Primers used for amplification of the splice variants of TRAIL and TRAIL receptors
| Product name | Primer | Sequence 5’→3’ | Location | Product length (bp) |
|---|---|---|---|---|
| TRAIL-α | TRAIL-fwd1 | TTCTACAGTTCAAGAAAAGC | Exons 3–4 joint | 145 |
| TRAIL-rev1 | GCCCAGAGCCTTTTCATTCT | Exons 4–5 joint | ||
| TRAIL-β | TRAIL-fwd2 | AGCTCGTTAGAAAGAAAAGC | Exons 2–4 joint | 195 |
| TRAIL-rev2 | CTCAGGAATGAATGCCCACT | Exon | ||
| TRAIL-γ | TRAIL-fwd3 | ACGAGCTGAAGCAGAAAAGC | Exons 1–4 joint | 145 |
| TRAIL-rev1 | GCCCAGAGCCTTTTCATTCT | Exons 4–5 joint | ||
| TRAILR-1 | TRAILR1-fwd | ACCAGGAACACAGCATGTCA | Exon | 132 |
| TRAILR1-rev | GTCACTCCAGGGCGTACAAT | Exon | ||
| TRAILR-2 isoform 1 | TRAILR2-fwd | CAGTGCGAAGAAGGCACCTT | Exon 4 | 145 |
| TRAILR2-rev1 | CCCCACTGTGCTTTGTACCT | 87 bp insertion in exon 5 | ||
| TRAILR-2 isoform 2 | TRAILR2-fwd | CAGTGCGAAGAAGGCACCTT | Exon 4 | 157 |
| TRAILR2-rev2 | TGATGCCTGATTCTTTGTGG | Exon 5 | ||
| TRAILR-3 | TRAILR3-fwd | TGCACAGAGGGTGTGGATTA | Exon | 108 |
| TRAILR3-rev | GGTCATGGTGCAGGAACTTT | Exon | ||
| TRAILR-4 α | TRAILR4-fwd1 | CAATTTGCCTTCTTGCCTGC | Exon 3 | 164 |
| TRAILR4-rev | CTCTGGGACACCCTGTTCTA | Exons 4–5 joint | ||
| TRAILR-4 β | TRAILR4-fwd2 | GGAGTGTCCAGCAGGTCAAA | Exons 2–4 joint | 137 |
| TRAILR4-rev | CTCTGGGACACCCTGTTCTA | Exons 4–5 joint | ||
| GADPH | GADPH-fwd | GAAGATGGTGATGGGATTTC | Exon | 225 |
| GADPH-rev | GAAGGTGAAGGTCGGAGT | Exon |
TRAIL, Tumour necrosis factor-Related Apoptosis Inducing Ligand.