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Knockdown of transmembrane protein 132A by RNA interference facilitates serum starvation-induced cell death in Neuro2a cells

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Abstract

Transmembrane protein 132A (TMEM132A) is a novel GRP78 binding protein that we recently discovered. However, the biological functions of TMEM132A are merely characterized because it does not encode any known structural domains. In this study, we down regulated intrinsic TMEM132A by RNA interference and identified a variety of genes that fluctuated during TMEM132A gene silencing using microarray analysis. TMEM132A-knockdown in Neuro2a cells caused neurite-like projection without any stimuli and enhanced the expression of ATF6 mRNA, an ER stress transducer, and GADD153 mRNA, a stress inducible gene. Under serum-deprived condition, TMEM132A-knockdown cells gradually retarded neurite-like projection and decreased cell viability. Moreover, TMEM132A knockdown markedly induced GADD153 expression due to serum starvation without affecting the level of cleaved caspase-3. Our data suggest that TMEM132A is an important factor of cell survival in regulating certain ER stress-related gene expression in neuronal cells.

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Abbreviations

ATF6:

Activating transcription factor 6

GADD153:

Growth arrest and DNA damage-inducible protein 153

GRP78:

Glucose-regulated protein, 78 kDa

siRNA:

Small interference RNA

TMEM132A:

Transmembrane protein 132A

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Acknowledgments

This study was supported in part by grant-in-aid for young scientists (B) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Department of Biomolecular Science, Faculty of Engineering, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan

    Kentaro Oh-hashi, Yoko Hirata & Kazutoshi Kiuchi

  2. Kazusa DNA Research Institute, 2-6-7 Kazusa-Kamatari, Kisarazu, Chiba, 292-0818, Japan

    Kazuhide Imai & Hisashi Koga

Authors
  1. Kentaro Oh-hashi
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  2. Kazuhide Imai
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  3. Hisashi Koga
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  4. Yoko Hirata
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Correspondence to Kentaro Oh-hashi.

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Oh-hashi, K., Imai, K., Koga, H. et al. Knockdown of transmembrane protein 132A by RNA interference facilitates serum starvation-induced cell death in Neuro2a cells. Mol Cell Biochem 342, 117–123 (2010). https://doi.org/10.1007/s11010-010-0475-9

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  • DOI: https://doi.org/10.1007/s11010-010-0475-9

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