Article Text

Original research
Characteristics and outcomes of COVID-19 associated stroke: a UK multicentre case-control study
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  1. Richard J Perry1,2,
  2. Craig J Smith3,4,
  3. Christine Roffe5,6,
  4. Robert Simister1,2,
  5. Saravanan Narayanamoorthi7,
  6. Richard Marigold8,
  7. Mark Willmot9,
  8. Anand Dixit10,
  9. Ahamad Hassan11,
  10. Terence J Quinn12,13,
  11. Sandeep Ankolekar14,
  12. Liqun Zhang15,
  13. Soma Banerjee16,
  14. Urwah Ahmed9,
  15. Nishita Padmanabhan6,
  16. Phillip Ferdinand6,
  17. Frances McGrane13,
  18. Azra Banaras1,
  19. Isobel H Marks16,
  20. David J Werring1,2
  21. on behalf of the SETICOS collaborators
    1. 1Comprehensive Stroke Service, Box 119, National Hospital for Neurology and Neurosurgery, London, UK
    2. 2Stroke Research Centre, UCL Queen Square Institute of Neurology, London, UK
    3. 3Manchester Centre for Clinical Neurosciences, Manchester Academic Health Science Centre, Salford, UK
    4. 4Division of Cardiovascular Sciences, Lydia Becker Institute of Immunology and Inflammation, University of Manchester, Manchester, UK
    5. 5Faculty of Medicine and Health Sciences, Keele University, Keele, Staffordshire, UK
    6. 6Stroke Service, University Hospitals of North Midlands NHS Trust, Stoke-on-Trent, UK
    7. 7Department of Stroke Medicine, Pennine Acute Hospitals NHS Trust, Greater Manchester, UK
    8. 8Department of Stroke Medicine, University Hospital Southampton NHS Foundation Trust, Southampton, UK
    9. 9Department of Neurology, University Hospitals Birmingham NHS Foundation Trust, Birmingham, UK
    10. 10Department of Stroke Medicine, Newcastle upon Tyne Hospitals NHS Foundation Trust, Newcastle upon Tyne, UK
    11. 11Department of Neurology, The Leeds Teaching Hospitals NHS Trust, Leeds, UK
    12. 12Institute of Cardiovascular and Medical Sciences, University of Glasgow, Glasgow, UK
    13. 13Stroke Service, Glasgow Royal Infirmary, Glasgow, UK
    14. 14Department of Neurology, King's College Hospital NHS Foundation Trust, London, UK
    15. 15Department of Neurology, St George's University Hospitals NHS Foundation Trust, London, UK
    16. 16Stroke Centre, Imperial College Healthcare NHS Trust, London, UK
    1. Correspondence to Dr Richard J Perry, Stroke Research Centre, UCL Queen Square Institute of Neurology, Queen Square, London WC1N 3BG, UK; richard.perry2{at}nhs.net

    Abstract

    Objective We set out to determine which characteristics and outcomes of stroke are associated with COVID-19.

    Methods This case-control study included patients admitted with stroke to 13 hospitals in England and Scotland between 9 March and 5 July 2020. We collected data on 86 strokes (81 ischaemic strokes and 5 intracerebral haemorrhages) in patients with evidence of COVID-19 at the time of stroke onset (cases). They were compared with 1384 strokes (1193 ischaemic strokes and 191 intracerebral haemorrhages) in patients admitted during the same time period who never had evidence of COVID-19 (controls). In addition, the whole group of stroke admissions, including another 37 patients who appeared to have developed COVID-19 after their stroke, were included in two logistic regression analyses examining which features were independently associated with COVID-19 status and with inpatient mortality.

    Results Cases with ischaemic stroke were more likely than ischaemic controls to occur in Asians (18.8% vs 6.7%, p<0.0002), were more likely to involve multiple large vessel occlusions (17.9% vs 8.1%, p<0.03), were more severe (median National Institutes of Health Stroke Scale score 8 vs 5, p<0.002), were associated with higher D-dimer levels (p<0.01) and were associated with more severe disability on discharge (median modified Rankin Scale score 4 vs 3, p<0.0001) and inpatient death (19.8% vs 6.9%, p<0.0001). Recurrence of stroke during the patient’s admission was rare in cases and controls (2.3% vs 1.0%, NS).

