Article Text


Research paper
Deep brain stimulation in the caudal zona incerta versus best medical treatment in patients with Parkinson’s disease: a randomised blinded evaluation
  1. Patric Blomstedt1,
  2. Rasmus Stenmark Persson1,
  3. Gun-Marie Hariz1,2,
  4. Jan Linder1,
  5. Anna Fredricks1,
  6. Björn Häggström1,
  7. Johanna Philipsson1,
  8. Lars Forsgren1,
  9. Marwan Hariz1,3
  1. 1 Department of Pharmacology and Clinical Neuroscience, Umeå University, Umeå, Sweden
  2. 2 Unit of Occupational Therapy, Department of Community Medicine and Rehabilitation, Umeå University, Umeå, Sweden
  3. 3 Unit of Functional Neurosurgery, UCL Institute of Neurology, London, UK
  1. Correspondence to Dr Patric Blomstedt, Department of Clinical Neuroscience, University Hospital of Umeå, Umeå 901 85, Sweden; patric.blomstedt{at}


Background Several open-label studies have shown good effect of deep brain stimulation (DBS) in the caudal zona incerta (cZi) on tremor, including parkinsonian tremor, and in some cases also a benefit on akinesia and axial symptoms. The aim of this study was to evaluate objectively the effect of cZi DBS in patients with Parkinson’s disease (PD).

Method 25 patients with PD were randomised to either cZi DBS or best medical treatment. The primary outcomes were differences between the groups in the motor scores of the Unified Parkinson’s Disease Rating Scale (UPDRS-III) rated single-blindly at 6 months and differences in the Parkinson’s Disease Questionnaire 39 items (PDQ-39). 19 patients, 10 in the medical arm and 9 in the DBS arm, fulfilled the study.

Results The DBS group had 41% better UPDRS-III scores off-medication on-stimulation compared with baseline, whereas the scores of the non-surgical patients off-medication were unchanged. In the on-medication condition, there were no differences between the groups, neither at baseline nor at 6 months. Subitems of the UPDRS-III showed a robust effect of cZi DBS on tremor. The PDQ-39 domains ‘stigma’ and ‘ADL’ improved only in the DBS group. The PDQ-39 summary index improved in both groups.

Conclusion This is the first randomised blinded evaluation of cZi DBS showing its efficacy on PD symptoms. The most striking effect was on tremor; however, the doses of dopaminergic medications could not be decreased. cZi DBS in PD may be an addition to existing established targets, enabling tailoring the surgery to the needs of the individual patient.

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Deep brain stimulation (DBS) is an established treatment for patients with Parkinson’s disease (PD) when pharmacological therapy alone does not provide sufficient relief or is associated with disabling side effects.1 2 At present, the subthalamic nucleus (STN) is the target of choice, but in some patients alternative targets, such as the ventral intermediate nucleus (VIM) of the thalamus or the globus pallidus internus (GPi), are preferred according to the symptomatic profile of PD or other patient-specific considerations.3 4 Recently, a number of studies have revived DBS in the posterior subthalamic area (PSA), a brain target that was commonly used for PD and tremors during the lesional era.5–9 The PSA is situated ventral to the VIM, between the red nucleus and the STN. This is a functionally crowded area that harbours the zona incerta including the caudal zona incerta (cZi), the Forel’s fields, the lemniscus and the prelemniscal radiations, among many other structures and pathways.10 11

Open-label studies have reported good results of DBS in various parts of the subthalamic area for different forms of tremor, including parkinsonian tremor.12–20 Furthermore, none of the studies above had reported on psychiatric side effects that resemble what had been described following STN DBS, such as hypomania, apathy, depression or suicide.21–25 One intriguing study compared in a non-randomised unblinded manner DBS in the STN with DBS in cZi and suggested the latter to be more effective, not only for tremor, but also for rigidity and bradykinesia.16

Hence, it was of interest to evaluate in a more objective manner the effects of DBS of the cZi in patients with PD who otherwise fulfilled the criteria for STN DBS in order to establish its potential efficacy and its safety profile. The aim of the present work was thus to evaluate the effects of bilateral cZi DBS versus best medical treatment in a single-blinded randomised manner in a group of patients with PD who were eligible for bilateral STN DBS. The two primary outcomes were (a) differences in off-medication scores of the motor part of the Unified Parkinson’s Disease Rating Scale (UPDRS-III) between medical and surgical groups at 6 months, and compared with their respective baseline scores, and (b) differences between the groups in quality of life rated on the 39 items of the Parkinson’s Disease Questionnaire 39 items (PDQ-39).

