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Volumetric magnetic resonance imaging study of the brain in subjects with sex chromosome aneuploidies
  1. Michael M Warwicka,
  2. Gillian A Doodya,
  3. Stephen M Lawriea,
  4. Julia N Kestelmana,
  5. Jonathan J K Besta,b,
  6. Eve C Johnstonea
  1. aDepartment of Psychiatry, Royal Edinburgh Hospital, Edinburgh, UK, bMagnetic Resonance Imaging Unit, City Hospital, Edinburgh, UK
  1. Dr MM Warwick, Department of Psychiatry, Royal Edinburgh Hospital, Edinburgh, EH10 5HF, UK.


OBJECTIVES Cognitive impairment has been reported in people with sex chromosome aneuploides (SCAs) and it has been proposed that the presence of an extra sex chromosome may have an adverse effect on neurodevelopment. This study examines the hypothesis with structural MRI of the brain.

METHODS Thirty two subjects with SCA (XXX (n=12), XYY (n=10), and XXY (n=10)) from a birth cohort study were matched groupwise for age, parental social class, and height with normal controls (13 female, 26 male). Brain MRI , measurements of IQ, and a structured psychiatric interview were performed.

RESULTS The XXX females and XXY males had significantly smaller whole brain volumes than controls of the same phenotypic sex (p=0.003 and p⩽0.05 respectively). The XXY group also had bilaterally enlarged lateral ventricles (p⩽0.05). No significant differences were found between the XYY group and controls. IQ scores in all SCA groups were lower than in the control groups.

CONCLUSIONS The main result of reduced brain volumes in XXX and XXY subjects, but not in XYY subjects, indicates that the presence of a supernumerary X chromosome has a demonstrable effect on brain development.

  • sex chromosome aneuploides
  • magnetic resonance imaging
  • schizotypy

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Over the past 50 years refinement in genetic techniques has enabled the identification and subsequent characterisation of sex chromosome aneuploidies (SCAs). Early studies focused on the phenotypic manifestations of the XYY genotype, in both institutional and general populations.1-4 After the report of Witkinet al 5 of a link between criminality and IQ in XYY males, others have examined the association between supernumerary X chromosomes and IQ.

Several prospective cohort studies, begun in the 1970s, of people with SCAs identified at birth reported preliminary findings in the early 1980s.6 7 The findings suggested that there was impaired performance in both verbal and non-verbal IQ in girls with the XXX genotype and delayed language development in XXY boys. It was hypothesised that there is a continuum of language disability in boys with Klinefelter’s syndrome (XXY)6 and the idea that an extra sex chromosome may have an adverse effect on neurodevelopment was proposed.8

People with Turner’s syndrome (XO) show evidence of impaired modular spatial cognition9 10 and structural neuroimaging studies of SCAs have focused on this condition.11 12 Bilateral structural abnormalities of the hippocampi, caudate, lenticular, and thalamic nuclei and parieto-occipital matter, more marked on the right side, have been shown relative to controls. Two functional imaging studies of Turner’s syndrome have been performed13 14and global hypermetabolism with relative bilateral hypometabolism of the association cortices reported. The inclusion of mosaic subjects in the latter study has lent biological support to the “gene dosing hypothesis” proposed by Murphy et al.14

There have, to our knowledge, been no other group structural or functional neuroimaging studies of people with XXX, XYY, or XXY to date. This study examines the hypothesis that the presence of an extra sex chromosome has an effect on neurodevelopment using structural MRI of the brain in people with XXX, XYY, and XXY genotypes.


Forty subjects with SCA who participated in the Edinburgh study of growth and development of children were retraced and asked to participate in the current study. The full methodology of this study of consecutive liveborn infants has already been reported.15The study cohort are now aged between 16 and 28 years. Thirty two of the subjects with SCA consented to this study (47 XXX (n=12), 47 XYY (n=10), and 47 XXY (n=10)) and none of these were known to have any concomitant physical disorder affecting brain function. The subjects with SCA were matched groupwise to normal controls (13 female, 26 male, also from the cohort study) of the same phenotypic sex for age, parental social class, and height (within 5 cm).

Recent work has shown that none of these subjects fulfil criteria for major psychiatric disorder, although there was an indication that they have an excess of schizoid/schizotypal personality traits.16 17 The structured interview for schizoptypy (SIS)18 was therefore used to measure schizotypal personality traits for this investigation. Global SIS scores were scaled 1–7, with a score of 1 as maximal. The SIS, national adult reading test (NART)19 of premorbid IQ, and the Quick test20 of current IQ were administered to subjects by one investigator (MMW), who was blind to their genotypic status.

