Article Text


Retinal microvascular abnormalities and stroke: a systematic review
  1. F N Doubal1,
  2. P E Hokke2,
  3. J M Wardlaw1
  1. 1
    Division of Clinical Neurosciences, University of Edinburgh, Western General Hospital, Edinburgh, UK
  2. 2
    Academic Medical Centre, University of Amsterdam, Amsterdam, The Netherlands
  1. Dr F N Doubal, Division of Clinical Neurosciences, University of Edinburgh, Bramwell Dott Building, Western General Hospital, Edinburgh EH4 2XU, UK; fergus.doubal{at}


Background: Lacunar strokes account for 25% of ischaemic strokes, but their precise aetiology is unknown. Similarities between the retinal and cerebral small vessels mean that clarification of the exact relationship between retinal microvascular abnormalities and stroke, and particularly with stroke subtypes, may aid understanding of the aetiology of lacunar stroke and stroke risk.

Methods: A systematic review of the literature was performed by searching Medline and Embase to October 2007 for studies in humans that investigated the association between retinal microvascular abnormalities and prevalent or incident stroke. Data and calculated summary risk ratios (sRR) were extracted for associations between retinal microvascular abnormalities and stroke, including stroke subtypes where possible, adjusted for key variables. 37 papers from 22 different studies were included with 62 975 subjects (mean age 62 years) among whom there were 2893 strokes. Stroke identification and diagnosis methods varied.

Results: Retinopathy was associated with incident stroke (sRR 2.1, 95% CI 1.7 to 2.6) and prevalent stroke (sRR 2.5, 95% CI 1.4 to 4.3). Incident stroke was also associated with retinal artery embolism (sRR 2.9, 95% CI 1.6 to 5.1) and venular widening (sRR 1.4, 95% CI 1.1 to 1.7). There was significant heterogeneity between studies for some associations. There were no data on retinal microvascular abnormalities and haemorrhagic versus ischaemic stroke or ischaemic stroke subtypes.

Conclusions: Retinal microvascular abnormalities are associated with stroke, but more data are required to clarify associations between specific types of retinal microvascular abnormality and stroke, as well as between different stroke subtypes. Future retinal-stroke studies should concentrate on carefully diagnosing and accurately sub-typing ischaemic stroke.

Statistics from

Lacunar ischaemic stroke accounts for 25% of all ischaemic strokes,1 but its precise aetiology is unknown.2 Possible causes include atherothromboembolism,3 intracranial large artery stenosis, intrinsic microvascular atheroma, or endothelial dysfunction manifesting either through ischaemia or failure of the blood–brain barrier.4

The cerebral blood vessels that, when diseased, give rise to lacunar stroke share many characteristics with retinal vessels,5 being developmentally related during embryogenesis and of similar diameter (50–250 µm). Retinal vessels are visibly affected by known risk factors for stroke such as diabetes and hypertension.6 Therefore, determining any association between retinal microvascular abnormalities and stroke may improve understanding of the nature of the cerebral small vessel changes underlying lacunar ischaemic stroke and aid cerebrovascular and cardiovascular risk profiling.

Several studies have examined a possible link between stroke and the retina, including large epidemiological studies in community-dwelling populations and small detailed studies of patients with specific diseases attending tertiary hospitals. The presence and strength of any reported associations between retinal vascular abnormalities and stroke vary considerably, possibly reflecting the differing populations, methodologies, definitions of disease and abnormalities sought.

Therefore, we performed a systematic review to clarify associations between a range of retinal microvascular abnormalities and any stroke and with specific subtypes of both stroke and ischaemic stroke. We also determined where more information was needed from new studies.


Search strategy

We searched Ovid Medline (from 1 January 1950) and Embase (from 1 January 1980) to 5 October 2007 for papers investigating associations between retinal microvascular abnormalities and stroke (defined as stroke/transient ischaemic attack/asymptomatic brain infarct identified on imaging). The electronic search was supplemented by the authors’ personal files, hand searching the bibliographies of papers selected from the electronic search, checking references of review articles and by surveillance of key journals from April 2005 to October 2007 (Neurology, Lancet, Brain, Ophthalmology, Stroke, British Journal of Ophthalmology, BMJ, Retina). Two reviewers (FD and PH) independently conducted the searches, paper selection and data extraction. A third reviewer (JW) assessed a sample of papers for inclusion, extracted data and adjudicated in any disagreements.

