The influence of folic acid on the picrotoxin-sensitive site of the GABAa-receptor complex

C M Van Rijn; T J Van der Velden; J F Rodrigues de Miranda; M G Feenstra; O R Hommes

doi:10.1016/0920-1211(88)90058-7

The influence of folic acid on the picrotoxin-sensitive site of the GABAa-receptor complex

Epilepsy Res. 1988 May-Jun;2(3):215-8. doi: 10.1016/0920-1211(88)90058-7.

Authors

C M Van Rijn¹, T J Van der Velden, J F Rodrigues de Miranda, M G Feenstra, O R Hommes

Affiliation

¹ Dept. of Experimental Neurology, University of Nijmegen, The Netherlands.

PMID: 2848698
DOI: 10.1016/0920-1211(88)90058-7

Abstract

The site of action responsible for the convulsive effect of folic acid was investigated in vitro. Folic acid (ECmax 5 x 10(-4) M) enhances the binding of the cage convulsant [3H]t-butylbicycloorthobenzoate ([3H]TBOB) to rat brain membranes, namely to 130% of control in the absence of GABA and to over 300% of control in the presence of physiological concentrations of GABA. Analysis of the binding parameters reveals that folic acid increases the apparent number of [3H]TBOB binding sites.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Binding, Competitive
Bridged Bicyclo Compounds / metabolism
Bridged Bicyclo Compounds, Heterocyclic*
Convulsants / metabolism*
Folic Acid / metabolism*
Male
Picrotoxin / metabolism*
Rats
Rats, Inbred Strains
Receptors, GABA-A / drug effects
Receptors, GABA-A / metabolism*

Substances

Bridged Bicyclo Compounds
Bridged Bicyclo Compounds, Heterocyclic
Convulsants
Receptors, GABA-A
Picrotoxin
Folic Acid
tert-butylbicyclo-2-benzoate