Adhesion molecules on murine brain microvascular endothelial cells: expression and regulation of ICAM-1 and Lgp 55

doi:10.1016/0165-5728(92)90026-H

Journal of Neuroimmunology

Volume 36, Issue 1, January 1992, Pages 1-11

https://doi.org/10.1016/0165-5728(92)90026-H Get rights and content

Abstract

The mechanisms for the initiation of immune reactions in the central nervous system are poorly understood. In this report, we describe the presence of intercellular adhesion molecule-1 (ICAM-1) and Lgp 55 (suggested mouse homologue of human intercellular adhesion molecule-2, ICAM-2) on the surface of brain microvessel endothelium (EN) cells and show in vitro induction of ICAM-1 molecules on EN cells with pro-inflammatory cytokines. ICAM-1 expression was detected using flow cytometry analysis with biotinylated anti-ICAM-1 antibody (YN1/1.7.4). Lgp 55 expression was characterized using PA3 monoclonal antibody. According to our results, 30–40% of the non-activated brain EN cells expressed ICAM-1 and 15–20% expressed Lgp 55 molecules. The ICAM-1 molecule expression was increased after the activation of the cells with recombinant murine gamma interferon (IFN-γ), tumor necrosis factor (TNF-α), and interleukin-1α (IL1-α) in a dose-dependent manner. The increased ICAM-1 expression was detected as early as 2 h following the cytokine treatment and reached its maximum after 24 h. Transforming growth factor-β (TGF-β) did not influence the expression of ICAM-1 molecule. Lgp 55 molecule does not seem to be regulated by pro-inflammatory cytokines. ICAM-1 and Lgp 55 expression was found to be polarized on the luminal surface of EN by confocal laser microscopy suggesting accessibility for leukocytes. Inducible ICAM-1 expression may play a critical role in formation of inflammatory reactions inside the central nervous system.

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^☆: Supported by NIH grants NS24261, HL31944, HL14230 (Arteriosclerosis Center of Research) and by Veterans' Administration research funds.

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Adhesion molecules on murine brain microvascular endothelial cells: expression and regulation of ICAM-1 and Lgp 55☆

Abstract

J. Am Acad. Dermatol.

Mol. Immunol.

Blood

J. Am. Acad. Dermatol.

Trends Neurosci.

J. Neuroimmunol.

Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses

Identification of an inducible endothelial-leukocyte adhesion molecule

Upregulation and coexpression of adhesion molecules correlate with relapsing autoimmune demyelination in the central nervous system

J. Exp. Med.

Homing to central nervous system vasculature by antigen-specific lymphocytes. I. Localization of 14C-labeled cells during acute, chronic, and relapsing experimental allergic encephalomyelitis

Lab. Invest.

Cerebral microvessels and derived cells in tissue culture. II. Establishment, identification, and preliminary characterization of an endothelial cell line

In Vitro

Lymphcyte function-associated antigen-1 (LFA-1) interaction with intercellular adhesion molecule-1 (ICAM-1) is one of at least three mechanisms of lymphocyte adhesion to cultured endothelial cells

J. Cell. Biol.

Adhesion of T lymphoblasts to epidermal keratinocytes is regulated by interferon γ and is mediated by intercellular adhesion molecule 1 (ICAM-1)

J. Exp. Med.

CD4+ T cell subsets are differentially activated when the antigen is presented by murine brain capillary endothelium vs smooth muscle cells

FASEB J.

Endothelial adhesiveness for blood neutropils is inhibited by transforming growth factor-β

Science

Identification of a new cell surface glycoprotein with accessory function in murine T cell responses

J. Immunol.

Gamma interferon induces different keratinocyte cellular patterns of expression of HLA-DR and DQ and intercellular adhesion molecule-1 (ICAM-1) antigens

Br. J. Dermatol.

VLA proteins in the integrin family: structures, functions and their role on leukocytes

Annu. Rev. Immunol.

Leukocyte Integrins

Homing to central nervous system vasculature by antigen-specific lymphocytes. I. Localization of ¹⁴C-labeled cells during acute, chronic, and relapsing experimental allergic encephalomyelitis

CD4⁺ T cell subsets are differentially activated when the antigen is presented by murine brain capillary endothelium vs smooth muscle cells