Article Text

Download PDFPDF
A11 Skin fibroblasts from huntington´s disease patients show distinct signature of MIRNAS expression along disease progression
  1. Veronica Brito1,2,3,
  2. Ruben Fernandez-Santiago3,4,
  3. Mario Ezquerra3,4,
  4. Esther Sieiro1,2,3,
  5. Jesus Perez-Perez3,4,5,6,7,8,
  6. Jaime Kulisevsky5,6,9,
  7. Silvia Gines1,2,3
  1. 1Departament de Biomedicina, Facultat de Medicina, Institut de Neurosciències, Universitat de Barcelona, Barcelona, Spain
  2. 2Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain
  3. 3Centro de Investigación Biomédica, Barcelona, Spain
  4. 4Laboratory of Parkinson Disease and Other Neurodegenerative Movement Disorders, Clinical and Experimental Research, Department of Neurology, Hospital Clínic of Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer, University of Barcelona, Barcelona, Spain
  5. 5Movement Disorders Unit, Neurology Department, Hospital de la Santa Creu i Sant Pau, Barcelona, Spain
  6. 6Biomedical Research Institute, Barcelona, Spain
  7. 7Instituto de Salud Carlos III, Madrid, Spain
  8. 8Faculty of Medicine, Autonomous University of Barcelona, Barcelona, Spain
  9. 9Health Sciences Department, Universitat Oberta de Catalunya (UOC), Barcelona, Spain

Abstract

Background Most cellular dysfunctions in HD are associated with alterations in gene expression, with transcriptional dysregulation being a prominent feature of the disease. One of the mechanisms involved in this deregulation is the aberrant expression of microRNAs (miRNAs). Aberrant expression profiles of miRNAs have been identified as biomarkers in many different diseases. So far, several studies in the context of neurodegenerative disorders have focused on the identification and clinical application of miRNAs as biomarkers. However, the study of miRNAs in HD need to be further explored in particular related to the progression of the disease.

Aims In this study we aim to explore and validate miRNA expression profiles in fibroblasts of HD patients at pre-symptomatic and symptomatic stages.

Methods/techniques In a discovery phase we have assessed comprehensive genome-wide miRNA expression analysis of fibroblasts from pre-symptomatic, early symptomatic, middle/advanced symptomatic patients and controls. We have used the GeneChip miRNA 4.0 array from Affymetrix including probes for 2578 mature human miRNAs. In a validation phase we validated candidate miRNAs differentially expressed using the Taman Advance qPCR.

Results/outcome Our investigations have revealed specific signatures of miRNAs expression in HD patients along the disease progression with a downregulation of miRNAs at pre-symptomatic stages and an upregulation of miRNAs at symptomatic stages. Furthermore, we identified some mRNAs as biomarkers of disease progression that might identify when a patient become symptomatic such as, miR6124, miR210 and miR493, or when patients progress to a middle or advance stage of the disease, such as miR127.

Conclusions Our results indicate that miRNA alterations precede the onset of motor symptoms and highlight the potential of miRNA panels from fibroblasts as biomarkers for Huntington´s disease progression.

  • miRNAs
  • biomarkers
  • symptomatic stages
  • presymtpmatic stages
  • fibroblasts

Statistics from Altmetric.com

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.