    Conclusions Our data suggest that COVID-19 may be an important modifier of the onset, characteristics and outcome of acute ischaemic stroke.

    This article is made freely available for use in accordance with BMJ’s website terms and conditions for the duration of the covid-19 pandemic or until otherwise determined by BMJ. You may use, download and print the article for any lawful, non-commercial purpose (including text and data mining) provided that all copyright notices and trade marks are retained.

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    Introduction

    COVID-19 is mainly recognised as a viral pneumonia, with a dry cough, high fever, shortness of breath and loss of taste and smell as its characteristic features.1 However, the virus responsible for this illness, SARS-CoV-2, may influence the presentation of ischaemic stroke,2 although this conclusion is controversial3 because of a lack of high quality evidence. In particular, the largest studies have compared patients with COVID-19 associated stroke with historical controls,4 5 whose strokes tend to be milder than those seen in contemporaneous controls,6 7 resulting in a bias towards overestimating the influence of COVID-19 on stroke severity and any other parameters correlated with severity.

    Our objectives were to determine whether COVID-19 is associated with: a different demographic group; a higher proportion of ischaemic strokes; higher D-dimer values7 in ischaemic strokes, as may be expected of a SARS-CoV-2 related hypercoagulable state8; a different distribution of stroke mechanisms, possibly with a predominance of large vessel occlusions (LVOs)9 10; more severe strokes with worse outcomes, including a higher inpatient mortality4 7; a higher rate of early recurrence of stroke; and a delay between the onset of symptoms of infection and of stroke.7 To address these objectives, we analysed data from a multicentre case-control study of strokes in patients admitted to 13 stroke services in England and Scotland during the 2020 COVID-19 pandemic.

    Methods

    Study design and participants

    SETICOS (Service Evaluation of The Impact of COVID-19 On Stroke) is an ongoing project in 13 stroke centres across England and Scotland (online supplemental table S1). Within this project, a case-control study was designed and reported according to STROBE guidelines.11 Approval was given by the clinical governance department in each hospital. The Health Research Authority confirmed that patient consent was not required for acquiring these surveillance data. There was a combination of retrospective and prospective collection. The study protocol was distributed to partner sites on 8 April 2020 and the first case report form was received on 14 April 2020. Estimated premorbid modified Rankin Scale (mRS) score and CT angiogram result were added to the list of fields on 29 April 2020, and our definition of recurrent stroke (see below) was clarified in data queries after data collection was complete.

    Supplemental material

    Anonymised case report forms were completed for patients admitted with a clinical diagnosis of stroke according to WHO criteria.12 Patients with subarachnoid haemorrhage were excluded. We anticipated that most centres would not be able to collect data on consecutive stroke admissions throughout the whole study period, so centres were asked to prioritise weeks during which patients with COVID-19 were admitted, and for any such week (Monday to Sunday) to include all strokes, regardless of SARS-CoV-2 status.

    Clinical data were extracted from discharge summaries or clinical notes, blood results were taken from electronic results systems and CT and MR angiogram data were obtained from radiology reports. Disability on discharge was determined using the mRS13 which varies from 0 (no symptoms) to 6 (death). For ischaemic strokes, the TOAST (trial of ORG 10172 in acute stroke treatment) classification14 was either taken from the discharge summary or was inferred from the clinical team’s documented assessment of likely stroke aetiology. TOAST categories ‘other determined aetiology’ and ‘undetermined aetiology’ were combined, as the choice between these two subgroups would depend on whether the clinician regarded COVID-19 as a ‘determined’ cause of stroke. Data were checked centrally for omissions and inconsistencies, and data queries were submitted to local centres until they were resolved.

