Abstract
Neutrophils utilize Fcγ Receptors (FcγR) to bind and internalize antibody coated cells or immune complexes. We have compared ultrastructurally FcγR-mediated phagocytosis by neutrophil cytoplasts (enucleated and granule-free neutrophils) prepared either by treatment with cytochalasin B (CB-cytoplasts) followed by ultracentrifugation or by brief heating at 45°C in suspension followed by ultracentrifugation. The phagocytosis of antibody-coated erythrocytes (EIgG) or the internalization of crosslinked IgG complexes by cytoplasts prepared by brief heating was comparable to that of untreated neutrophils. In contrast, CB-cytoplasts bound but failed to internalize EIgG or crosslinked IgG complexes. Comparison of these two methods for the preparation of neutrophil cytoplasts may assist in clarifying the signal transduction requirements involved following ligand binding to FcγR which initiate phagocytosis.
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Fleit, H.B., Lane, B.P. Fcγ Receptor Mediated Phagocytosis by Human Neutrophil Cytoplasts. Inflammation 23, 253–262 (1999). https://doi.org/10.1023/A:1020226020062
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DOI: https://doi.org/10.1023/A:1020226020062