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B20 Dissecting the role of nucleolar stress in huntington’s disease
  1. Rosanna Parlato1,
  2. Aynur Soenmez1,
  3. Salome Tabea Spieth1,2,
  4. Rasem Mustafa1,2,
  5. Christian Litke2,
  6. Grzegorz Kreiner3,
  7. Holger Bierhoff4,
  8. Francesca Tuorto5,
  9. Jose Naranjo6,
  10. Birgit Liss1
  1. 1Institute of Applied Physiology, University of Ulm, Ulm, Germany
  2. 2Institute of Anatomy and Medical Cell Biology, University of Heidelberg, Heidelberg, Germany
  3. 3Department of Brain Biochemistry, Institute of Pharmacology, Polish Academy of Sciences, Cracow, Poland
  4. 4Department of Molecular Biology of the Cell II, DKFZ-ZMBH Alliance, German Cancer Research Centre, Heidelberg, Germany
  5. 5Division of Epigenetics, DKFZ-ZMBH Alliance, German Cancer Research Centre, Heidelberg, Germany
  6. 6National Biotechnology Centre, CSIC-CIBERNED, Madrid, Spain

Abstract

Background The expansion of CAG repeats in the mutant huntingtin (mHtt) protein impairs, among other fundamental cellular functions, the transcription of rRNA genes causing inhibition of rRNA synthesis and consequent disruption of nucleolar integrity, also known as nucleolar stress.

Aims We aim at dissecting the contribution of nucleolar stress to the onset and progression of HD.

Methods/techniques We mimicked for the first time nucleolar stress in striatal medium spiny neurons in mutant mice. This was achieved by conditional ablation of the nucleolar transcription factor TIF-IA gene, crucial for the recruitment of the RNA polymerase I to the rRNA promoters. Next we analysed behavioural, cellular and transcriptional changes in these mutants.

Results/outcome By this strategy we could specifically induce nucleolar stress-dependent signalling showing that it triggers pathophysiological and molecular similarities with HD. In particular we identified a neuroprotective response dependent on p53 activity, PTEN upregulation, mTOR downregulation and induction of autophagy at an early stage of nucleolar stress. Interestingly, we could find evidence of early compensatory mechanisms targeting nucleolar activity in HD cellular and mouse models. Moreover protein synthesis is altered by mHtt.

Conclusions These results allow not only to better understand mechanisms of HD but also to identify potential disease modifiers linked to altered nucleolar activity.

  • Nucleolus
  • compensation
  • stress

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