    Defining cases (strokes in patients with SARS-CoV-2 at the time of stroke onset) and controls

    For our SARS-CoV-2 positive 'case' group, we included all strokes in patients who tested positive within 4 days of admission (or within 4 days of their stroke for inpatient strokes), even if they were negative on their first test, because reverse transcriptase-polymerase chain reaction (RT-PCR) on respiratory samples has a low sensitivity for SARS-CoV-2.15 These patients would be very unlikely to have acquired the infection in hospital and turned PCR positive within such a short time.16 We also included strokes in patients who already had clinical features suspicious of COVID-19 at the time of admission and were found to be SARS-CoV-2 positive at any point during the first 10 days of admission. The 'control' group consisted of patients who were either consistently SARS-CoV-2 negative or were never tested because they did not show symptoms or signs of COVID-19. Patients who were SARS-CoV-2 positive at some point during their admission but did not satisfy the criteria to be counted in the case group were excluded from the case-control study but were included in the logistic regression analyses.

    Definition of recurrent stroke

    Recurrent stroke was defined as any new stroke occurring with an onset separate from that of the index stroke. If a recurrent stroke occurred within 21 days of the index stroke, and was in the same vascular territory, then it was only included if it additionally fulfilled at least one of the following two criteria: (1) there was a new area of acute infarction on neuroimaging or (2) there was a new intracerebral haemorrhage anatomically separate from the infarct or haemorrhage of the index stroke.

    Statistical methods

    Sample size was determined pragmatically by the data collection that was feasible in each centre during the challenging circumstances of the COVID-19 pandemic, aiming for at least 500 patients in total and at least 80 cases, sufficient to allow for logistic regression analyses.17 Categorical variables were compared between cases and controls using the χ2 test, except for stroke recurrence rates where Fisher’s exact test was used because the expected rate of recurrent stroke in cases was fewer than five patients. D-dimer results were log10 transformed, resulting in distributions which approximated normal distributions, and then compared using the Student’s t test (for a difference in the means) and an F test (for a difference in the variances). Other continuous variables were compared using the Mann–Whitney U test.

    Binary logistic regression (Newton’s method), using demographics, vascular risk factors and stroke characteristics that differed between the cases and controls (threshold p<0.2), was used to establish which of these variables were independently associated with COVID-19 at onset. A further binary logistic regression analysis examined whether COVID-19 at onset was independently associated with inpatient mortality, using known predictors of early mortality18 as covariates of no interest. Where the admission National Institutes of Health Stroke Scale (NIHSS) score or estimated premorbid mRS score was not recorded, the median value for that covariate was entered in place of the missing data and a dummy covariate of no interest was added to the model, assigned a value of 1 for patients where the data point was missing and 0 for all other patients.

    Results

    Data included in the study

    We collected data from 1507 stroke admissions from the week commencing 9 March to the week commencing 29 June, from 13 stroke centres distributed across England and Scotland (online supplemental table S1). Early in the study period, patients were mainly tested for SARS-CoV-2 if there was clinical suspicion of COVID-19. Between the weeks commencing 9 March and 11 May, however, the proportion of asymptomatic patients tested increased progressively from 10.3% to 93.5%, and then remained at a mean of 95.3% for the rest of the study.

    Among the 123 strokes in patients who were SARS-CoV-2 positive at some point, 86 occurred in patients who had evidence of the infection at the time of stroke onset and were defined as cases (see methods). Nine of these (10.5%) had no clinical features of COVID-19 during their admission. One case was in a patient who had also been admitted with a previous control stroke (without COVID-19) earlier during the study period. Thirty-six patients with COVID-19 were excluded from the case-control comparisons because they appeared to have contracted the infection after their stroke (see online supplemental table S2 for their characteristics). One further case with intracerebral haemorrhage was excluded because the date of stroke onset could not be estimated. The control group consisted of 1384 strokes in 1377 patients. For 823 of these strokes, the patient was negative on PCR (59.5%) and in 561 strokes, the patient was not tested because they never had clinical evidence of COVID-19 (40.5%). All 1507 strokes were included in logistic regression analyses.

