Elsevier

Brain Research

Volume 208, Issue 1, 9 March 1981, Pages 35-58
Brain Research

Quantitative capillary topography and blood flow in the cerebral cortex of cats: an in vivo microscopic study

https://doi.org/10.1016/0006-8993(81)90619-3Get rights and content

Abstract

In 50 anesthetized cats the microcirculation in intermediate and deeper layers of the cerebral cortex was visualized in vivo by microtransillumination, and documented by high-speed microcinephotography. The viability of the preparation was verified in a series of experiment s demonstrating spontaneous vasomotion and responsiveness to chemical stimulation of pial arterioles and small arteries. Stereological methods for quantitative analysis of projected images of capillaries in a comparatively large tissue volume were employed to determine morphometric and topographical parameters of the asymmetric, highly tortuous intracortical capillary network. Capillary diameters (5.1 ± 0.84μm), radii of curvature (median 57 μm), total capillary lengths per tissue volume939 ± 338.2 mm/cu.mm, capillary volume fractions (2.1 ± 0.51%), total capillary surface areas per tissue volume (15.3 ± 4.85 sq.mm/cu.mm), and intercapillary distances (median 24.2 μm) showed significant interregional differences. The frequency distribution of the lenghts of capillary segments (median 108 μm) was best described by a Weibull distribution. On the average 90% of all capillaries were continously perfused. Capillary red cell flow (median velocity 1500 μm/sec) was predominantly unidirectional and conspicuously irregular. The variance of capillary red cell velocities (CRCVs) was significantly correlated (τ = 0.48) with capillary tortuosity. An extreme value distribution best describes the observed frequency distribution of CRCVs. Flow irregularities represented both twice noise and a significant stochastic periodicity at frequencies between 40 and 90 Hz.

Reference (59)

  • MyrhageR. et al.

    The microvascular bed and capillary surface area in rat extensor hallucis proprius muscle (EHP)

    Microvasc. Res.

    (1976)
  • RosenblumW.I. et al.

    Platelet aggregation and vasoconstriction in undamaged microvessels on cerebral surface adjacent to brain traumatized by a penetrating needle

    Microvasc. Res.

    (1978)
  • RosenblumW.I.

    Effect of pial arteriolar contriction on red cell velocity in pial venules and on venular diameter

    Microvasc. Res.

    (1975)
  • StarrM.C. et al.

    In vivo cellular and plasma velocities in microvessels of the cat mesentery

    Microvasc. Res.

    (1975)
  • TillichG. et al.

    Studies of the coronary microcirculation of the cat

    Amer. J. Cardiol.

    (1971)
  • WiederholdK.-A. et al.

    Three-dimensional reconstruction of brain capillaries from frozen serial sections

    Microvasc. Res.

    (1976)
  • AlexanderL. et al.

    Pathological alterations of cerebral vascular patterns

    Res. Publ. Ass. nerv. ment. Dis.

    (1938)
  • Ba¨rT. et al.

    Quantitative Beziehungen zwischen der Verzweigungsdichte und La¨nge von Kapillaren im Neocortex der Ratte wa¨hrend der postnatalen Entwicklung

    Z. Anat. Entwickl.-Gesch.

    (1973)
  • BaezS.

    Skeletal muscle and gastrointestinal microvascular morphology

  • BurtonK.S. et al.

    Reactive hyperemia in individual capillaries of skeletal muscle

    Amer. J. Physiol.

    (1972)
  • CampbellA.C.P.

    Variation in vascularity and oxidase content in different regions of the brain of the cat

    Arch. Neurol. Psychiat. (Chic.)

    (1939)
  • CraigieE.H.

    The comparative anatomy and embryology of the capillary bed of the central nervous system

    Res. Publ. Ass. nerv. ment. Dis.

    (1938)
  • DunningH.S. et al.

    The relative vascularity of various parts of the central and peripheral nervous system of the cat and its relation to function

    J. comp. Neurol.

    (1937)
  • EdvinsonL. et al.

    Effect of a calcium antagonist on experimental constriction of human brain vessels

    Surg. Neurol.

    (1979)
  • EkeA. et al.

    Induced hemodilution detected by reflectometry for measuring microregional blood flow and blood volume in cat brain cortex

    Amer. J. Physiol.

    (1979)
  • ErikssonE. et al.

    Microvascular dimensions and blood flow in skeletal muscle

    Acta physiol. scand.

    (1972)
  • FeldsteinM.L. et al.

    Frequency analysis of coronary intercapillary distances: site of capillary control.

    Amer. J. Physiol.

    (1978)
  • FitzgeraldJ.M.

    Implications of a theory of erythrocyte motion in narrow capillaries

    J. appl. Physiol.

    (1969)
  • GinsbergM.D. et al.

    Local glucose utilization in acute focal cerebral ischemia: local dysmetabolism and diaschisis

    Neurology (Minneap.)

    (1977)
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