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C12 A new setup of the discrimination test as part of an assessment battery for phenotyping minipigs transgenic for huntington’s disease
  1. Lorena Rieke1,
  2. Sarah Schramke1,
  3. Tamara Matheis1,
  4. Robin Schubert1,
  5. Frauke Freisfeld1,2,
  6. Jan Motlik3,
  7. Verena Schuldenzucker1,4,
  8. Ralf Reilmann1,2,5
  1. 1George-Huntington-Institute, Technology Park Muenster, Muenster, Germany
  2. 2Department of Clinical Radiology, University of Muenster, Muenster, Germany
  3. 3Laboratory of Cell Regeneration and Plasticity, Institute of Animal Physiology and Genetics, Libechov, Czech Republic
  4. 4Institute of Zoology, University of Veterinary Medicine Hannover, Hannover, Germany
  5. 5Department of Neurodegenerative Diseases and Hertie-Institute for Clinical Brain Research, University of Tuebingen, Tuebingen, Germany

Abstract

Backround The Libechov tgHD minipig model is currently translated to first preclinical studies in HD. We here introduce a new semi-automated setup of the discrimination test as part of an assessment battery for phenotyping minipigs. The discrimination test primarily assesses cognitive dysfunction.

Objective To improve the feasibility and sensitivity of the discrimination test in minipigs.

Methods Thirty-two minipigs – 18 wt and 14 tg (with an N-terminal fragment of the huntingtin gene coding for 548 aa with 124Q) performed a colour discrimination test using a setup previously published. Based on experiences, a new semi-automated computer controlled setup was developed, which replaces the manual assessments with three identical boxes of different colour. The new setup permits displaying visual cues such as different colours but also more complex cues such as various shapes and figures and variation of exposure times. In addition, minipig behaviour may be assessed using computerised sensors.

Results The published discrimination test showed that both the wt and tgHD minipigs are able to differentiate between the three colours. Advantages and perspectives of the novel setup are presented.

Conclusion While the implementation of the published discrimination test is feasible and well tolerated, a novel semi-automated test will expand the cognitive features that can be tested, e.g. by using other colours, different shapes or exposure times.

Acknowledgement This study was funded by the CHDI Foundation.

  • animal models
  • minipig
  • HD
  • phenotyping
  • behavioural
  • preclinical research

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