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Intramedullary tuberculoma mimicking primary CNS lymphoma
  1. A A Mohit1,
  2. P Santiago2,
  3. R Rostomily1
  1. 1Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA, USA
  2. 2Department of Neurological Surgery, Washington University School of Medicine, St Louis, MO, USA
  1. Correspondence to:
 A A Mohit
 Department of Neurological Surgery, University of Washington School of Medicine, Seattle, WA, USA;


The incidence of primary central nervous system lymphoma (PCNSL) has been on the rise in the setting of immunodeficiency syndromes such as acquired immune deficiency syndrome (AIDS). Its diagnosis has been facilitated by the advent of a cerebrospinal fluid (CSF) Epstein–Barr virus (EBV) PCR assay. The reported high sensitivity and specificity of this assay has made it the cornerstone of diagnosis of PCNSL, replacing more traditional methods such as an open CNS biopsy. Here, we have described a patient with a known history of C3 AIDS presenting with lower extremity weakness and eventual myelopathy who was later diagnosed as having intramedullary PCNSL after detection of EBV DNA in his CSF. After failing to respond to radiotherapy, he underwent a spinal cord biopsy revealing intramedullary tuberculoma. This case illustrates the risk of misdiagnosis with this assay and the importance of histological confirmation of a pathological lesion prior to implementation of therapy.

  • AFB, acid fast bacilli
  • AIDS, acquired immune deficiency syndrome
  • EBV, Epstein–Barr virus
  • MRI, magnetic resonance imaging
  • PCNSL, primary central nervous system lymphoma
  • PCR, polymerase chain reaction
  • AIDS
  • spinal cord
  • tuberculoma

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There has been a dramatic increase in the incidence of primary central nervous system lymphoma (PCNSL) in the setting of acquired immune deficiency syndrome (AIDS).1 This entity is defined as lymphoma limited to the brain and spinal cord without systemic disease. As with other AIDS related lymphomas, these are also aggressive B cell neoplasms, either diffuse large cell or diffuse immunoblastic non-Hodgkin’s lymphoma. However, unlike AIDS related systemic lymphomas where only 30–50% of tumors are associated with Epstein–Barr virus (EBV) infection, AIDS related PCNSL have a 100% association with EBV.2

Accurate and timely diagnosis of AIDS related PCNSL has been challenging since its clinical presentation resembles that of toxoplasmosis and other opportunistic infections.3 The definitive diagnosis of this condition can only be made with a CNS biopsy.4 However, this poses many risks to the patients and surgical teams. As a result, it has become a common practice in many medical centres to presumptively diagnose patients with PCNSL if they fail to respond to antitoxoplasmosis regimens.5 More recently, the detection of EBV DNA in cerebrospinal fluid (CSF) samples of nearly all patients with PCNSL has established this assay as a diagnostic test for this condition.6–9

Here we present a case of biopsy proven intramedullary tuberculoma in a patient with AIDS previously diagnosed as having intramedullary PCNSL after EBV DNA was detected in his CSF. He was presumptively diagnosed with PCNSL and underwent a course of radiation therapy. After a lack of response, he underwent an open biopsy of this cord lesion for definitive diagnosis.


This 50 year old man initially presented with inguinal lymphadenopathy and back and left sided leg pain in July 2001. Approximately one month later he developed lower extremity weakness which manifested in a fall down the stairs. He had no evidence of bowel or bladder dysfunction at that time. A magnetic resonance imaging (MRI) scan of his entire spine revealed a T2 bright intramedullary lesion at T9/T10 levels with cord expansion. The lesion brightly enhanced after administration of contrast (fig 1A, B). A work-up revealed positive HIV serology with a viral load of 750 000 copies/ml and a CD4 count of 43/μl. He was started on a regimen of stavudine (d4T), lamivudine (3TC), and efavirenz, for management of AIDS as well as pyrimethamine and sulfadiazine for a presumptive diagnosis of intramedullary toxoplasmosis.

Figure 1

 Contrast-enhanced (CE) MRI demonstrating the intramedullary lesion at T9. Note minimal cord expansion and the lack of meningeal enhancement. (A) Preoperative sagittal CE T1 images demonstrating the lesion in the substance of the spinal cord (arrow). (B) Preoperative axial CE T1 images demonstrating the involvement of the central portion of the cord. (C) Postoperative sagittal CE T1 images demonstrating the proper biopsy sampling of the lesion.

After a two week period, the patient began to have episodic urinary incontinence with further progression of lower extremity weakness to the point of inability to ambulate. A lumbar puncture at that time showed slightly increased protein (67 mg/dl; reference range 15–45 mg/dl), normal glucose and 22 cells/μl (98% lymphocytes). Polymerase chain reaction (PCR) analysis of CSF showed presence of EBV DNA with a viral titre of 660 copies/ml. The patient was diagnosed as having primary CNS lymphoma and was started on a regimen of radiation therapy. He underwent seven sessions of 180 cGy of external beam therapy with two additional boosts of 2200 cGy. His lower extremity strength continued to deteriorate and he was unable to stand a few weeks after conclusion of radiation therapy. Repeat imaging showed the persistence of the intramedullary lesion. Surveillance MRI of the entire neuraxis did not reveal additional lesions at this time.