Materials and methods


Patients who would normally be considered for bilateral STN DBS were eligible for participation in this study. All patients had previously undergone a 123I FP CIT SPECT to support the diagnosis of PD. The inclusion criteria were thus the same as for STN DBS, that is, idiopathic PD, as diagnosed by a senior movement disorders neurologist according to the criteria of the UK Parkinson’s Disease Brain Bank26; L-dopa-responsive symptoms (defined as >30% improvement in motor scores of the UPDRS-III), disabling motor fluctuations with or without dyskinesias or disabling tremor; age <75 years; no relevant psychiatric comorbidities or cognitive decline; normal brain MRI and absence of surgical contraindications. All patients underwent a formal neuropsychological evaluation, a standardised L-dopa challenge and a brain MRI to rule out atrophy or other contraindications for brain surgery. All patients were informed about the aim of the study and the possibility of having DBS in the ‘established’ STN target if they chose not to participate in the study. Informed consent was obtained according to the Declaration of Helsinki.

Study design

Patients were randomised to either bilateral cZi DBS within 1 month from inclusion or to best medical treatment (and, if needed, bilateral cZi DBS after 6 months). UPDRS-III on-medication/off-medication was administered at baseline and at 6 months on-medication/off-medication and on-stimulation/off-stimulation by the same experienced evaluator (AF). The UPDRS-III scoring off-medication was done in the morning after withholding medication for 12 hours. The evaluation on-medication was done after administration of an L-dopa dose 50% higher than the patient’s normal morning dose. The evaluations at 6 months were conducted in both on-medication and off-medication states and on-stimulation/off-stimulation after the stimulator had been switched off/on for 60 min.

All UPDRS-III evaluations were videotaped with the patients wearing head caps. The video documentations of UPDRS-III of each patient comprised 4–6 segments (baseline on-medication/off-medication and 6 months on-medication/off-medication and on-stimulation/off-stimulation). The order of these segments was randomised and presented to two blinded experienced assessors (LF and MH) who were not involved in selection of patients, surgery, or follow-up, and who were unaware of the patient’s previous allocation. Two UPDRS items could not be evaluated on video: item 18 concerning speech and item 22 concerning rigidity. These two items from the unblinded UPDRS scoring were added to the blinded video-based scores of the motor UPDRS.


The surgical procedure has been described in detail previously.27 In summary, stereotactic implantation of the DBS electrodes 3389 (Medtronic, Minneapolis, Minnesota, USA) was performed in local anaesthesia using the Leksell frame model G (Elekta Instruments, Linköping, Sweden). The brain target was identified anatomically on stereotactic thin-slice T2-weighted axial MR images. The target point lay slightly posteromedial to the visualised posterior tail of the STN on the scan showing the maximal diameter of the red nucleus (figure 1). An intraoperative stereotactic CT scan was performed with the frame in place and fused with the preoperative stereotactic MRI for verification of the electrode position (figure 2). If the position of the lead did not coincide with the intended target, the electrode was repositioned and a new CT scan performed. The implantable pulse generator (Kinetra/Activa PC, Medtronic) was implanted in the same surgical session. All procedures were performed by the same surgeon (PB). Microelectrode recording was not performed.

Figure 1

The target point in the caudal zona incerta, medial to the tail of the subthalamic nucleus (STN). Rn, red nucleus.

Figure 2

Stereotactic intraoperative CT scan fused with stereotactic preoperative T2-weighted MRI scan demonstrating bilateral electrodes (arrows) implanted in the caudal zona incerta.

In the week after surgery, patients underwent in off-medication condition a systematic screening of electrode contacts to assess stimulation thresholds and effects on tremor, bradykinesia and rigidity, as well as stimulation-induced side effects. The contacts demonstrating the best effect in the absence of side effects were chosen for chronic stimulation. Stimulation settings and medications were evaluated and optimised during the study period, and eventual adverse events were recorded.

Outcome measures

The primary outcomes were the difference between groups at 6 months in the motor scores of the UPDRS-III and in health-related quality of life rated on the PDQ-39.