Subjects underwent MRI on a 1 tesla Siemens (Erlangen, Germany) Magnetom scanner. Midline sagittal localisation was followed by two sequences to image the whole brain. The first scan was a three dimensional turboFLASH (MPRAGE) sequence consisting of a 180° inversion pulse followed by a FLASH collection (flip angle 12°, TR=10 ms, TE=4 ms, TI=200 ms, and relaxation delay time 500 ms). This scan gave 128 contiguous slices, each 1.88 mm thick. The images were taken in the coronal plane orthogonal to the Talairach plane. The field of view was 250 mm. The second scan was a double spin echo sequence which gave simultaneous proton density and T2 weighted images (TR=3565 ms, TE=20 and 90 ms). Thirty one contiguous 5 mm slices were acquired in the Talairach plane with a field of view of 250 mm.

Immediately after each subject’s scan a large plain test object filled with light oil was scanned in exactly the same place in the coil and in the same orientation as the subject. The test object data was used to correct for inhomogeneity in the radiofrequency coil (because of this the signal fell off at the extremes of the volume scanned in the vertical axis). The mean intensity of a 5×5 pixel square in the centre slice of the test object data was taken as the optimum intensity. The whole dataset was normalised to this value. By dividing the patient data by the normalised data any deviation from optimum coil response could be corrected.

Image processing was done on Sun Microsystems workstations using the software package “Analyze” (Mayo Foundation, Rochester, MN, USA) to outline and compute neuroanatomical structures. Structures were identified with the assistance of an MRI atlas and volumes were calculated by summing voxels on all brain slices included. Specific regions of interest selected initially were whole brain, right and left (R+L) pre-frontal lobes, temporal lobes (R+L), caudate nuclei (R+L), lentiform nuclei (R+L), thalamic nuclei (R+L), amygdalohippocampal complex (R+L), lateral ventricles (R+L), third ventricle, and fourth ventricle. The prefrontal, temporal, and amygdalohippocampal volumes were defined according to standard criteria and the remaining regions of interest were outlined by naturalistic boundaries, as in our previous studies.21 Asymmetry between groups was assessed for volumes of the lateral ventricles, prefrontal lobes, temporal lobes, and amygdalohippocampal complexes using the formula R-L/R+L.

All image processing was done, blindly, by one investigator (JNK) who compared her results with those of another rater on all brain regions in five brains (Pearson’s r=0.8–1.0). The scans were identified by code number/date of birth only and the investigator performing the analyses was unaware of the status of the subjects.

The second SE scan was examined by a neuroradiologist (JJKB) for any qualitative morphological abnormalities—to exclude gross lesions and to quantify the numbers of high intensity signal (HIS) foci. HIS foci were recorded, if at least 2 mm in size, by location.

Statistical analysis of results was conducted using SPSS (Microsoft), with separate analyses for the phenotypic females and males. Analysis of variance (ANOVA) with post hoc Scheffe tests (controlling for multiple comparisons) if required were applied to examine group differences in age, height, and IQ. Mann-WhitneyU and χ2 tests were used to examine group differences in parental social class, handedness and SIS scores. Group differences in MRI volumes were examined with an ANOVA and post hoc Scheffe tests, controlling for whole brain volume by taking the proportion of each regional volume to whole brain volume. There is, however, some evidence that this approach may not reliably control for whole brain volumes and the volumes of brain regions may also be influenced by body size. We therefore performed analyses of covariance for significantly abnormal brain regions, controlling for whole brain volume and height (as a proxy for body size). Pearson’s correlation coefficients were computed to explore the relation between whole brain volumes and IQ as measured by full scale NART and the Quick; Spearman’s correlation coefficients to examine associations between SIS scores that differed between the study groups, whole brain volumes, and IQ.



As shown in tables 1 (females) and 2 (males), patients with SCA and controls of the same phenotypic sex were well matched for age, parental social class, and handedness. Despite matching for height, the XYY group (mean height 188 cm) were significantly (p<0.05) taller than the XY subjects (mean height 183 cm). As would be expected, the IQ scores in all three SCA groups were lower on both the NART and the Quick test than their same phenotypic sex controls, but there were no significant IQ differences between the two male SCA groups (XYY and XXY).