    Demographics and stroke characteristics

    Ethnicity was recorded in 86.0% of cases and in 77.7% of controls. The proportion of Asian patients among cases (17.6%) was more than twice that seen in the controls (7.3%, p<0.002, table 1), a disparity entirely attributable to the difference found in the ischaemic stroke group (18.8% vs 6.7%, p<0.0002, table 2). There was a correspondingly lower proportion of White patients among cases (table 1 and online supplemental figure S1). There was no significant difference in the proportion of Black patients between the two groups. Age and sex also did not differ between the two groups (online supplemental figure S2 and table 1).

    Table 1

    Stroke characteristics in cases (with evidence of COVID-19 at stroke onset) and controls (with no evidence of COVID-19 at any time), including ischaemic and haemorrhagic strokes

    Table 2

    Comparisons between cases and controls, shown separately for ischaemic stroke and intracerebral haemorrhage

    There was a higher proportion of ischaemic strokes in the cases (94.2%) compared with the controls (86.2%, p=0.03). Among ischaemic strokes that were classified according to TOAST criteria,14 there were no significant differences in the proportions of stroke attributed to large vessel atherosclerosis, cardioembolism or small vessel disease (table 2).

    D-dimers

    In patients with ischaemic stroke, D-dimers were measured in 23/81 (28.4%) cases and in 177/1193 (14.8%) controls; the distributions are shown on a log10 scale in figure 1. Treating the two distributions in figure 1 as normal distributions of potentially differing variance, the cases had a significantly higher mean log10 D-dimer (3.4) compared with controls (3.0, p<0.01), and also a higher variance (SD 0.83 vs 0.63, p<0.03), indicating a broader distribution of D-dimers in cases rather than a rightward shift of the whole curve.

    Figure 1

    Distribution of D-dimers (ng/mL) in cases and controls with ischaemic stroke, plotted on a log10 scale. Bin width of log10 D-dimer=0.25. The value shown on the x axis is the lower value of each bin. The value on the y axis is the percentage of D-dimer results falling within that range of values. Each arrow along the top row indicates one control who was anticoagulated for a deep vein thrombosis or pulmonary embolism during their admission; each arrow on the bottom row indicates a case who was anticoagulated for these indications.

    Timing of COVID-19 and stroke onset

    In the 44 cases with ischaemic stroke in whom both dates were recorded, the onset of the COVID-19 relevant symptoms of fever, cough or dyspnoea occurred a median of 6 days before stroke onset (shown as −6 in table 2). For the three cases with intracerebral haemorrhage who had both dates recorded, COVID-19 symptoms occurred a median of 4 days after stroke onset, and although the numbers are small, the difference between these two medians was significant (p<0.005, 2-tailed test).

    Stroke severity on admission

    Figure 2 shows the distributions of ischaemic stroke severities on admission for cases and controls with ischaemic stroke, measured using the NIHSS. Ischaemic strokes were significantly more severe in the cases (median NIHSS score=8) than in the controls (median NIHSS score=5, p<0.002). In patients with intracerebral haemorrhage, however, we did not find any significant difference in stroke severity between the two groups (median NIHSS scores=9 and 10, table 2).

    Figure 2

    Distribution of National Institutes of Health Stroke Scale (NIHSS) scores in cases and controls with ischaemic stroke. For each NIHSS range, the frequency of cases is shown as a percentage of the cases in which the NIHSS was measured, and similarly for controls. Bin width=2.

    Intracranial large vessel occlusion

    Among patients with ischaemic stroke, a CT or MR angiogram was available in 39/81 cases (48.1%) and in 627/1193 controls (52.6%). The proportion of patients with at least one intracranial LVO was not significantly different between cases and controls, whether expressed as a proportion of the patients who had this imaging done (41.0% vs 42.3%) or as a proportion of the whole stroke group (19.7% vs 22.2%). However, of the patients who had CT or MR angiography, the proportion of cases with multiple intracranial LVOs (17.9%) was more than twice that seen in controls (8.1%, p<0.03; figure 3).

    Figure 3

    Relationship between CT angiogram (CTA) or MR angiogram (MRA) findings and COVID-19 status in patients with ischaemic stroke. For cases and controls, shown are the proportions of those scanned in whom more than one intracranial large vessel occlusion (LVO) was reported, only one LVO was reported or no LVO was reported.