Secondary outcome measures included changes between baseline and 6 months in subscores of the UPDRS-III (tremor, speech, bradykinesia and axial subscores) in each group, as well as changes in dyskinesia and motor fluctuation scores according to UPDRS-IV, and in levodopa-equivalent daily doses (LEDD) between baseline and 6 months.


Patient demographic and clinical characteristics are presented as mean±SD and range. A two-tailed t-test was used for continuous variables. The Wilcoxon signed-rank test for paired samples was used for non-parametric data, to compare preoperative and postoperative scores within the medical and surgical group. Mann-Whitney U test was used for unpaired non-parametric data in order to compare scores between the medical and the surgical group. A P value of <0.05 was considered statistically significant.


Twenty-five patients met the inclusion criteria and accepted to be included in the trial. Of these 25 patients included and randomised, 6 could not or would not fulfil the trial: 4 patients (1 randomised to DBS and 3 to best medical management) quit directly after randomisation and did not wish to proceed. One patient randomised to DBS fell and broke her knee just before the surgery, and one patient refused to undergo evaluation with DBS turned off at 6 months. Hence, 19 patients (10 randomised to best medical management and 9 to DBS) fulfilled the trial and completed the evaluations at 6 months.

The patients’ demographic and clinical characteristics at baseline are presented in table 1: there were no significant differences between the groups at baseline. Both groups showed good response to the L-dopa test preoperatively.

Table 1

Patients’ characteristics in medical and surgical groups at baseline

Main outcomes

Table 2 shows the scores of UPDRS-III between groups at 6 months. The scores were significantly better for surgical patients on-stimulation off-medication compared with medical patients off-medication. In the on-medication condition, there were no significant differences in UPDRS scores between the two groups.

Table 2

Mean±SD of Unified Parkinson’s Disease Rating Scale scores at 6 months in medical and surgical groups, respectively

Table 3 shows the results of PDQ-39: the summary index was not different between medical and surgical groups, neither at baseline nor at 6 months. Compared with baseline, PDQ-39 summary index was improved in the surgical group by 36.4% and in the medical group by 24.4%. In the surgical group, the improvement was significant concerning subdomains ‘ADL’ and ‘stigma’, and in the medical group the improvement concerned only the summary index of the PDQ-39. At baseline, as well as at 6 months, the surgical group scored better than the medical group on the dimension ‘emotion’.

Table 3

Parkinson’s Disease Questionnaire 39 items (PDQ-39) at baseline and at 6 months in medical and surgical groups, respectively

Secondary outcomes

Table 4 shows the values of selected items of the UPDRS-III and values for LEDD and UPDRS-IV (dyskinesia). Tremor improved markedly and akinesia moderately in the surgical group on-stimulation off-medication compared with the medical group off-medication. There were no differences between the groups in speech, axial symptoms, dyskinesia or in LEDD. When comparing the scores at 6 months with the scores at baseline in each group, total UPDRS-III scores as well as scores of subitems tremor and akinesia still showed good improvement on-medication versus off-medication in both groups.

Table 4

Mean±SD scores of selected items of Unified Parkinson’s Disease Rating Scale (UPDRS-III), dyskinesia scores (UPDRS-IV) and levodopa-equivalent daily doses (LEDD), at baseline and at 6 months in medical group on-medication/off-medication, and in surgical group on-medication/off-medication on-stimulation/off-stimulation

When comparing UPDRS-III scores within the surgical group only, between on-stimulation and off-stimulation conditions at 6 months, there was a significant improvement mainly in tremor and moderately in akinesia.

Considering motor fluctuations, the majority of patients had mild fluctuations: at baseline, four patients in the surgical group and nine patients in the medical group reported predictable off periods (UPDRS-IV, item 36). Three patients in the surgical group and nine in the medical group reported unpredictable off periods (item 37). One patient reported sudden off periods in the medical group (item 38). The mean off-time score (item 39) was 0.9±0.9 in the surgical group and 1.6±0.8 in the medical group at baseline (P=NS). No statistically significant changes were seen within or between the groups at 6 months follow-up in any of the subitems 36–39.

Adverse events

In the surgical group, adverse events included a deep venous thrombosis in one patient 3 months after surgery that was treated successfully. No adverse events were encountered or reported in the medical group.