Inclusion criteria

We included studies published in full which assessed the association between retinal microvascular abnormalities and stroke in adult humans.

Exclusion criteria

We excluded studies published only as abstracts or presented in conferences without full subsequent publication, review papers, papers dealing with specific single gene-related disorders such as CADASIL, papers studying only macular degeneration and duplicate publications. We excluded papers dealing with retinal microvascular abnormalities and only cerebral white matter disease (leucoaraosis) seen on imaging.


Stroke is defined as a focal sudden neurological deficit of presumed vascular aetiology with brain imaging excluding other causes.7 We defined “stroke” as either a clinically diagnosed stroke or transient ischaemic attack (with or without cerebral imaging) or a cerebral infarct identified on brain imaging without definite associated clinical features being documented. We defined “incident stroke” as strokes which occurred after the patient/subject had been enrolled in the study and “prevalent stroke” as strokes which had preceded patient enrolment in the study.

“Retinal microvascular abnormalities” included retinopathy (microaneurysms, soft and hard exudates, haemorrhages), retinal arteriolar and venular width measurements (arterio-venous ratios (AVR), focal arteriolar narrowing (FAN), arterio-venous nicking (AVN)), retinal artery (RAO) or vein occlusion (RVO), retinal artery emboli and alterations in retinal vascular geometry (angles or vessel tortuosity).

Paper assessment

We collected data on a form designed and piloted specifically for this review. We recorded study population details (age, selection criteria, co-morbidities), study design and assessed definitions of stroke and retinal outcomes.

We assigned the reference standard for retinal assessment as high-quality retinal photographs of both eyes (preferably multifield) and blinded assessment of the retinal appearance with a standardised and externally validated tool. We collected data on which retinal features were measured, methods of retinal assessment, use of mydriatics, number of fields imaged per eye and which eyes were photographed, type of camera and field of view.

The reference standard for stroke diagnosis was assessment of the patient by a stroke specialist at the time of the stroke with appropriate cerebral imaging to differentiate haemorrhagic from ischaemic stroke and stroke mimic and, if ischaemic, to identify whether lacunar or large artery ischaemic stroke. We considered the following methods of stroke diagnosis to be less reliable: patient questionnaires, retrospective case note analysis, death certificates and review of centrally held health statistics. We assessed studies for the method of subject selection, the presence of a suitable control group, subtyping of stroke and blinding of assessment of images/patients. We extracted data on numbers with stroke or transient ischaemic attack (TIA) and different retinal features in as much detail as possible, any calculated odds ratios, relative risks or hazards ratios and whether these were unadjusted or adjusted for shared risk factors such as hypertension or diabetes. When counting total numbers of patients and stroke events that contributed to each analysis, we were careful to count patients from studies contributing more than one paper only once to avoid unnecessary bias. We did not have the resources to contact authors of studies to ask regarding missing data or individual patient data.

Statistical analysis

We summarised the numbers of subjects in the different population samples, with or without various retinal abnormalities and stroke. Stroke outcomes were rare (incidence less than 10%), and odds ratios, hazards ratios and relative risk are considered to be equivalent. We converted all ratios to summary risk ratios (sRR) and prepared Forrest Plots using Review Manager Software Version 5 (Cochrane, Oxford) and Excel (version 2003; Microsoft, Redmond, Washington). We used a random effects generic inverse variance method to plot sRR and 95% CIs using a logarithmic scale from the adjusted ratios and 95% CI. We used adjusted ratios in preference to unadjusted to best account for differences in risk factors between populations. We tested for associations between stroke and predefined retinal microvascular abnormalities, and then separately examined associations in important predefined subgroups such as incident or prevalent stroke, ischaemic versus haemorrhagic stroke and ischaemic stroke subtypes. We tested for heterogeneity between study results with the χ2 test for heterogeneity with an alpha level for significance set at p = 0.05. When heterogeneity was detected, we investigated the methodology and definitions of variables used in the heterogeneous studies to identify possible causes.


Included studies

The initial search produced 4461 titles and abstracts, subsequent screening of which yielded 52 potentially relevant papers describing probable retinal microvascular abnormalities and stroke for which we assessed the full texts. Of the 52, 15 were excluded (13 were not relevant, and two were in Japanese and not easily translated), leaving 37 papers which met the inclusion criteria. Table 1 documents the main findings of the included papers.

