Protein zero of peripheral nerve myelin: Biosynthesis, membrane insertion, and evidence for homotypic interaction

doi:10.1016/0896-6273(90)90057-M

Neuron

Volume 4, Issue 3, March 1990, Pages 449-460

https://doi.org/10.1016/0896-6273(90)90057-M Get rights and content

Abstract

Protein zero (P₀), an integral membrane glycoprotein synthesized by Schwann cells, is the major glycoprotein of peripheral nerve myelin. The predicted disposition of P₀ with respect to the membrane bilayer postulates the existence of extracellular and intracellular domains, that mediate compaction of the myelin lamellae. We used in vitro translations programed with sciatic nerve mRNA and cells transfected with a P₀ cDNA construct to study the biosynthesis and topology of P₀ in the bilayer. The behavior of P₀ at the cell surface, when expressed under physiological conditions, was also examined. We have verified the topological predictions of an earlier model, derived from analysis of a P₀ cDNA, and provide evidence that the extracellular domain of P₀ mediates homotypically cell-cell interactions in the transfectants.

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Neuron

ArticleProtein zero of peripheral nerve myelin: Biosynthesis, membrane insertion, and evidence for homotypic interaction

Abstract

J. Biol. Chem.

Cell

Cell

Neuron

Cell

J. Biol. Chem.

J. Cell Biol.

Dev. Biol.

Neurochem. Int.

Immunol. Today

Membrane domains of intestinal epithelial cells: distribution of Na+K+, ATPase and the membrane skeleton in adult rat intestine during fetal development and after epithelial isolation

J. Cell Biol.

Purification of biologically active globin messenger RNA by chromatography on oligothymidilic acid-cellulose

Identification of the major proteins that promote neuronal process outgrowth on Schwann cells in vitro

J. Cell Biol.

Intracellular protein topogenesis

The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent

Biochem. J.

Molecular organization of myelin

Synthesis and incorporation of myelin polypeptides into CNS myelin

J. Cell Biol.

Cell adhesion molecules in the regulation of neuronal form and tissue pattern

Annu. Rev. Cell Biol.

Biogenesis of peroxisomes: intracellular site of synthesis of catalase and uricase

Expression of recombinant plasmids in mammalian cells is enhanced by sodium butyrate

Nucl. Acids Res.

Cloning and expression of cDNA encoding a neural calcium-dependent cell adhesion molecule; its identity in the cadherin gene family

J. Cell Biol.

The P0 glycoprotein of peripheral nerve myelin

Can. J. Biochem.

Compact myelin exists in the absence of basic protein in the shiverer mutant mouse

Nature

Two forms of 113236/myelin-associated glycoprotein, a cell adhesion mole cule for postnatal neural development, are produced by alternative splicing

Neural protein 113236/myelin-associated glycoprotein (MAG) defines a subgroup of the immunoglobulin superfamily

Immunol. Rev.

Cell adhesion molecules in neural development

Dev. Neurosci.

Article
Protein zero of peripheral nerve myelin: Biosynthesis, membrane insertion, and evidence for homotypic interaction

Membrane domains of intestinal epithelial cells: distribution of Na⁺K⁺, ATPase and the membrane skeleton in adult rat intestine during fetal development and after epithelial isolation

The labelling of proteins to high specific radioactivities by conjugation to a ¹²⁵I-containing acylating agent

The P₀ glycoprotein of peripheral nerve myelin