    Features independently associated with COVID-19 status

    We used binary logistic regression to assess which demographics, vascular risk factors and stroke characteristics recorded on admission were independently associated with COVID-19 at stroke onset. Asian ethnicity and multiple intracranial LVOs on CT or MR angiography were independently associated with COVID-19 at stroke onset, while premorbid mRS narrowly missed statistical significance (table 3). In a sensitivity analysis, the pattern of results was not significantly affected by omission of the 37 patients who developed COVID-19 but did not fulfil the criteria for cases.

    Table 3

    Binary logistic regression analysis to explore the association of demographics, vascular risks factors and stroke characteristics on admission with COVID-19 at the time of stroke. Covariates are shown in order of statistical significance

    Outcome from stroke

    Stroke recurrence during admission was very rare in cases (2.3%) and controls (1.0%), and with such small numbers it was not possible to demonstrate any significant difference between these groups, whether all strokes (table 1) or only ischaemic strokes (table 2) were included. The rates in cases and controls are in any case not directly comparable because cases had a longer median length of stay (table 1).

    The proportion of ischaemic strokes resulting in death during admission was significantly higher in cases (19.8%) than in controls (6.9%, p<0.00003, table 2), and the median mRS on discharge was higher in ischaemic cases (median mRS score=4) than in ischaemic controls (median mRS score=3, p<0.00003, figure 4). For intracerebral haemorrhages, we were unable to demonstrate any significant difference in disability or mortality between the 5 cases (median mRS score=4, mortality 20.0%) and the 191 controls (median mRS score=4, mortality 26.7%). COVID-19 at the onset of the stroke was independently associated with death during admission, even after correction for parameters known to be associated with early mortality18 (table 4).

    Table 4

    Binary logistic regression analysis demonstrating that COVID-19 at stroke onset was associated with inpatient mortality, even after correction for other clinical features known to be predictors of early mortality18

    Figure 4

    Distribution of modified Rankin Scale (mRS) scores on discharge in cases and controls with ischaemic stroke. The mRS score indicates the degree of disability: for example, 0=no symptoms, 1=symptoms without disability, 5=severe disability and 6=death during admission.

    Discussion

    This UK-based multicentre study provides a large case-control comparison, with contemporaneous controls, of strokes with and without COVID-19. Ischaemic strokes which were associated with COVID-19 at onset were: more likely to occur in Asian people; more likely to involve multiple LVOs; more severe; associated with higher D-dimer levels; and more likely to have a worse functional outcome or result in death. These findings add substantially to the previous smaller and methodologically limited studies, summarised in a recent systematic review,2 to confirm that COVID-19 has an important influence over the onset, characteristics and outcome of acute ischaemic stroke.

    Previous case series suggested that COVID-19 may be associated with a higher proportion of strokes with LVOs2 9 10 and this conclusion was confirmed in a single case-control study.19 In our study, by contrast, the overall incidence of at least one LVO was the same in the case and control groups. The proportion of cases with multiple LVOs, however, was more than twice that seen in the control group.

    LVO in COVID-19 may be a direct manifestation of a SARS-CoV-2 related hypercoagulable state, in which D-dimers are elevated.7 9 In our study, not only was the mean log10 D-dimer higher in cases than in controls, but also the variance of log10 D-dimers was higher in cases, suggesting greater heterogeneity in this group; one potential explanation would be the presence of a subgroup with higher D-dimers associated with a SARS-CoV-2 related coagulopathy.

    Our finding that ischaemic stroke associated with COVID-19 is more severe than in patients without COVID-19 is consistent with a small case-control study from New York, which reported more severe strokes in 32 patients with COVID-19 (median NIHSS score=19) than in 46 contemporaneous control patients without the infection (median NIHSS score=8).7 However, strokes in their contemporaneous control group were considerably more severe than they were in their historical controls (median NIHSS score=3). The difference presumably reflects a strong tendency for patients with minor stroke to stay away from hospital during the pandemic, or for doctors to avoid admitting them. Although we have observed the same effect in the UK,6 the degree of exclusion of minor strokes during the pandemic appears to have been less pronounced in our study (control group median NIHSS score=5). This disparity may reflect differences in public behaviour during the pandemic in different populations, or differences in admission strategies between the group of UK hospitals included in our study and the private healthcare organisation (NYU Langone Health) in the New York study.

