Abstract
Reverse transcriptase-polymerase chain reaction (RT-PCR) on 24 cerebrospinal fluid (CSF) specimens collected between February and August 1992 detected genome sequence of West Nile (WN) virus in 8 specimens and Japanese encephalitis (JE) virus in a single specimen. The results, combined with the data by IgM-ELISA on CSF indicated that a significant proportion of acute encephalitis cases in Karachi, Pakistan, were caused by WN virus infection, while JE virus caused a small fraction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acute Disease
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Base Sequence
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Cerebrospinal Fluid / virology
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Child
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Child, Preschool
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DNA Primers / chemistry
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DNA, Viral / analysis
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Encephalitis Virus, Japanese / genetics*
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Encephalitis Virus, Japanese / isolation & purification
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Encephalitis, Japanese / cerebrospinal fluid*
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Encephalitis, Japanese / virology*
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Female
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Genome, Viral*
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Humans
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Infant
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Male
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Molecular Sequence Data
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West Nile Fever / cerebrospinal fluid*
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West Nile Fever / virology*
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West Nile virus / genetics*
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West Nile virus / isolation & purification