Variant | Multiple sclerosis (%) | Controls (%) | Published control frequency (%) |
---|---|---|---|
*The primary PCR for this assay was relatively unreliable such that typing success rate was 90% for cases and 80% for controls. Both of the other assays had typing success rates of greater than 95%. The manufacturer’s standard reaction conditions were used for all reactions except the primary amplification of IBD8 where a lower annealing temperature of 50°C was used along with four additional PCR cycles. | |||
Primary PCR primers | |||
IBD8: ACCTTCAGATCACAGCAGCC and GCTCCCCCATACCTGAAC | |||
IBD12: AAGTCTGTAATGTAAAGCCA and CCCAGCTCCTCCCTCTTC | |||
IBD13: CTCACCATTGTATCTTCTTTTCC and GAATGTCAGAATCAGAAGGG | |||
Extension primers | |||
IBD8: TTTTTTTTTTTTTTCATCTGAGAAGGCCCTGCTC | |||
IBD12: TGGCCTTTTCAGATTCTGG | |||
IBD13: TTTTTTTGGTGTCATTCCTTTCAAGGG | |||
IBD8* | 54 (4.8) | 34 (6.2) | 4 |
IBD12 | 11 (0.9) | 6 (0.9) | 1 |
IBD13 | 28 (2.3) | 8 (1.2) | 2 |