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Detection of HP10 antigen in serum for diagnosis and follow-up of subarachnoidal and intraventricular human neurocysticercosis
  1. A Fleury (afleury{at}biomedicas.unam.mx)
  1. Instituto Nacional de Neurologia y Neurocitugia, Mexico
    1. M Hernandez (marysel_01{at}yahoo.com.mx)
    1. Instituto de Investigaciones Biomedicas, UNAM, Mexico
      1. M Avila (mallhely{at}hotmail.com)
      1. Instituto de Investigaciones Biomedicas, UNAM, Mexico
        1. G Cardenas (grace_goker{at}yahoo.de)
        1. Instituto Nacional de Neurologia y Neurocirugia, Mexico
          1. R J Bobes (rbobes{at}biomedicas.unam.mx)
          1. Instituto de Investigaciones Biomedicas, UNAM, Mexico
            1. M Huerta (mirnahuerta{at}yahoo.com)
            1. Facultad de Medicina, Benemérita Universidad Autónoma de Puebla, Mexico
              1. G Fragoso (gladis{at}servidor.unam.mx)
              1. Instituto de Investigaciones Biomedicas, UNAM, Mexico
                1. L Uribe-Campero
                1. Laboratorios Silanes, S.A. de C.V. de Mexico, Mexico
                  1. L J.S. Harrison (leslie.harrison{at}ed.ac.uk)
                  1. Division of Veterinary Clinical Sciences, incorporating Centre for Tropical Veterinary Medicine Roya, United Kingdom
                    1. R.M.E Parkhouse
                    1. Instituto Gulbenkian de Ciencia, Portugal
                      1. E Sciutto (edda{at}servidor.unam.mx)
                      1. Instituto de Investigaciones Biomedicas, UNAM, Mexico

                        Abstract

                        Introduction: Neurocysticercosis (NC), a parasitic disease caused by Taenia solium, may be either asymptomatic or show a mild to severe clinical picture with intracranial hypertension. The most severe form of the disease is caused when viable cysticerci are localized in the ventricles or in subarachnoidal cisterns at the base of the skull. Detection of the secreted metacestode antigen HP10 in cerebrospinal fluid is a sensitive and specific method for the diagnosis of these severe NC cases.

                        Objective & Methods: To evaluate the validity of HP10 antigen detection ELISA when applied to serum, using paired serum and cerebrospinal fluid samples from 116 radiologically and clinically characterized NC patients.

                        Results: The HP10 antigen assay exhibited a similarly high sensitivity to identify severe NC cases with sera (84.8%) as with CSF (91.3%). In contrast, HP10 antigen was rarely detected in asymptomatic or mild NC cases (3 of 57). Importantly, the HP10 antigen assay applied to serum revealed high specificity (94%) when used in 126 serum samples of non-NC subjects from an endemic community with confirmed coproparasitological diagnosis of intestinal parasitic infections. Finally, the HP10 assay also proved to be of value in the follow-up of treated patients.

                        Conclusion: This study confirms that detection of the metacestode HP10 antigen in serum is a useful tool for diagnosis and follow-up of patients with severe forms of neurocysticercosis treated with cysticidal drugs.

                        • HP10
                        • antigen detection
                        • diagnosis
                        • neurocysticercosis

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