Table 1 Descriptions of included studies arranged by stroke outcome

Description of included studies

Of the 37 papers, 21 originated from large population-based cohort studies (table 2, fig 1).828 Of the remaining 16 papers, 14 originated from hospital-based studies,2942 one from within a trial of blood pressure reduction43 and one from a World Health Organization (WHO) study of follow-up in diabetics.44 The papers were from the US, UK, Australia, Japan, Korea, The Netherlands, Saudi Arabia, Greece, Switzerland, Poland, Germany, Croatia, Hong Kong and Cuba.

Figure 1 Summary risk ratios (sRR) showing association of incident stroke and different retinal microvascular abnormalities. The size of the square denotes the weight attributed to each paper, and the horizontal lines represent the 95% CI. A diamond represents sRR with the width representing the 95% CI. An arrowhead mean that the upper CI is not marked on chart. The right-hand column indicates key adjustments. BP, blood pressure; DM, diabetes mellitus. *Studies which used only radiological diagnosis of infarct; ¥BMES/BDES combined.
Table 2 Background information of population-based studies contributing to the review

The 37 papers include 62 975 subjects. The mean age of the patients in the 31/37 papers which provided this information was 62.5 years (SD 7.7). Among the 62 975 subjects, 1900 had a clinically evident stroke (total of incident and prevalent stroke), and 993 had infarction seen on brain imaging with no stated clinical correlate. We were unable to extract the number of strokes from 6/37 papers.19 23 25 36 38 39 The number of strokes per paper varied from eight to 496.10 32

Ascertainment of stroke

Twenty-one papers examined incident stroke8 1115 1822 24 2628 3944 (total population 34 894; 1442 (4.1%) strokes during follow-up). Fifteen papers examined prevalent stroke9 16 17 23 25 2938 (total population 21 950; 501 (2.2%) had prior stroke), and one paper examined both prevalent and incident stroke.10

The definition of stroke varied between the papers. Thirty of the 37 papers defined “stroke” in the index paper or related publications.816 1824 2630 3240 Nine of the 37 papers used asymptomatic infarcts seen on brain imaging.9 10 27 2931 3638 The other 28 papers assessed some measure of clinical stroke of which only 9/28 papers subtyped stroke into haemorrhagic or ischaemic.8 14 15 23 26 3234 40 Of these nine papers, six further subtyped ischaemic strokes,8 14 15 23 34 40 but only one paper reported retinal microvascular abnormality differences between the ischaemic stroke subgroups.34

The methods of stroke identification varied widely. Two papers used specialist examination at the time of stroke;34 40 12 papers used clinical assessment but not necessarily at the time of the stroke by study investigators;11 16 17 20 23 25 32 33 35 39 43 44 14 papers used phone interviews, review of case notes or death certificates;8 1215 18 19 21 22 24 26 28 41 42 and nine papers used brain imaging without clinical correlation.9 10 27 2931 3638

Ascertainment of retinal microvascular abnormalities

The retinal assessment techniques were well described and performed. Twenty-seven of 37 performed retinal photography;829 34 37 38 40 43 six performed direct ophthalmoscopy;31 32 35 41 42 44 two used hospital records;33 39 and only two did not record the method of retinal assessment.30 36 Twenty-two of 37 papers used retinal abnormality classifications that had been validated either internally or externally.829 Of the 27 papers which used retinal photography, the number of fields used and the angle of the field of view varied: four papers gave no information regarding which retinal fields were taken,29 34 37 40 15 took one field centred on the optic disc and macula,9 10 1316 18 19 21 23 24 2628 38 and eight took more than one field.8 11 12 17 20 22 25 43 Eight of 27 papers gave no information regarding the angle of the field of view used,17 20 22 29 34 37 40 43 two papers used 20°,26 27 eight papers 30°,1113 19 21 24 25 28 eight papers 45°810 1416 18 23 and one paper 50°.38

The retinal features that were assessed varied greatly, the most common being retinopathy (19/37 papers) defined as any of the following: microaneurysms, haemorrhages or exudates.811 1417 19 20 22 2933 39 43 44 Two papers divided retinopathy into its constituent parts and presented separately.9 10 Other papers used retinal artery occlusion/emboli (6/37 papers);12 21 24 36 40 42 retinal vein occlusion (5/37 papers);25 28 35 36 41 vessel calibre of either the venules or arterioles, or generalised or focal narrowing (9/37 papers);911 13 14 18 23 26 27 arteriovenular nicking(5/37 papers);911 14 23 retinal geometry (2/37 papers);13 38 or retinal artery sclerosis (3/37 papers).34 37 38

Association between retinal microvascular abnormalities and stroke

We were able to extract data and combine risk ratios for 24 papers (total study population 39 376, 1696 stroke (figs 1, 2).

