Plasma extravasation and neuropeptide release in human skin as measured by intradermal microdialysis

doi:10.1016/S0304-3940(97)00494-1

Neuroscience Letters

Volume 230, Issue 2, 18 July 1997, Pages 117-120

https://doi.org/10.1016/S0304-3940(97)00494-1 Get rights and content

Abstract

Time course of plasma extravasation in neurogenic inflammation elicited by histamine iontophoresis and topical capsaicin application was measured in vivo by a modified intradermal microdialysis technique in humans. Single linear plasmapheresis fibers were inserted intracutaneously at the volar forearm and perfused with Ringer's solution (4 μl/min) with one microdialysis fiber located at the planned stimulation site and a second inside the axon reflex area. Protein concentration in the dialysate increased by 300% in the histamine wheal but no increase was found in the surrounding flare area. There was no protein extravasation in the skin treated with capsaicin nor in the surrounding flare region. Inside the histamine-induced flare reaction a significant increase of calcitonin gene related peptide (CGRP) was found, whereas the increase of substance P (SP) was not significant.

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Acknowledgements

This work was supported by the Deutsche Forschungsgemeinschaft (SFB 353). We would like to acknowledge Professor Handwerker for his fruitful comments on the manuscript.

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Plasma extravasation and neuropeptide release in human skin as measured by intradermal microdialysis

Abstract

Section snippets

Acknowledgements

J. Invest. Dermatol.

Anal. Biochem.

J. Invest. Dermatol.

J. Invest. Dermatol.

J. Allergy Clin. Immunol.

J. Pharmacol. Toxicol. Methods

Eur. J. Pharmacol.

The nonpeptide Nk1 receptor antagonist Sr140333 produces long lasting inhibition of neurogenic inflammation, but does not influence acute chemonociception or thermonociception in rats

Naunyn-Schmiedeberg's Arch. Pharmacol.

Ethanol absorption across human skin measured by in vivo microdialysis technique

Acta Derm. Venereol. Stockh.

Histamine is released from skin by substance P but does not act as the final vasodilator in the axon reflex

Br. J. Pharmacol.

A quantitative comparison of the effect of local analgesics on argon laser induced cutaneous pain and on histamine induced wheal, flare and itch

Acta Derm. Venereol.