Short communicationDideoxy fingerprinting (ddF): A rapid and efficient screen for the presence of mutations
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Cited by (185)
Functional and molecular evaluation of lymphocytes
2004, Journal of Allergy and Clinical ImmunologyCitation Excerpt :The gene defects responsible for many primary immunodeficiency diseases have been identified (Table III).65 Traditionally, screening methods, including single-strand conformational polymorphism or dideoxy fingerprinting, have been used to initially evaluate for mutations associated with immune deficiencies.66-68 However, the availability of fluorescence-based sequencing with automated multichannel capillary sequencers makes direct sequencing practical for mutation analysis in a number of immune deficiencies (Fig 3).69
Mutation analysis of the Sonic hedgehog promoter and putative enhancer elements in Parkinson's disease patients
2004, Molecular Brain ResearchChapter 12 Pharmacogenomics: Methodologies for genotyping and phenotyping
2004, Handbook of Analytical SeparationsCitation Excerpt :If the DNA contains a variant (single base modification, insertion, or deletion), it generally forms a different conformer that migrates differently when compared to wild-type DNA [24]. The sensitivity of this method can be increased to 100% by coupling it with restriction enzyme fingerprinting [25] or dideoxy-sequencing fingerprinting [26]. dHPLC detects polymorphisms by analyzing the DNA mobility of different heteroduplexes using chromatography under slightly denaturing conditions.
Isoform Heterogeneity of the Human Gephyrin Gene (GPHN), Binding Domains to the Glycine Receptor, and Mutation Analysis in Hyperekplexia
2003, Journal of Biological ChemistryCitation Excerpt :Bidirectional Dideoxy Fingerprinting (DDF) Analysis—DDF analysis was carried out using [α-33P]ddNTP/dNTP mixtures and thermosequenase reactions (Amersham Biosciences). DDF patterns for GPHN assays were resolved on MDE nondenaturing gels (48, 49). dHPLC—Analysis was carried out using the Transgenomics dHPLC 2100 WAVER DNA Fragment Analysis System and DNASepR column (Transgenomic, Santa Clara, CA).