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WED 183 Cladribine tablets effects on t cell subsets in patients with early ms
  1. Olaf Stuve1,
  2. Per Soelberg-Sorensen2,
  3. Thomas Leist3,
  4. Yann Hyvert4,
  5. Doris Damian4,
  6. Ursula Boschert4
  1. 1University of Texas, Southwestern Medical Center, Dallas, TX, USA
  2. 2Department of Neurology, University of Copenhagen, Rigshospitalet, Copenhagen, Denmark
  3. 3Jefferson University, Comprehensive MS Center, PA, USA
  4. 4EMD Serono Research and Development Institute, Inc., a business of Merck KGaA, Darmstadt Germany


Background Because of the durable clinical effects of cladribine tablets 3.5 mg/kg (CT3.5) in patients with multiple sclerosis, the influence of treatment on cells with regulatory immune function is of interest.

Objective Examine effects on central memory; effector memory; and naturally occurring regulatory (nTregs) CD4 +T cells after first administration of CT3.5 in ORACLE-MS.

Methods Peripheral blood T-lymphocytes were immunophenotyped at baseline, and Weeks 5, 13, 24, 48 in CT3.5 treated patients (n=41). Absolute numbers and proportions of central memory (CD4+RO+CCR7+), effector memory (CD4+RO+CCR7-), Th1-type (CD4+CXCR3+), nTregs (CD4+CD25+CD127-), including naïve-like and memory-like nTregs were measured.

Results Greatest median reductions in absolute numbers occurred at Week-13 for effector memory cells (−54%); Week-24 for central memory (−63%) and Th1-type cells (−51%); with similar/slightly increased levels at Week-48. There was ~5% reduction in proportion of central memory cells, but no change in proportions of effector memory and Th1-type cells. Absolute numbers of nTregs (−48%), naïve-like (−67%) and memory-like nTregs (−42%) decreased by Week-48. nTregs and naïve-like nTregs proportions were unchanged. Proportions of memory-like nTregs slightly increased up to 48 Weeks.

Conclusion CT3.5 administration has a comparable effect on CD4+ T cell subpopulations, with no dramatic shifts in proportions.


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