Regular Article
Detection of Apolipoprotein E/Dimeric Soluble Amyloid β Complexes in Alzheimer's Disease Brain Supernatants

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Abstract

The inheritance of the apolipoprotein (apo) E4 allele is an important risk factor for late-onset Alzheimer's disease (AD). A major component of the Alzheimer's disease neuritic plaques is amyloid β (Aβ). We previously identified apoE/Aβ complexes within neuritic plaques (1). It was not known if this interaction takes place before or after Aβ peptides become incorporated into neuritic plaques. To address this question we sought evidence of apoE complexes with brain soluble Aβ peptides in AD and control patients. In addition, numerous proteins have been shown to bind Aβ peptidesin vitro.It is not know if any of these bind brain sAβin vivo.We found evidence for the presence of apoE/dimeric sAβ complexes in the AD brain and could not detect complexes with other Aβ peptide binding proteins. The binding of sAβ to apoE may be one factor influencing its clearance from the brain and/or its conformational state.

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1

Correspondence should be addressed to New York University Medical Center, Department of Neurology, 550 First Avenue, TH 427, New York, NY 10016. Fax: (212) 263-6751. E-mail: thomas. [email protected].