Table 1

Demographics: phenotypic females

Table 2

Demographics: phenotypic males

Subjects’ SIS scores were dichotomised into those scoring 1, 2, or 3 (high scores) and those subjects scoring 4, 5, 6, or 7 (low scores). XXX females were overrepresented, relative to female controls (XX), in the high scoring groups on introversion (Fisher’s exact two tailed p=0.01), magical thinking (Fisher’s p=0.04), and impulsivity (Fisher’s p=0.002). Subjects with XYY were overrepresented in the antisocial traits higher score group compared with normal male controls (Fisher’s p=0.03). There were no significant differences in dichotomised SIS scores between subjects with XXY and normal male controls (XY).


One XXX and one male control were not scanned. As shown in table3, XXX females had significantly smaller whole brain volumes than female controls. Several other substantial differences in regional volumes are evident, but were not significant after controlling for whole brain volume. To control for the possibility that whole brain volume reductions in XXX subjects were attributable to general reductions in body size, even though mean height was slightly greater in subjects with XXX, we performed an analysis of covariance of whole brain volume by group and height. This confirmed a significant effect for group (p=0.002) but not for height (p=0.2). There were no significant volumetric asymmetries detected between phenotypic females.

Table 3

MRI Results: phenotypic females. Group mean (SD) regional brain volumes in ml are given for information. Proportions of regional to whole brain volume were used for statistical analysis

Within the phenotypic males, no significant differences were found between XYY and controls, but several volume differences were found between XXY and controls (table 4). The XXY males had smaller brains than controls, as well as bilaterally enlarged lateral ventricles relative to whole brain volume. The right lateral ventricular volume was also larger in XXY subjects than XYY subjects. Covariance analysis of whole brain findings with group and height confirmed a significant effect for group (p=0.001) but not for height (p=0.5). Similarly, the ventricular findings in XXY subjects remained highly significant after covariance analysis on whole brain volume and height. There were no significant volumetric asymmetries detected between phenotypic males.

Table 4

MRI results: phenotypic males. Group mean (SD) regional brain volumes in ml are given for information. Proportions of regional to whole brain volume were used for statistical analysis

One or more HIS foci were detected in three XXX females, but no female controls (Fisher’s exact test p=0.08). One or more HIS foci were also detected in five XXY subjects (p=0.001) and one XYY subject (p=0.3) whereas none were seen in any male controls.


Within all female subjects, whole brain volumes were correlated with the full scale NART (r=0.58, p=0.004) and Quick test (r=0.53, p=0.008) scores. In men, weaker correlations were found with the NART (r=0.30, p=0.05) and the Quick test (r=0.31, p=0.04). Whole brain and total lateral ventricular volume correlated with Quick IQ scores when all subjects with SCA were analysed as one group (r=0.38, p=0.04;r=0.62, p<0.001), This was not replicated in normal controls. There were no significant correlations between IQ scores (either NART or Quick) and whole brain volumes in any individual group (either SCA subjects or normal controls).

None of the three significant different SIS scores in the XXX group (introversion, magical thinking, and impulsivity) correlated with whole brain volume. However, in the normal female control group, impulsivity did show a negative correlation with whole brain volume (r=−0.57, p=0.04).

In male subjects, antisocial traits were correlated negatively with whole brain volume in the normal control group (r=−0.52, p=0.01), but not the XYY (r=0.12, p=0.7), or XXY groups (r=−0.44, p=0.2).


We have found differences in the brain volumes of subjects with SCAs as compared with healthy controls of the same phenotypic sex. The previous careful collection of these consecutively born cases gave us the opportunity to study this unusual population with modern brain imaging and analysis techniques of high resolution. Our main result, of significantly reduced brain volumes in subjects with XXX and XXY, but not in XYY subjects, indicates that the presence of a supernumerary X chromosome has a demonstrable effect on brain development.

The number of subjects with SCAs is small, but it is one of the largest known cohorts of such subjects. Moreover, the effects we detected are substantial and are not affected by subject ascertainment bias. We do not have a measure of total intracranial volume as a control reference for whole brain volumes; it is therefore possible that the brain volume reductions are attributable to general reductions in skull or body size. However, there is no one correct method of controlling for these possible influences22-24 and covariance analysis for height is generally regarded as a satisfactory way of controlling for body size.23 Moreover, the fact that subjects with SCAs were generally taller than their same sex controls supports our conclusion that the brain itself is significantly smaller in subjects with SCAs than healthy controls. It should also be noted that subjects with XXY had enlarged lateral ventricles whether controlling for whole brain volume as a proportion or by covariance.

