Abstract
There is evidence that leukocytes play an important role in mediating tissue injury during acute ischemic stroke. Endothelial cell adhesive molecules such as ICAM-1 are required for the migration of leukocytes into the brain. Using an Elisa, we compared the expression of ICAM-1 by human brain microvascular endothelial cells with human umbilical vein endothelial cells. There was constitutive surface expression of ICAM-1 on both brain and umbilical vein endothelial cells. With cytokine (IL-1 beta or TNF) or lipopolysaccaride stimulation, ICAM-1 surface expression increased to a greater extent on brain than on umbilical vein endothelial cells. Dexamethasone at doses up to 100 microM had no effect on inhibiting cytokine-mediated upregulation of ICAM-1 on human brain microvascular endothelial cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antigens, CD / biosynthesis
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Blotting, Northern
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Cell Adhesion Molecules / analysis
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Cell Adhesion Molecules / biosynthesis*
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Cerebrovascular Circulation*
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Cerebrovascular Disorders / physiopathology
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Endothelium, Vascular / cytology
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Endothelium, Vascular / drug effects
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Endothelium, Vascular / metabolism*
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Enzyme-Linked Immunosorbent Assay
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Gene Expression / drug effects
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Humans
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Intercellular Adhesion Molecule-1
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Interleukin-1 / pharmacology
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Lipopolysaccharides / pharmacology
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Microcirculation*
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RNA, Messenger / analysis
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Transcription, Genetic / drug effects
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Tumor Necrosis Factor-alpha / pharmacology
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Umbilical Veins
Substances
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Antigens, CD
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Cell Adhesion Molecules
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Interleukin-1
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Lipopolysaccharides
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RNA, Messenger
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Tumor Necrosis Factor-alpha
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Intercellular Adhesion Molecule-1