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Serum glial fibrillary acidic protein as a biomarker for intracerebral haemorrhage in patients with acute stroke
  1. C Foerch1,
  2. I Curdt2,
  3. B Yan3,
  4. F Dvorak1,
  5. M Hermans1,
  6. J Berkefeld3,
  7. A Raabe4,
  8. T Neumann-Haefelin1,
  9. H Steinmetz1,
  10. M Sitzer1
  1. 1Department of Neurology, Johann Wolfgang Goethe-University, Frankfurt am Main, Germany
  2. 2Roche Diagnostics GmbH, Centralised Diagnostics, Penzberg, Germany
  3. 3Institute of Neuroradiology, Johann Wolfgang Goethe University
  4. 4Department of Neurosurgery, Johann Wolfgang Goethe University
  1. Correspondence to:
 Dr Christian Foerch
 Department of Neurology, Johann Wolfgang Goethe University Frankfurt am Main, Schleusenweg 2-16, D-60528 Frankfurt am Main, Germany; foerch{at}em.uni-frankfurt.de

Abstract

Background: Biomarkers of stroke are an evolving field of clinical research. A serum marker which can differentiate between haemorrhagic and ischaemic stroke in the very early phase would help to optimise acute stroke management.

Objective: To examine whether serum glial fibrillary acidic protein (GFAP) identifies intracerebral haemorrhage (ICH) in acute stroke patients.

Methods: A pilot study assessing 135 stroke patients admitted within six hours after symptom onset. Diagnosis of ICH (n = 42) or ischaemic stroke (n = 93) was based on brain imaging. GFAP was determined from venous blood samples obtained immediately after admission, using a research immunoassay.

Results: GFAP was detectable in the serum of 39 patients (34 of 42 (81%) with ICH, and five of 93 (5%) with ischaemic stroke). Serum GFAP was substantially raised in patients with ICH (median 11 ng/l, range 0 to 3096 ng/l) compared with patients with ischaemic stroke (median 0 ng/l, range 0 to 14 ng/l, p<0.001). Using receiver operating characteristic curve analysis, a cut off point of 2.9 ng/l provided a sensitivity of 0.79 and a specificity of 0.98 for the identification of ICH in acute stroke (positive predictive value 0.94, negative predictive value 0.91; p<0.001).

Conclusions: Serum GFAP can reliably detect ICH in the acute phase of stroke. Further evaluation of the usefulness of GFAP as an early diagnostic marker of ICH is now required, with the aim of optimising cause specific emergency management.

  • GFAP, glial fibrillary acidic protein
  • ICH, intracerebral haemorrhage
  • NIHSS, National Institutes of Health stroke scale
  • biological marker
  • intracerebral haemorrhage
  • cerebral ischaemia
  • acute management

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Footnotes

  • Published Online First 20 September 2005

  • Competing interests: Christian Foerch and Matthias Sitzer are designated as inventors in the European patent application cited below. Ingo Curdt was employed as a scientific trainee by Roche Centralised Diagnostics, 82377 Penzberg, Germany; he is also designated as an inventor in the European patent application cited below. Title of pending patent: “Use of GFAP for identification of intracerebral haemorrhage” (patent application number: 03021571.9; date of filing: 24 September 2003. Bernard Yan, Florian Dvorak, Marcella Hermans, Joachim Berkefeld, Andreas Raabe, Tobias Neumann Haefelin, and Helmuth Steinmetz declare they have no competing interests.

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