Article Text

other Versions

Humoral and cellular immune responses to myelin protein peptides in Chronic Inflammatory Demyelinating Polyradiculoneuropathy
  1. Lara Sanvito (lara.sanvito{at}
  1. University of Nottingham, Division of Clinical Neurology, United Kingdom
    1. Anna Makowska (popywhite60{at}
    1. King's College London, United Kingdom
      1. Mohamed Mahdi-Rogers (mohamed.mahdi-rogers{at}
      1. King's College London, United Kingdom
        1. Robert DM Hadden (robert.hadden{at}
        1. King's College London, United Kingdom
          1. Mark Peakman (mark.peakman{at}
          1. King's College London, United Kingdom
            1. Norman Gregson (ngregson{at}
            1. King's College London, United Kingdom
              1. Raffaello Nemni (raffaello.nemni{at}
              1. Don C. Gnocchi Foundation, Italy
                1. Richard AC Hughes ({at}
                1. Department of Clinical Neurosciences, United Kingdom


                  Objectives: We sought evidence that chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) is an autoimmune disease by studying cellular and humoral immune responses to peripheral nerve myelin proteins.

                  Methods: We studied 40 CIDP, 36 healthy control subjects (HC) and subjects with non-immune mediated neuropathies (other neuropathies, ON) for antibodies by ELISA and cellular responses by cytokine ELISPOT (INF-γ, IL-10) and ELISA (IL-17) to synthetic peptides representing P0, P2 and PMP22.

                  Results: Antibodies to P0, P2 or PMP22 peptides were detected in only a minority of CIDP, both not treated (nT-CIDP) and treated (T-CIDP). IgG antibodies to P280-105 were significantly more frequent in CIDP than in HC (4/30 versus 0/32; p<0.05) but the difference from ON (1/25) was not significant. In ELISPOT assays, IFNγ was detected at low frequency in CIDP and did not differ from HC or ON. In contrast, IL-10 responses against P21-85 were more frequent in nT and T-CIDP (7/24 and 3/16) than HC (0/36; p<0.001 and p<0.05, respectively). The production of IL-17 in cell culture supernatants was not increased.

                  Conclusions: Antibodies to non-conformational antigenic epitopes of myelin proteins rarely occur in CIDP. None of the myelin protein peptides elicited IFN-γ responses but P2 elicited IL-10 responses significantly more often in CIDP patients than in controls. This reactivity may be part of an antigen specific Th2 type pathogenetic or regulatory mechanism or represent a transitory epiphenomenon due to nerve damage. In our study P2 was the protein antigen most likely to be involved in the aberrant immune responses in CIDP.

                  Statistics from

                  Request Permissions

                  If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.