Assessment of T-Cell receptor β-chain diversity by heteroduplex analysis
References (51)
- et al.
T cell receptor gene rearrangements define a monoclonal T cell proliferation in patients with T cell lymphocytosis and cytopenia
Blood
(1986) - et al.
Analysis of T-cell receptor beta chain (Tβ) gene rearrangements demonstrates the monoclonal nature of T-cell chronic lymphoproliferative disorders
Blood
(1986) Clonal diseases of large granular lymphocytes
Blood
(1993)- et al.
An alternative method for T-cell receptor repertoire analysis: clustering of human Vβ subfamilies selected in responses to staphylococcal enterotoxins B and E
Mol Immunol
(1993) - et al.
A single universal primer for the T-cell receptor (TCR) variable genes enables enzymatic amplification and direct sequencing of TCRβ cDNA of various T-cell clones
Hum Immunol
(1993) - et al.
A consensus primer to amplify both ct and β chains of the human T cell receptor
J Immunol Methods
(1994) - et al.
Analysis of rearranged T-cell receptor β-chain genes by polymerase chain reaction (PCR) DNA sequencing and automated high resolution PCR fragment analysis
Blood
(1995) - et al.
An alternative approach to the assessment of 78 T-cell clonality in celiac disease intestinal lesions through cDNA heteroduplex analysis of T-cell receptor VJ junctions
Hum Immunol
(1994) - et al.
Evidence for antigenic selection of large granular lymphocytes in a patient with Wiskott-Aldrich syndrome
Blood
(1995) - et al.
PCR-fingerprinting for selection of HLA matched unrelated marrow donors
Lancet
(1991)
Subgrouping of DR4 alleles by DNA heteroduplex analysis
Hum Immunol
(1992)
Biologic and clinical heterogeneity of lymphoproliferative diseases of peripheral mature T lymphocytes
Lab Invest
(1992)
Polymerase chain reaction amplification analyses of clonality in T-cell malignancy including peripheral T-cell lymphoma
Leukemia
(1994)
Major expansion of CD8+ T cells with a predominant Vβ usage during the primary immune response to HIV
Nature
(1994)
Oligoclonal T cell receptor gene rearrangements in blood lymphocytes of patients with acute Epstein-Barr virus-induced infectious mononucleosis
J Clin Invest
(1990)
Detection of human T lymphotrophic virus type I (HTLV-I) proviral DNA and analysis of the T cell receptor Vβ CDR3 sequences in spinal cord lesions of HTLV-I-associated myelopathy/tropical spastic paraparesis
J Exp Med
(1994)
Dominant T-cell-receptor β chain variable region Vβl4+ clones in juvenile rheumatoid arthritis
Selection for T-cell receptor Vβ-Dβ-Jβ gene rearrangements with specificity for a myelin basic protein peptide in brain lesions of multiple sclerosis
Nature
(1993)
The T-cell receptor Vβ gene usage in tumorinfiltrating lymphocytes and blood of patients with hepatocellular carcinoma
Cancer Res
(1992)
An analysis of T-cell-receptor variable-region genes in tumor-infiltrating lymphocytes within malignant tumors
Int J Cancer
(1991)
Predominant TCR-αβ variable and joining gene expression by muscle-infiltrating lymphocytes in the idiopathic inflammatory myopathies
J Immunol
(1994)
Selective activation and accumulation of oligoclonal Vβ-specific T cells in active pulmonary sarcoidosis
J Clin Invest
(1994)
Analysis of T cell receptor repertoire of muscle-infiltrating T lymphocytes in polymyositis. Restricted Vα/β rearrangements may indicate antigen-driven selection
J Clin Invest
(1993)
Clonal predominance of T cell receptors within the CD8+ CD45RO+ subset in normal human subjects
J Immunol
(1993)
Clonal populations of T cells in normal elderly humans: the T cell equivalent to “benign monoclonal gammapathy.”
J Exp Med
(1994)
Cited by (25)
Simultaneous quantification of recent thymic T-cell and bone marrow B-cell emigrants in patients with primary immunodeficiency undergone to stem cell transplantation
2010, Clinical ImmunologyCitation Excerpt :Each experimental procedure was accepted only if the standard curve slopes ranged between −3.55 and −3.30, PCR efficiencies varied from 91% to 100%, and correlation coefficients were higher than 0.998. TR beta variable (TRBV) chains repertoire was analyzed, after RNA isolation and cDNA synthesis, by PCR followed by heteroduplex analysis [22]. In this assay denatured and renatured amplified products of TRBV chains migrate in polyacrylamide gels as smears in case of a polyclonal repertoire, whereas heteroduplex or homoduplex bands indicate oligoclonal or clonal conditions.
Estimate of the total number of CD8+ clonal expansions in healthy adults using a new DNA heteroduplex-tracking assay for CDR3 repertoire analysis
2003, Journal of Immunological MethodsFrequency of clonally expanded T cells evaluated by PCR from a single cell
1999, Journal of Immunological Methods
Copyright © 1996 Published by Elsevier Inc.