Immunoprecipitation of muscarinic acetylcholine receptor subtypes using subtype specific antiserum
| Disease | [3H]QNB bound (fmol/mg) | Proportions of precipitated mAChR | |||
|---|---|---|---|---|---|
| m1 (%) | m2 (%) | m3 (%) | m4 (%) | ||
| Frontal cortex | |||||
| Controls (n=7) | 1289 (139) | 52.0 | 9.1 | 6.9 | 10.0 |
| DLBD (n=6) | 1128 (262)‡ | 50.5 | 8.4 | 9.82-150 | 9.2 |
| AD (n=11) | 1407 (181) | 51.0 | 9.4 | 9.6** | 9.4 |
| Temporal cortex | |||||
| Controls (n=7) | 1934 (188) | 53.2 | 9.1 | 7.3 | 10.6 |
| DLBD (n=7) | 1120 (95)** † | 58.12-150 ‡ | 8.5‡ | 8.2 | 6.8** |
| AD (n=11) | 1371 (298)** | 52.1 | 11.6** | 9.3 | 7.6** |
Data are mean (SD) data from a representative experiment. The density of mAChR was estimated as specific binding with [3H]QNB, and ranged from 800 to 2200 fmol/mg membrane protein. The proportions of specifically precipitated mAChR subtypes were estimated as the differences in the amounts of bound [3H]QNB precipitated with specific antiserum and those precipitated with non-immune serum.
↵2-150 p<0.05; **p<0.01 v controls; †p<0.05;‡<0.01 v AD.