B upper left panel; drift time constants: vertical 8.5 s, horizontal 22.4 s). This implies gaze evoked vertical nystagmus. Ninety minutes after medication, the residual drifts violated Alexander’s law (fig 1B, upper right panel; time constant −13.0 s)—that is, UBN increased with downward gaze.
Nine weeks later, the patient no longer had oscillopsia. The follow up experiment without medication showed a mean slow phase velocity of UBN of 3.0 (0.1) deg/s (time constant −18.2 s).
Effects of 4-AP on UBN and eye position dependence in the dark with flashed target
4-AP had almost no effect on UBN during attempted fixation in darkness (fig 1A, middle panel; before medication: 12.0 (0.1) deg/s, after medication: 10.0(0.1) deg/s). Vertical eye position dependence of slow phase velocity violated Alexander’s law before and after medication (fig 1B lower panel; before medication: −9.3 s, after medication: −6.3 s). Nine weeks later without medication, UBN in darkness was still larger than during fixation (6.2 (0.1) deg/s, time constant −22.1 s).
Effects of 4-AP on smooth pursuit in UBN
Vertical smooth pursuit was highly asymmetrical before medication (fig 1A, bottom panel; upward gain 0.38, downward gain 0.74). Upward smooth pursuit improved 90 minutes after medication (upward 0.89, downward 0.86). Horizontal smooth pursuit was normal before medication (rightward 0.98, leftward 0.95) and slightly increased afterwards. Nine weeks later, vertical smooth pursuit without medication was only slightly impaired (upward 0.82, downward 0.91).
The potassium channel blocker 4-AP substantially suppressed UBN in our patient during fixation in the light, but not in the dark, and improved upward vertical smooth pursuit. Accordingly, the patient felt relieved of distressing oscillopsia. The question arises as to how the medical effects are related to the pathomechanism of UBN. A cerebellar involvement is likely, since the ability of 4-AP to block several potassium currents—for example, the A current, the delayed rectifier, and calcium dependent potassium currents,18 leads to an increase of the excitability of cerebellar Purkinje cells.16
Eye position dependent UBN was smaller with a continuously visible fixation target than in the dark, even without medication. This suggests that visual input still had an impact on gaze holding (fig 1B, left column). 4-AP increased this influence so that UBN was nearly suppressed by visual input, but not in the dark (fig 1B, right column). We therefore assume that the concurrent improvement in smooth pursuit performance relied on the same visual mechanism mediated by 4-AP. Hence, 4-AP was unable to suppress UBN in darkness, but obviously activated pathways carrying visual information, which then could be used for UBN suppression in light.
Visual information for pursuit and nystagmus suppression reaches the Purkinje cells of the cerebellar flocculus and paraflocculus via the dorsolateral pontine nuclei and the mossy fibres, and additionally via the lateral terminal nuclei of the accessory optic system and the climbing fibres of the inferior olive.19 Since nystagmus suppression and upward pursuit were initially defective, it must be assumed that the lesion not only caused UBN in darkness, but also affected pathways carrying visual information for oculomotor control. It is unlikely that 4-AP restored these pathways. Rather, we propose that 4-AP helps to activate parallel pathways that can take over the function of the lesioned structures. This parallel pathway may be strengthened by 4-AP by increasing the excitability of cerebellar Purkinje cells.16 It may also evoke complex spikes in these cells similar to those elicited by climbing fibre stimulation.20
In summary, 4-AP may be an efficient agent for the treatment of UBN. Despite the striking effect of 4-AP in our patient, it remains to be tested in other patients with UBN due to various lesions to determine whether this compound is useful for all types of UBN with distressing oscillopsia.
We thank T Eggert for maintenance of the coil system, J Benson for copyediting the manuscript, and M Glaser for orthoptic examination of the patient.
↵* These authors contributed equally to the work.
This work was supported by the Deutsche Forschungsgemeinschaft (GL 342/1-1) and the Fritz-Thyssen-Stiftung.
Competing interests: none declared