Study the normal anatomical and variation of the recurrent artery of Heubner (RAH) in fifty Thai cadaveric brains were studied under operating microscope. The anterior communicating artery (ACoA) located at the orbital surface of gyrus rectus about 50% and medial surface of gyrus rectus about 50%. The recurrent artery of Heubner originated from the anterior cerebral artery part 2 (ACA2) 60.4%, junction of ACoA 31.9%, and anterior cerebral artery part 1 (ACA1) 7.7%. Ninety-two percent of the origins of the RAH were identified within 2 mm from the proximal and distal of ACoA. The most common site of its origin from main artery was at its lateral surface 91.0%. The courses of RAH in relation to ACA1 segment were found anteriorly 63.4%, superiorly 29.9% and posteriorly 6.7%, respectively.