Brain dopamine transporter: gender differences and effect of chronic haloperidol

Brain Res. 1995 Sep 18;692(1-2):269-72. doi: 10.1016/0006-8993(95)00611-s.

Abstract

Gender differences and the effect of chronic haloperidol on the rat brain dopamine transporter is reported. The density of striatal dopamine transporter sites labelled with [3H]GBR 12935, and of substantia nigra dopamine transporter mRNA measured by in situ hybridization were higher in female compared to male rats whereas striatal D2 specific binding labelled with [3H]spiperone was not significantly higher. Daily haloperidol treatment (1 mg/kg, i.p.) for 21 days increased striatal [3H]spiperone specific binding but left unchanged striatal [3H]GBR 12935 binding density and affinity as well as substantia nigra dopamine transporter mRNA levels. A reduce clearance rate of dopamine in the striatum after acute and chronic haloperidol was previously reported; the present results indicate that this may occur without changes in the sites of dopamine transport or in gene expression of this transporter.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain Chemistry / drug effects
  • Brain Chemistry / physiology*
  • Carrier Proteins / metabolism*
  • Dopamine / metabolism*
  • Dopamine Antagonists / pharmacology*
  • Dopamine Plasma Membrane Transport Proteins
  • Dopamine Uptake Inhibitors / pharmacology
  • Female
  • Haloperidol / pharmacology*
  • In Situ Hybridization
  • Male
  • Membrane Glycoproteins*
  • Membrane Transport Proteins*
  • Neostriatum / drug effects
  • Neostriatum / metabolism
  • Nerve Tissue Proteins*
  • Piperazines / pharmacology
  • RNA, Messenger / biosynthesis
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Dopamine D2 / drug effects
  • Receptors, Dopamine D2 / metabolism
  • Sex Characteristics
  • Spiperone / metabolism
  • Substantia Nigra / drug effects
  • Substantia Nigra / metabolism

Substances

  • Carrier Proteins
  • Dopamine Antagonists
  • Dopamine Plasma Membrane Transport Proteins
  • Dopamine Uptake Inhibitors
  • Membrane Glycoproteins
  • Membrane Transport Proteins
  • Nerve Tissue Proteins
  • Piperazines
  • RNA, Messenger
  • Receptors, Dopamine D2
  • Spiperone
  • 1-(2 (diphenylmethoxy)ethyl)-4-(3-phenylpropyl)piperazine
  • Haloperidol
  • Dopamine