The patient underwent an open biopsy of this lesion in the thoracic spinal cord. Postoperative MRI confirmed proper sampling of the lesion (fig 1C). Histological analysis showed non-caseating granulomas surrounded by reactive gliosis and increased scattered histiocytes. Acid fast bacilli (AFB) stain demonstrated presence of numerous AFB in multiple specimens (fig 2). Gomori methenamine silver (GMS) stain for fungi and Giemsa stain for toxoplasma were negative. There was no evidence of lymphoproliferation. The patient was started on a four drug antituberculosis (TB) regimen. Further enquiry of his past medical history revealed a history of exposure to tuberculosis in the 1950s as a child, at which time his father and brother were hospitalised in a TB sanitarium.

Figure 2

 Histological demonstration of the intramedullary tuberculoma and acid fast bacilli. (A) H&E section of the biopsied specimen shows a granuloma with epithelioid histiocytes (arrows) (×40). (B) Acid-fast bacilli (arrows) in the biopsied specimen (×100).

Approximately three months after transfer to a skilled nursing facility, he presented with increased confusion and headaches, along with nausea and vomiting. An MRI of the entire neuraxis revealed new intraparenchymal lesions in the pons and cervicomedullary junction which were hypodense on T1 and hyperdense on T2 sequences. In addition, increased T2 signal was identified diffusely in the subependymal region throughout the cerebral hemispheres. CSF studies revealed 360 WBC/HPF with 80% lymphocytes, protein 2072 mg/dl, and low glucose. As these findings were suggestive of disseminated TB cerebritis/meningitis he was continued on anti-TB therapy. He continued to deteriorate neurologically and died two weeks later of complications from a bout of pneumonia.


Prior to the advent of CSF-EBV PCR assay, the difficulty in diagnosis of intrinsic spinal cord lesions in the setting of AIDS had led to the adoption of a practical approach to the management of these patients. This approach included treating a CNS mass lesion in an AIDS patient as though it were toxoplasmosis first, and then if the patient did not respond to antitoxoplasmosis regimens, as a lymphoma, which often included radiation treatment.10,11 A biopsy of the lesion can eliminate some of the uncertainty associated with this approach. However, CNS biopsy is invasive and in 5–33% of cases no diagnosis can be reached due to sampling problems or tissue non-viability.12,13

Recently, detection of CSF-EBV DNA by PCR in AIDS patients has been reliably associated with primary CNS lymphoma. The basis for this approach is the observation that nearly all AIDS associated primary CNS lymphomas occur in the setting of EBV infection.2 EBV DNA has been detected in the CNS of nearly all patients with CNS lymphoma and almost never (1/86 patients) in the CNS of patients without it. Multiple studies have reported the utility of a PCR assay for detection of EBV DNA in the CSF of AIDS patients with PCNSL.6,7,9,14 The specificity and sensitivity reported from these studies are generally 90–100% with positive and negative predictive values reaching near 100%. Cingolani et al reported that a positive PCR was not only predictive of CNS involvement, but it also preceded identification of lymphomatous lesions on MRI by a mean of 35 days.9 al-Shahi et al have reported detection of EBV DNA in CSF samples as early as 17 months prior to imaging abnormalities.8 These observations have led to the acceptance of this assay as an important tool for diagnosis of PCNSL.

Although PCNSL and toxoplasmosis are the most common space occupying lesions in the CNS in patients with AIDS, TB which can also present with mass effect should be included in this differential diagnosis. The incidence of pulmonary TB is almost 500 times greater in AIDS patients compared with the general population.15 Furthermore, the incidence of CNS TB is dramatically increased in AIDS patients.16 Although meningitis is the most common presentation of TB in the CNS, it can take a variety of forms including cerebritis, abscess, or tuberculoma.17 Intramedullary tuberculomas, although rare, usually present with myelopathy which can be rapidly progressive, much like the symptoms described in this case.18–20

Clinical symptomatology and presentation of intramedullary tuberculomas can be virtually indistinguishable from PCNSL and toxoplasmosis.21 These patients present with a subacute paraparesis that progresses over one or two months.22 In most patients evaluation of CSF reveals an active inflammatory response with pleocytosis, hypoglycorrhachia and a very high protein level.15,23 The MRI features of CNS tuberculomas include an isointense appearance of the grey matter on T1-weighted images and may have a slightly hyperintense rim. On T2-weighted images the appearance can vary depending on the pathological state of the tuberculoma, ranging from isointense to hypointense.24,25

TB, PCNSL, and toxoplasmosis can have very similar clinical and radiological characteristics especially in the setting of AIDS where atypical features of the pathologies may be present. The advantages of a non-invasive method for differentiating among these are clear. The CSF-EBV PCR assay is highly sensitive and specific for AIDS related PCNSL and has quickly become an alternative to biopsy and histological diagnosis. Although this approach may circumvent risks associated with biopsy and can result in early therapy, we believe that the AIDS clinician should be aware of the risk of misdiagnosis, as demonstrated by this case.



  • Competing interests: none declared