The correlations between whole brain volume and IQ seen in both the SCA cohort and phenotypic females, suggest that an association between brain size and IQ does exist in these two groups. Others have reported similar findings in normal subjects, especially females25and patients with schizophrenia.21 Presumably, the developmentally small brains in subjects with SCAs influence their “premorbid” and current intelligence level, as measured by the NART and Quick tests. There are, of course, several environmental influences on brain size and IQ, such as social class and lifestyle factors. Our cases were matched for paternal social class and this is, therefore, not a probable explanation of our findings.

Our findings of smaller brains and more HIS foci in subjects with SCAs also have more general neurodevelopmental relevance. Recent imaging studies have shown that X linked conditions (for example, fragile X syndrome, X linked pyruvate dehydrogenase deficiency) influence brain development.26 27 Furthermore, X linked subcortical laminar heterotopia and lissencephaly syndrome is a neuronal migration disorder manifested by abnormal configuration of the six layers of the neocortex. It has recently been linked to mutations of a novel CNS gene which is highly expressed during brain development in fetal neurons.28 The importance of the X chromosome in ensuring normal fetal neurodevelopment is now well established. Indeed, the total absence of an X chromosome, as in Turner’s syndrome, is also associated with brain abnormalities.11 12 We have shown that the presence of a supernumerary X chromosome also seems to adversely affect brain development. The finding that the presence of an extra Y chromosome was not associated with significant volumetric brain differences relative to normal male controls may partly reflect low power, but any such effect is at the very least not as dramatic as an extra X. The excess of HIS foci in subjects with a supernumerary X chromosome is of uncertain pathological relevance. Generally HIS foci are thought to reflect the effects of age and any head trauma.29 Their presence in XXX and XXY subjects suggests that a developmentally abnormal brain may be more sensitive to such possible insults, or that subjects with SCA s may have an excess of unreported minor head injuries relative to the normal controls.

The correlations between the SIS and brain volumes also suggest that reduced brain volumes may have a role in predisposing to certain behaviours. Some of these could even be mediated by low IQ. However, these analyses were exploratory, could have generated chance findings, and cannot specify a particular causal pathway.

These findings resemble those found in schizophrenia and other neuropsychiatric disorders of presumed developmental origin.30 Quantitative reviews of the literature have reported that patients with schizophrenia have substantially reduced whole brain volumes and enlarged lateral ventricles compared with healthy controls.31 32 Further, there seems to be an excess of XXX and XXY genotypes in people with schizophrenia.33 Longitudinal studies of children with supernumerary X chromosomes have found language deficits, with relative preservation of spatial skills, to be present in some cases. Studies of neurodevelopment in schizophrenia have identified language and motor deficits in childhood before the onset of psychosis in some cases.34-36 It has also been reported that people with a diagnosis of mild learning disability have a higher point prevalence (about 3%) of schizophrenia than the normal population.37

Finally, as described above, at the time of scanning none of the subjects in this study fulfilled diagnostic criteria for major mental illness, but they are just entering the period of risk for such disorders. The subjects with SCAs in this study do have more evidence of behavioural disturbance and features of asociality16 17 and one subject, who will be the subject of a separate report, developed a psychosis subsequent to being scanned. It remains to be seen whether more subjects will develop psychosis and whether this may be related to SIS or MRI findings.


MMW was the principal investigator and wrote the first draft of the paper. GAD and SML conducted the data analysis. JNK conducted the quantitative MRI analysis, supervised by SML. JJKB devised the imaging protocols and conducted the qualitative MRI analysis. ECJ designed the study and supervised it throughout. All the authors commented on drafts of the paper. This work was supported by the Wellcome Trust; MMW was a Wellcome research registrar. We are grateful to Dr Shirley Ratcliffe for giving us access to this most interesting cohort and enriching us with her knowledge of the subject and the individuals, to Annette Blane and Jill Rollo for conducting the MRI scans, to Dr Ewen Rimmington for technical assistance in developing MRI techniques, to Dr Robert Sandler for assistance in matching subjects, to Dr Anne Hodges for assistance with the administration of the SIS and to Miranda Maxwell and Catherine McLeod for supervising the subjects in the MRI scanner. Finally, we thank all the people who took part in the study.


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