Abstract
TIRF microscopy can be used in conjunction with CFP/YFP FRET to detect movements of the cytoplasmic tails of GIRK channels (Riven et al., this issue of Neuron). This innovative combination of techniques allows molecular resolution of small motions underlying ion channel activation by G proteins and will likely find widespread use for study of membrane-associated molecules.
MeSH terms
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Bacterial Proteins / metabolism*
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Cell Membrane / metabolism*
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Cell Membrane / ultrastructure
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Fluorescence Resonance Energy Transfer*
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Fluorescent Dyes
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Green Fluorescent Proteins
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Luminescent Proteins / metabolism*
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Microscopy, Fluorescence / instrumentation
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Microscopy, Fluorescence / methods*
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Potassium Channels / metabolism
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Signal Transduction / physiology
Substances
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Bacterial Proteins
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Fluorescent Dyes
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Luminescent Proteins
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Potassium Channels
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yellow fluorescent protein, Bacteria
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Green Fluorescent Proteins