Stimulation parameters

Mean stimulation parameters at 6 months were 2.48±0.43 V; 152.2±10.3 Hz; 63.3±9.7 µs. Seven patients had monopolar and two bipolar stimulation. The most used contacts of the quadripolar electrode were the two middle contacts. No patient had the most proximal contact as a cathode. The active contacts (cathodes) for each individual patient with coordinates in relation to the midcommissural point are shown in figure 3. In relation to the visible posterior tail of the STN (pSTN), the active contacts were located at a mean of 1.9±1.1 mm medially, 0.6±0.5 mm posteriorly and 1.9±1.2 mm dorsally. The mean vectorial distance from the active contact to the pSTN was 3.1±0.8 mm. In relation to the midcommissural point, the active contacts were located 2.5±1.2 mm below, 7.0±0.4 mm posterior and 11.6±1.1 mm lateral.

Figure 3

Fused axial CT-MRI scans at the level of the active contact bilaterally in all operated patients. Coordinates are given in relation to the midcommissural point.


In this observer-blinded study on patients with advanced PD randomised to DBS in the cZi or to best medical management, it was shown that DBS in the cZi was safe and efficient. At 6 months, operated patients had 45% improvement of UPDRS-III scores off-medication on-stimulation compared with their off-stimulation condition, and 41% improvement compared with their baseline UPDRS-III scores. When compared with the non-surgical patients off-medication, the DBS-treated patients had 47% better UPDRS motor scores. There were no differences between the groups in the on-medication condition. The PDQ-39 summary index showed similar improvement in both groups at 6 months: the medical group improved by 24% and the DBS group by 36%.

Rationale for the study

Two large reviews of published papers on DBS of the PSA and cZi for various movement disorders19 20 have concurred in that this procedure seems to be quite efficient for tremor. One review hinted at the need for blinded evaluations,19 and the other review pointed to the need for quality-of-life assessments of patients.20 It is interesting to note that open-label studies that focused specifically on patients with PD undergoing cZi DBS reported a very positive effect on other symptoms than tremor: Carillo-Ruiz et al 14 evaluated five patients and reported that the UPDRS-III was improved by 65%, with a 90% improvement in tremor, 94% in rigidity and 75% in bradykinesia. In a non-randomised open-label sequential study, Plaha et al 16 compared the effects of DBS in the STN (17 electrodes) and the cZi (27 electrodes) and reported that the best effect was achieved in the cZi with a reduction of contralateral UPDRS-III score by 76%, tremor 93%, rigidity 76% and bradykinesia 65%. Nonetheless, according to the Movement Disorder Society task force on DBS for PD, only DBS targeting the STN or the GPi qualify as evidence-based procedures for PD.28 Hence, it was desirable to establish in an objective manner whether cZi DBS was efficient for PD and whether it was better than medication alone. It was also important to analyse its effect profile on the various symptoms of PD, and its impact on quality of life, in a similar way to what Deuschl et al did in their seminal publication from 2006 comparing STN DBS versus best medical management2 at 6-month follow-up. Therefore, we chose to include in this trial patients with PD who would otherwise qualify for STN DBS.

Effect profile of cZi DBS on PD symptoms

In analogy to most open-label reports,12 15 17 27 29 cZi DBS had a profound effect on tremor in our patients. There was a 92% improvement in tremor scores when comparing DBS on versus baseline, and 93.4% improvement when comparing DBS on versus off at 6 months. Indeed, this is not surprising since high-frequency stimulation targeting the PSA and especially the area of the cZi will affect the cerebellothalamic fibres which are here concentrated in a small area, forming a bottleneck, before dispersing into the VIM thalamus.30–36 This would also explain the relatively modest level of energy needed in our patients to obtain a satisfactory effect on tremor, when stimulating this area (2.5 V in average).

The off-medication akinesia items of UPDRS-III showed 32% improvement between DBS on and baseline, and 38% improvement between DBS on and DBS off. This degree of improvement is less than what has been reported in other open-label non-blinded studies of DBS in PSA and cZi,14 16 and underscores the importance of blinded clinical evaluations in surgery for movement disorders, especially when investigating ‘new’ brain targets for DBS. Interestingly, the level of improvement in our study was not much different from what was shown in similar randomised studies of bilateral STN DBS, where Deuschl et al 2 reported an improvement of 41% of UPDRS-III at 6 months in the German multicentre trial, and Williams et al 37 reported 36.2% improvement at 12 months in the British multicentre ‘PD SURG’ trial.

