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Antiganglioside antibodies in Guillain-Barré syndrome associated with SARS-CoV-2 infection
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  1. Carlo Civardi,
  2. Alessandra Collini,
  3. Dinha Joan Geda,
  4. Claudio Geda
  1. Department of Neurology, ASL TO4, Ivrea, Piedmont, Italy
  1. Correspondence to Dr Carlo Civardi, Department of Neurology, ASL To4, Ivrea, Piedmont, Italy; c_civardi{at}yahoo.com

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The pandemic SARS-CoV-2 is dramatically spreading around the world. Patients with COVID-19 typically present with viral pneumonia and resultant life-threatening respiratory complications. Although little information is available regarding the neurological manifestations of COVID-19, there are a few reports that describe Guillain-Barré syndrome (GBS) as an acute presentation of SARS-CoV-2.1 2 Here, we report a new case of COVID-19 initially presenting with acute GBS. On 24 March 2020, a 72-year-old woman arrived at the emergency department of our hospital presenting with lumbar pain, lower limb weakness and paresthesia, which started abruptly and progressed within 2 days (table 1). In the previous 10 days she had fever (Tmax 38.5°C, 101.3°F), anosmia, hypogeusia, dry cough and sore throat lasting for 3 days. Neurological examination disclosed symmetric weakness Medical Research Council (MRC) grade 3/5 in both legs and feet and MRC grade 4/5 in both arms and hands, with generalised areflexia. Laboratory results on admission were unremarkable except for an increase of fibrinogen (634 mg/dL; normal 200–400 mg/dL) and C reactive protein test (2.94 mg/dL; normal 0.1–0.75 mg/dL). Due to the pandemic situation, RT-PCR for nasopharyngeal swab SARS-CoV-2 was performed routinely and it displayed positivity. Nerve conduction studies (day 2) showed prolonged distal latencies, absent F waves, conduction blocks for the tibial nerves at the popliteal fossa and spare of sural nerve, supporting demyelinating neuropathy (online supplementary table e-1). Cerebrospinal fluid (CSF) testing showed a slight increase of cell counts (0.02 x109/L, normal 0-0.01x109 /L all monocytes), and a significant increased protein level (198 mg/dL, normal: <55 mg/dL), normal glucose level (65% of blood glucose level); RT-PCR assay for SARS-CoV-2 on CSF was negative. Screening tests for Epstein–Barr virus, Campylobacter jejuni, cytomegalovirus, herpes simplex virus, varicella zoster virus, influenza A virus, Haemophilus influenzae, HIV and culture examinations were negative. The findings were generally consistent with GBS.3 The antiganglioside antibodies ELISA test (Bühlmann GanglioCombi) was positive for IgG, anti-GM1, anti-GD1a and anti-GD1b. On this day, an intravenous immunoglobulin standard protocol was started. On the third day, after admission, muscle weakness rapidly evolved to a flaccid areflexic tetraparesis and facial diplegia. She developed respiratory failure with neuromuscular features and for this reason was admitted to the intensive care unit (ICU). During her ICU stay, she was also treated with hydroxychloroquine for 15 days and doxycycline hyclate. At day 25, she was negative to RT-PCR for nasopharyngeal swab SARS-CoV-2. At this point, although still partially respiratorily supported, we observed a significant clinical improvement: MRC superior limb 4/5 and 3/4 inferior limb.

Supplemental material

Table 1

Patientcharacteristics

Until now, six other cases of SARS-CoV-2 infection associated with GBS have been reported.1 2 As in other patients,1 ours showed a classic postinfectious profile as reported in GBS, while until now antiganglioside antibody detection for different gangliosides (anti-GM1, anti-GD1a and anti-GD1b) represents a novelty in GBS associated with SARS-CoV-2 infection. Antiganglioside autoantibodies of the IgG type are detected in a half of patients with GBS and their detection strongly supports the diagnosis.4 Antiganglioside antibodies are strongly associated with acute motor axonal neuropathy subtype more than acute inflammatory demyelinating polyneuropathy.4 Complexes of gangliosides may also be targeted by autoantibodies in GBS and its variants, the GD1a/GD1b is one of the more frequent especially when cranial nerves are involved.4 These results suggest a broad antiganglioside antibody response as observed in Zika virus-associated patients with GBS.5 Antiganglioside antibodies could be responsible for acute ascendent polyneuropathy but could also explain other neurological symptoms such as anosmia, as GD1a gangliosides are strongly expressed in the olfactory bulb.4 This new description gives new information about GBS as an acute presentation of SARS-CoV-2. Antiganglioside antibody evaluations could also be useful to understand other and more frequent neurological symptoms related to SARS-CoV-2.

References

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Footnotes

  • Contributors Review and critique of the manuscript.

  • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests None declared.

  • Patient consent for publication Obtained.

  • Provenance and peer review Not commissioned; externally peer reviewed.

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