Immunoprecipitation of muscarinic acetylcholine receptor subtypes using subtype specific antisera
| Proportions of precipitated mAchR (%) | |||||
|---|---|---|---|---|---|
| Disease | [3H]QNB bound (fmol/mg) | M1 | M2 | M3 | M4 |
| Data are mean (SD) data from a representative experiment. The density of mAchR was estimated as specific binding with [3H]QNB, and ranged from 800 to 2500 fmol/mg membrane protein. The proportions of specially precipitated mAchR subtypes were estimated as the differences in the amounts of bound [3H]QNB precipitated with specific antiserum and those precipitated with non-immune serum. | |||||
| *p<0.05, **p<0.01; *** p<0.001 v controls; †p<0.05, ††p<0.01, †††p<0.0015 v AD; ‡p<0.01, ‡‡p<0.001 v DLB. | |||||
| AD, Alzheimer’s disease; aPD, atypical Pick’s disease; DLB, dementia with Lewy bodies; mAchR, muscarinic acetylcholine receptors; QNB, [3H]quinuclidinyl benzilate. | |||||
| Frontal cortex | |||||
| Controls (n=9) | 1440 (414) | 50.6 | 9.6 | 6.5 | 10.0 |
| DLB (n=6) | 1128 (262) | 50.5 | 8.4 | 9.8* | 9.2 |
| AD (n=11) | 1407 (181) | 51.0 | 9.4 | 9.6** | 9.4 |
| aPD (n=5) | 1076 (157) | 51.4 | 11.0 | 6.6† | 8.4 |
| Temporal cortex | |||||
| Controls (n=9) | 1957 (210) | 53.3 | 9.0 | 7.3 | 10.4 |
| DLB (n=7) | 1120 (95)*** | 58.1 | 8.5 | 8.2 | 6.8** |
| AD (n=11) | 1371 (298)*** | 52.1 | 11.6*‡ | 9.3 | 7.6** |
| aPD (n=5) | 797 (194)***††† | 43.4**††‡‡ | 18.0***††‡‡ | 6.7 | 8.8 |