Finally, in our study, axial items and items related to speech and dyskinesia did not show any differences between groups, nor between on-stimulation and off-stimulation in the DBS group. Also, the LEDD were unchanged between groups and within groups, although there was a non-significant tendency for a reduction of LEDD in the surgical group. Hence, at variance with what is known from studies on STN DBS, our patients with cZi DBS remained on practically the same doses of dopaminergic medications at 6 months as before surgery.

Strength and limitations of our study

To the best of our knowledge, this is the first study on DBS in the cZi using a randomisation between a medical and a surgical group, and an observer-blinded evaluation. On the other hand, we are aware of the limited number of patients in this study: it was not straightforward to recruit participants because, since the patients had to be eligible for the established STN DBS procedures, several patients who were cleared for surgery and who were informed as per protocol that cZi DBS was an investigational target opted to have DBS in the more established STN target instead.

At baseline, disease duration was 4 years shorter on average in the surgical group than in the medical group, and the scores of UPDRS-III off-medication were lower. These differences were, however, not statistically significant, which could be due to the small sample size and/or large SD. This difference can also be explained by our non-stratified randomisation. Furthermore, at the 6-month follow-up, the differences in off-medication scores of UPDRS-III between groups had decreased and remained not significant.

Another limitation of our study is the relatively short follow-up of 6 months, but this was similar to the length of follow-up of the first randomised study comparing STN DBS to best medical management.2 Besides, it would be difficult to ethically justify withholding any surgery for longer than 6 months in patients with advanced disease who were eligible for surgery but who were in the medical arm.

The place of cZi DBS in surgery for PD

Our study confirmed a strong effect of DBS in the cZi on parkinsonian tremor and moderate effect on bradykinesia. Therefore, this brain target may be considered in tremor-dominant parkinsonian patients who for various reasons may not be eligible for bilateral STN or GPi DBS. DBS in cZi can also be performed unilaterally if needed. Concerning VIM versus cZi DBS, our experience27 38 39 and that of others34–36 is that cZi DBS can be more efficient and more effective than VIM DBS. Additionally, as demonstrated in this study, DBS in cZi does have an effect, although moderate, on bradykinesia. Finally, compared with thalamic VIM DBS, cZi is also a target with more closely related anatomical structures that are visible on MRI, thereby allowing direct anatomical targeting as shown in figures 1 and 2.

In our practice of DBS, the cZi has now replaced the VIM both for selected patients with PD as well as patients with other tremor disorders. The fact that PD medications are not decreased lends this target to be used for unilateral DBS in patients with asymmetric parkinsonism.


In this observer-blinded study on patients with advanced PD randomised to DBS in the cZi or to best medical management, it was shown that DBS in the cZi was efficient. The powerful effect of cZi DBS on tremor was confirmed. There was a modest, although significant, improvement on akinesia but not of the same magnitude as the improvement reported following STN DBS. Future reports will provide data on longer-term follow-up. Also, further studies are needed in order to determine the role of cZi DBS in the therapeutic armamentarium for PD, especially in relation to STN DBS. What can be stated so far is that the cZi as a target for DBS in PD will be an addition to the existing established targets, which may increase the possibility to tailor the surgery to the needs of the individual patients.


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  • LF and MH contributed equally.

  • Contributors PB, LF, MH and RSP designed the research. RSP, G-MH, JL, AF, BH, JP, LF, MH and PB conducted the study and acquired the data. RSP, G-MH, PB, MH and JL analysed and interpreted the data. RSP, PB and MH drafted the manuscript. All authors commented on and critically revised the manuscript. All authors approved the final version of the manuscript.

  • Funding This study was supported by a grant (ALF) from the University Hospital of Northern Sweden (Spjutspets), and the University of Umeå.

  • Competing interests PB is consultant for Medtronic and shareholder in Mithridaticum AB. JL has received honoraria for lectures from Lundbeck, AbbVie, IPSEN, Nordic InfuCare,, and Medtronic. MH has received honoraria and travel expenses from Medtronic and Boston Scientific for speaking at meetings. MH is supported by the Parkinson Appeal UK, and the Monument Trust.

  • Patient consent Obtained.

  • Ethics approval Ethical Committee of Umeå University.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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