Figure 2 Summary risk ratios (sRR) showing association of prevalent stroke and different retinal microvascular abnormalities. The size of the square denotes the weight attributed to each paper, and the horizontal lines represent the 95% CI. Diamonds represent the sRR, and the width represents the 95% CI. Risk ratios for Abu El-Asrar32 were calculated directly from raw data. An arrowhead means that the upper CI is not marked on the chart. The right-hand column indicates key adjustments. BP, blood pressure; DM, diabetes mellitus. *Studies which used only radiological diagnosis of infarct.

Retinopathy and stroke

Retinopathy was associated with incident stroke; among eight papers providing data (total population 25 354; 1019 strokes),8 11 14 15 19 20 22 44 the sRR for incident stroke in the presence versus absence of retinopathy was 2.1 (95% CI 1.7 to 2.6; fig. 1) with no significant heterogeneity between papers (χ2 p = 0.46).

Retinopathy was associated with prevalent stroke; among seven papers providing data (total population 8083; 979 strokes),9 10 16 17 29 32 33 the sRR for prevalent stroke in the presence versus absence of retinopathy was 2.45 (95% CI 1.4 to 4.3; fig. 2) with significant heterogeneity between papers (χ2 p = 0.003).

Arteriolar and venular widths and stroke

Decreased arteriolar width was not associated with incident stroke; among three papers providing data (total population 9262; 557 strokes),18 26 27 decreasing arteriolar width was not associated with incident stroke—sRR 0.9 (95% CI 0.8 to 1.1; fig. 1) with no significant heterogeneity between papers (χ2 p = 0.67)

Increased venular width was associated with incident stroke; among the same three papers providing data for this analysis (total population 9262; 557 strokes),18 26 27 venular widening was associated with incident stroke—sRR 1.4 (95% CI 1.1 to 1.7; fig. 1) with no significant heterogeneity between papers (χ2 p = 0.36). No papers assessed arteriolar or venular widths and prevalent stroke.

Six papers assessed the relationship between AVR and incident stroke, and we were able to extract data from five papers (21 717 subjects, 748 strokes).11 14 20 26 27 Decreased AVR was not associated with incident stroke (sRR 1.4, 95% CI 0.9 to 2.0; fig 1), although there was significant heterogeneity between these papers (χ2 p = 0.002). Three further papers found a significant association between decreased AVR and prevalent stroke (5451 subjects, 812 strokes)9 10 16 with sRR 1.2 (95% CI 1.1 to 1.3; fig. 2) with no significant heterogeneity between papers (χ2 p = 0.47), but another paper from which we could not extract data reported no association between AVR and prevalent stroke.23

Retinal arteriolar emboli or arteriolar occlusion and stroke

Six papers assessed retinal emboli and incident stroke12 21 24 36 40 42 from which we could extract data from four papers12 21 24 40 but Klein et al21 present 5-year follow-up data from the BDES, and Klein et al24 present a 10-year follow-up from the same patients; thus when combining studies, we excluded Klein et al.21 Furthermore, Wang et al12 presented a pooled analysis of data from the BDES and the BMES. As the data from the BDES are included in Klein et al,24 we present in this analysis the data presented in Wang et al12 from the BMES (to avoid double counting), so a total of 8720 subjects and 266 strokes gave an sRR of 2.9 (95% CI 1.6 to 5.1; fig. 1) with no significant heterogeneity between papers (χ2 p = 0.21). One paper assessed retinal artery occlusion and prevalent stroke, but we were unable to extract any data.36

Retinal vein occlusion and stroke

Two papers that investigated the association between RVO and incident stroke (9168 subjects; 359 strokes) found no association—sRR 1.2 (95% CI 0.8 to 1.9; fig. 1)28 41 with no significant heterogeneity between papers (χ2 p = 0.37). A further two papers studied RVO and prevalent stroke (4744 subjects, 43 strokes)—the one paper (3654 subjects) from which we could extract data found RVO was associated with prevalent strokes (sRR 3.8, 95% CI 1.9 to 7.6; fig. 2).25

Retinal microvascular abnormalities in ischaemic versus haemorrhagic stroke

No papers directly compared and reported differences between retinal microvascular abnormalities in ischaemic versus haemorrhagic stroke.

