J Neurol Neurosurg Psychiatry 79:1376-1381 doi:10.1136/jnnp.2008.151126
  • Research paper

The use of nerve and muscle biopsy in the diagnosis of vasculitis: a 5 year retrospective study

Open Access
  1. D L H Bennett1,2,
  2. M Groves2,
  3. J Blake2,4,
  4. J L Holton2,
  5. R H M King3,
  6. R W Orrell3,
  7. L Ginsberg3,
  8. M M Reilly2
  1. 1
    Wellcome Clinical Scientist Fellow King’s College and University College London, London, UK
  2. 2
    MRC Centre for Neuromuscular Diseases, Department of Molecular Neurosciences, National Hospital for Neurology and Neurosurgery and Institute of Neurology, London, UK
  3. 3
    Department of Clinical Neurosciences, Institute of Neurology, Hampstead Campus, University College London and Royal Free Hospital, London, UK
  4. 4
    Department of Neurophysiology, Norfolk and Norwich University Hospital, Norwich, UK
  1. D L H Bennett, Department of Neurorestoration, CARD Wolfson Wing, Hodgkin Building, Guy’s Campus, King’s College London, London Bridge, London SE1 1UL, UK; david.bennett{at}
  • Received 4 April 2008
  • Revised 3 August 2008
  • Accepted 14 August 2008
  • Published Online First 26 September 2008


Introduction: Peripheral nerve vasculitis is an important condition which can be diagnostically challenging and is one of the principal current indications for nerve and muscle biopsy. Previous studies have suggested that combined nerve and muscle biopsy (usually of the superficial peroneal nerve and peroneus brevis muscle) produces a higher diagnostic yield than nerve biopsy alone in the investigation of vasculitis.

Objective: To determine whether in our two centres combined nerve (usually the sural) and muscle (usually the vastus lateralis) biopsy improved diagnostic yield compared with nerve biopsy alone.

Methods: We interrogated our database of all nerve biopsies (usually of the sural nerve) performed at our institutions over 5 years and identified 53 cases of biopsy proven peripheral nerve vasculitis. Clinicopathological and neurophysiological data in these patients were reviewed.

Results: The most common clinical presentation was with a painful asymmetric axonal polyneuropathy or mononeuritis multiplex (66% of cases). Nerve biopsy demonstrated definite vasculitis in 36%, probable vasculitis in 62% and no vasculitis in 2% of cases. In 24 patients a muscle biopsy (usually the vastus lateralis) was also performed and vasculitis was demonstrated in 46% of these (in 13% showing definite and 33% probable vasculitis). There was only one patient in whom vasculitis was demonstrated in muscle but not in peripheral nerve.

Conclusion: Combined nerve (usually sural) and vastus lateralis muscle biopsy did not significantly increase the diagnostic yield compared with nerve biopsy alone. A sensible approach to the diagnosis of peripheral nerve vasculitis is to choose a nerve to biopsy which is clinically affected and amenable to biopsy. If the sural nerve is chosen, the data suggest that it is not routinely worth doing a vastus lateralis biopsy at the same time, whereas if the superficial peroneal nerve is chosen, it seems appropriate to do a combined superficial peroneal nerve and peroneus brevis biopsy. It is still not known if both the sural and superficial peroneal nerves are involved clinically which one gives the higher yield if biopsied.


  • See Editorial Commentary, p 1307

  • Supplementary tables 1–3 are published online only at

  • Funding: MR is grateful to the Medical Research Council (MRC) and the Muscular dystrophy campaign (MDC) for their support. DB is a Wellcome Trust intermediate clinical scientist fellow. Part of this work was undertaken at University College London Hospitals/University College London, which received a proportion of funding from the Department of Health’s National Institute for Health Research Biomedical Research Centres funding scheme.

  • Competing interests: None.

  • Ethics approval: This study was performed as an audit and presented at the hospital (National Hospital for Neurology and Neurosurgery) audit meeting.

Open Access

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