Abstract
In vertebrate DMA, 3% to 5% of cytosine residues are present as 5–methylcytosine, and it is generally accepted that essentially all of this methylation occurs at cytosines which are contained in the symmetrical dinucleotide CpG. In this report we demonstrate, using bisulphite genomic sequencing, that the methylation machinery of mammalian cells is capable of both maintenance and de novo methylation at CpNpG sites. The existence of inherited CpNpG methylation in mammalian cells has important implications in gene regulation and in the aetiology of disease
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Clark, S., Harrison, J. & Frommer, M. CpNpG methylation in mammalian cells. Nat Genet 10, 20–27 (1995). https://doi.org/10.1038/ng0595-20
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DOI: https://doi.org/10.1038/ng0595-20
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