Three cell lines, VM/Dk P497 P540 and P560 derived from the VM spontaneous murine astrocytoma have previously been fully characterised and found to differ in their degree of astrocytic differentiation. The in vitro chemosensitivity of the three lines has been investigated using the 35S-methionine uptake assay. Differential chemosensitivity was found to exist between the cell lines. The pattern of chemosensitivity in relation to astrocytic differentiation was complex but the least differentiated cell line, P497, tended to be the least chemosensitive.
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