Retinal microvascular abnormalities and large artery versus small artery stroke

Only one pilot study34 directly compared retinal microvascular abnormalities in ischaemic stroke subtypes. The diagnosis of stroke and subtyping was based on clinical and radiological features at the time of the stroke. This small study (59 patients in total) found that there were similarly high levels of mild retinal arteriosclerosis (defined as altered central arteriolar light reflex) in lacunar stroke (prevalence 92%) and cortical stroke (prevalence 80%).

Retinal microvascular abnormalities and transient ischaemic attacks

No papers solely examined retinal disease and TIA. Two papers that included TIAs in their stroke outcomes did not present separate data for TIA.11 32


This review has demonstrated an association between retinal microvascular abnormalities and any stroke despite large variations in study design, stroke outcomes used and a lack of detail in diagnosing both the presence and type of stroke. It has shown that the presence of retinopathy is associated with incident stroke and prevalent stroke. Furthermore, incident stroke was associated with retinal artery embolism, venular widening and decreased AVR but not with arteriolar narrowing. There were no data on arteriolar narrowing or venular widening and prevalent stroke, and decreased AVR was not associated with prevalent stroke. Thus, there were some inconsistencies in results, which, combined with the heterogeneity between studies for some analyses, means that the data on different types of retinal microvascular abnormality and stroke should be viewed cautiously. No published studies have adequately compared retinal microvascular abnormalities in haemorrhagic versus ischaemic stroke or between different ischaemic stroke subtypes, so we are unable to answer one of our initial hypotheses that retinal microvascular abnormalities may shed light on the pathophysiology of small vessel disease.

Population-based studies contributed the majority of papers, and so the total number of subjects was large (62 975 subjects), but in healthy and relatively young populations (mean age 62.5 years, younger than the mean age of stroke onset in the UK of 72 years) stroke is rare, making it difficult to show differences between exposure groups. The rareness of strokes is reflected in wide 95% CI,15 the heterogeneity between studies for some associations and the occasional need to combine cohorts to demonstrate associations in some primary publications,12 indicating that the data on specific retinal features and stroke should be viewed with caution. Although the general association between any retinal microvascular abnormalities and stroke may be robust, the details of the associations between different retinal microvascular features and stroke are inconsistent and may be less reliable. Case control studies can be used when the outcome of interest is rare, but studies in this review used the general population as controls35 41 42 or MRI scans of patients who self-presented to hospital for general health checks.37 We were unable to perform any subanalysis by age group, but it is likely that older populations and populations where all patients have had a stroke will have more retinal abnormalities, thereby increasing the chance of finding associations between retinal changes and stroke subtypes.

The overall results of the review are promising in that for most analyses, the heterogeneity between studies is low. However, there are inconsistencies. For example, it is odd that decreased AVR should be associated with prevalent stroke but not incident stroke which could be due to the significant heterogeneity in the results from the incident stroke papers (perhaps resulting from Klein et al20 using patients with type I diabetes as their cohort who were younger than the patients in the other studies) possibly causing a type II statistical error for the association between decreased AVR and incident stroke or recall bias affecting the detection of prevalent stroke. Although there were a similar number of strokes, there were many more subjects in the analysis of incident stroke than of prevalent stroke and AVR. Similarly there is significant heterogeneity between the studies for retinopathy and prevalent stroke but not retinopathy and incident stroke, this may reflect the wide variety of methods used to identify and diagnose prevalent stroke as the number of strokes in the two analyses were similar. Some heterogeneity in the association between retinopathy and prevalent stroke may have resulted from Longstreth et al10 investigating associations with MRI defined infarcts in the Cardiovascular Health Study which involved an older age group (>65 years) than the others in the subgroup investigating retinopathy and prevalent stroke. Although we have not demonstrated much heterogeneity, it should be noted that with small numbers of outcomes, the power to detect heterogeneity is low, and our calculations may have missed a small degree of heterogeneity.

