Summary
Despite enormous efforts to find a specific laboratory test for multiple sclerosis, agar gel electrophoresis of the CSF proteins has remained the next to the best one. This study presents evidence that thin layer polyacrylamide isoelectric focusing of the CSF gamma globulins is by far superior for this purpose. In effect, about 91% of the 262 multiple sclerosis patients studied had oligoclonal fractions present in the very alkaline region of the pH gradient. Of the same group of patients, only 65% did show pathological results when studied by agar gel electrophoresis. Of the 272 CSF samples from patients suffering from other neurological diseases, only about 7% showed the presence of oligoclonal bands in the same region of the pH gradient, when submitted to isoelectric focusing.
Zusammenfassung
Unter den verschiedenen Laboratoriumstesten für multiple Sklerose war bisher die Agargel-Elektrophorese der Liquoreiweißfraktionen der Test mit dem höchsten diagnostischen Wert. In der vorliegenden Studie wird nachgewiesen, daß die isoelektrische Fokalisation der Gammaglobuline mittels Dünnschichtpolyacrylamidgel bei Multipler Sklerose weitaus wertvollere Ergebnisse zeitigt. Es ließen sich bei 91% von 262 Multiple-Sklerose-Patienten im alkalischen Bereich des pH-Gradienten oligoklonale Fraktionen im Liquor nachweisen. Bei der gleichen Patienten-Gruppe zeigten bei der Agargel-Elektrophorese nur 65% pathologische Ergebnisse. Unter 272 Liquorproben von Patienten, die an anderen neurologischen Erkrankungen litten, war nur bei 7% das Vorhandensein von oligoklonalen Zonen in der gleichen Region der pH-Gradienten bei isoelektrischem Fokusieren nachweisbar.
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Delmotte, P., Gonsette, R. Biochemical findings in multiple sclerosis. J. Neurol. 215, 27–37 (1977). https://doi.org/10.1007/BF00312547
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DOI: https://doi.org/10.1007/BF00312547