Article Text

PDF
B38 Excessive Monoamine Oxidase A/b Activity Contributes To Stress-induced Neuronal Death In Huntington Disease
  1. J Ooi1,
  2. MR Hayden1,2,3,
  3. MA Pouladi1,3
  1. 1Translational Laboratory in Genetic Medicine, 8A Biomedical Grove, Immunos, Level 5, Singapore 138648, Singapore
  2. 2Centre for Molecular Medicine and Therapeutics, Child and Family Research Institute, University of British Columbia, Vancouver, BC V5Z 4H4, Canada
  3. 3Department of Medicine,Yong Loo Lin School of Medicine, National University of Singapore, 117597, Singapore

Abstract

Monoamine oxidases (MAO) are an important component of the homeostatic machinery that maintains the levels of monoamine neurotransmitters, including dopamine, in balance. Given the imbalance in dopamine levels observed in HD, the aim of this study was to examine MAO activity in cellular models of HD and in patient dermal fibroblasts. We show that cells expressing mutant HTT exhibit increased MAO expression and activity. Using cellular stress paradigms, we further demonstrate that the increase in MAO activity in mutant cells is accompanied by enhanced susceptibility to oxidative stress and cell death. Treatment of mutant cells with MAO inhibitors ameliorated oxidative stress and reduced cell death. This study demonstrates abnormal MAO expression and activity and suggests a potential use for MAO inhibitors in HD.

KeyWords
  • Huntington’s disease
  • monoamine oxidase
  • dopamine
  • striatal neuronal cells
  • neurotoxicity
  • monoamine oxidase inhibitors
  • therapeutics

Statistics from Altmetric.com

Request permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.