Stroke is difficult to diagnose. Ideally, the patient should be assessed by a suitably trained stroke expert at the time of the stroke and the diagnosis made with clinical features and appropriately timed brain imaging. Many of the incident stroke studies were population-based, and it is almost impossible for study investigators to assess every possible stroke. Accordingly, the diagnosis of stroke was made at either regular review when patients were asked if they had had a stroke in the preceding follow-up period and medical records reviewed, or patients were asked to report possible strokes to the investigators. Alternatively, discharge summaries or even death certificates were used to diagnose stroke. Although these techniques allow for the assessment of large numbers of subjects, they come at the price of reduced accuracy,4547 as discharge summaries and death certificates are often completed by inexperienced junior medical staff. To ascertain whether a patient had a history of stroke, many studies asked the patient and searched medical records. Although prone to recall bias, this approach is deemed acceptable for assessing prevalent stroke epidemiologically, but may be inadequate for detailed studies of pathophysiology. We have grouped together studies investigating certain retinal features and stroke outcomes but there were varying methods of both retinal assessment and more pertinently stroke assessment. We have tried to minimise this variation but it is a limitation of the results of this review that this variation exists.

In order to assess for a small study bias, we plotted a Funnel plot of the logarithm of the effect size and standard error of the effect size, and found that there was a paucity of small negative studies. Furthermore, the larger studies tended to have smaller effect sizes detailing the association between retinal microvascular abnormalities and stroke. One of the possible causes of this small study bias is publication bias, and it is likely that publication bias has affected the results of this review.

Stroke is a heterogeneous disorder. The two main pathophysiological divisions are haemorrhagic and ischaemic stroke, and ischaemic stroke is further subtyped into lacunar (or small vessel disease) and cortical (large artery atheroma and cardiac embolism subtypes). Furthermore, while stroke subtypes share many vascular risk factors, their underlying pathophysiology varies, reflecting different disease processes. Any study aiming to understand the pathophysiology of stroke, or even to assess novel risk factors for stroke, should subdivide stroke into ischaemic and haemorrhagic, and preferably further subtype ischaemic stroke. This requires careful clinical assessment and appropriate brain imaging. In the ARIC study, strokes were subtyped according to their likely cause based on clinical and imaging criteria where available—haemorrhagic or ischaemic with ischaemic strokes further subtyped into the important subdivisions of thrombotic or embolic stroke but not lacunar versus cortical. However, due to the small number of strokes (and especially haemorrhagic strokes)14 15 direct comparisons between stroke subtypes were not reported. The Rotterdam Study investigated cerebral infarction (but brain imaging was only available for 68% of patients).26 Other papers subtyped stroke but did not compare retinal microvascular abnormalities between the different stroke subtypes but only between any stroke and non-stroke controls.33 40 One study that did compare retinal microvascular abnormalities in lacunar and cortical stroke directly and had adequate diagnosis and subtyping34 was underpowered (n = 60) and used subjective measures of retinal arteriolar sclerosis (altered central light reflex).

MRI used appropriately is a powerful tool for diagnosing and subtyping stroke, but in the absence of clinical data about neurological deficits, the abnormal areas seen on imaging are difficult to interpret, especially as they are common in older populations.48 Lacunar infarcts can have considerable overlap with white-matter lesions. We excluded studies dealing solely with white-matter lesions, but some of the included studies may have misclassified white-matter lesions as lacunes and vice versa, thus introducing a small bias. Without detailed diffusion weighted MR imaging, or careful computed tomography assessment at the time of any symptoms, it is not clear which, if any, lacunes may have represented previous clinically evident stroke. As such, stroke remains a clinical diagnosis, and associations between retinal features and lacunes seen on MRI may be less reliable in aiding to understand the pathophysiology of stroke subtypes.

A few earlier studies used direct ophthalmoscopy to record retinal microvascular abnormalities. Apart from this, the quality of retinal assessment used in most papers was high. Almost all of the later studies, and certainly all of the large population-based cohort studies, used retinal photography with grading by trained analysts using internally and externally validated techniques blinded to other clinical information.

The strength of the associations between retinal microvascular abnormalities and stroke is encouraging, especially given the consistency between studies. This suggests that retinal examination offers an excellent way to study non-invasively the effects of common vascular risk factors on small vessels and possibly for gaining a better understanding of the pathophysiological processes involved in cerebral small vessel disease. The lack of studies which directly compare retinal microvascular abnormalities and stroke ischaemic subtypes is therefore disappointing, and further studies carefully characterising stroke subtype with comprehensive assessment at the time of the stroke are required if retinal microvascular abnormalities are to shed light on the pathophysiology of lacunar stroke. The paucity of strokes, lack of detailed stroke assessment and heterogeneity between studies for some associations mean that the data on individual retinal features, and stroke should be viewed with caution and underpins the need for more large, stroke-focussed, robust studies.


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  • Funding: FND is supported by the Wellcome Trust (grant number 075611), who had no role in the conception or writing of this review.

  • Competing interests: None.

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