Abstract
Gender differences and the effect of chronic haloperidol on the rat brain dopamine transporter is reported. The density of striatal dopamine transporter sites labelled with [3H]GBR 12935, and of substantia nigra dopamine transporter mRNA measured by in situ hybridization were higher in female compared to male rats whereas striatal D2 specific binding labelled with [3H]spiperone was not significantly higher. Daily haloperidol treatment (1 mg/kg, i.p.) for 21 days increased striatal [3H]spiperone specific binding but left unchanged striatal [3H]GBR 12935 binding density and affinity as well as substantia nigra dopamine transporter mRNA levels. A reduce clearance rate of dopamine in the striatum after acute and chronic haloperidol was previously reported; the present results indicate that this may occur without changes in the sites of dopamine transport or in gene expression of this transporter.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Brain Chemistry / drug effects
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Brain Chemistry / physiology*
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Carrier Proteins / metabolism*
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Dopamine / metabolism*
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Dopamine Antagonists / pharmacology*
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Dopamine Plasma Membrane Transport Proteins
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Dopamine Uptake Inhibitors / pharmacology
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Female
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Haloperidol / pharmacology*
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In Situ Hybridization
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Male
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Membrane Glycoproteins*
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Membrane Transport Proteins*
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Neostriatum / drug effects
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Neostriatum / metabolism
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Nerve Tissue Proteins*
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Piperazines / pharmacology
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RNA, Messenger / biosynthesis
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Rats
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Rats, Sprague-Dawley
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Receptors, Dopamine D2 / drug effects
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Receptors, Dopamine D2 / metabolism
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Sex Characteristics
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Spiperone / metabolism
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Substantia Nigra / drug effects
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Substantia Nigra / metabolism
Substances
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Carrier Proteins
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Dopamine Antagonists
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Dopamine Plasma Membrane Transport Proteins
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Dopamine Uptake Inhibitors
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Membrane Glycoproteins
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Membrane Transport Proteins
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Nerve Tissue Proteins
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Piperazines
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RNA, Messenger
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Receptors, Dopamine D2
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Spiperone
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1-(2 (diphenylmethoxy)ethyl)-4-(3-phenylpropyl)piperazine
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Haloperidol
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Dopamine