    The shift towards greater stroke severity in COVID-19 negative patients during the pandemic6 means that registry studies comparing cases of COVID-19 associated stroke with historical controls4 5 are biased towards overestimating any influence of COVID-19 on stroke severity, and other correlated parameters, such as the incidence of LVOs and inpatient mortality rate, because historical controls have milder strokes than contemporaneous controls.7 We have avoided this bias by comparing our COVID-19 cases with contemporaneous controls. Even so, outcomes were still worse in cases than controls in our study, and in particular the inpatient mortality rate in our cases was significantly higher, a finding that remained after correction for other known risk factors for early inpatient mortality.18

    We found no evidence to support previous suggestions that SARS-CoV-2 infection may be associated with a younger age7 10 or male sex7 in stroke patients. In the case-control study from New York,7 by contrast, patients with COVID-19 associated strokes were younger (median 63 years) than controls (70 years). Most of their patients with COVID-19 associated stroke presented with COVID-19 as their primary diagnosis, so a likely explanation for this disparity is that, in their study, older patients with COVID-19 as their primary diagnosis may have been less likely to present and be admitted to hospital during the pandemic than younger patients.

    There was a median delay of 6 days from the onset of COVID-19 symptoms to the onset of ischaemic stroke, perhaps corresponding to the reported delay between COVID-19 onset and the development of a hypercoagulable state.8 However, COVID-19 does not appear to influence stroke solely through a single mechanism; no single aetiological category of ischaemic stroke seems to have been more strongly associated with COVID-19 infection than the others. We suggest that COVID-19 may provoke the onset of an ischaemic stroke through a variety of thrombotic and inflammatory mechanisms, promoting generation of thrombus in the heart20 or large vessels10 21 or via small vessel occlusion.22 Which of these mechanisms manifests in a given patient may be determined by that individual’s conventional vascular risk factors, such as atrial fibrillation, large vessel atheroma, hypertension or type 2 diabetes mellitus.

    Strengths of our study are that we included patients from 13 centres across the UK, so our results may be more representative of the overall stroke population than existing studies from a single hospital system or city. The use of contemporaneous controls allowed us to draw conclusions about the influence of COVID-19 on stroke severity, mortality and the incidence of LVO, inferences that cannot be made in studies comparing registry data with historical controls. In addition, the study was conducted according to a protocol agreed on early in the pandemic, before the frequency of cases in the UK had reached its peak.

    The main limitation of this study is that we were only able to report investigations done as part of routine clinical care. Some reports or tests were not available in all patients, which could introduce reporting or indication biases. For example, if there were a higher rate of reporting of ethnicity in cases compared with controls only in Asians, this reporting bias could in theory have exaggerated the association between Asian patients and COVID-19, although such a specific reporting bias seems implausible.

    D-dimers were measured in a higher proportion of cases (28.4%) than controls (14.8%), suggesting that the criteria for measuring them may have been narrower in the control group. Assuming that narrower test criteria select patients with higher D-dimers, there may have been a bias towards higher D-dimers in controls, resulting in an underestimate of the difference in D-dimers between the two groups. Although an indication bias could also have operated in the selection of patients for CT angiography, reassuringly we found no evidence of any bias towards performing this imaging in patients with COVID-19.

    Not all control patients were tested for SARS-CoV-2, so this group may have included patients with asymptomatic infection. In addition, we were reliant on SARS-CoV-2 RT-PCR results from respiratory swabs for COVID-19 diagnosis, and this test has a very poor sensitivity.16 However, the large size of our control group will have minimised the influence of false negative COVID-19 results over this group.

    If patients with minor stroke were more likely to attend if they had symptoms of COVID-19, then this effect could have reduced the median NIHSS score in cases, causing us to underestimate the difference in stroke severity between cases and controls. Finally, some COVID-19 associated strokes may have been missed in patients in whom clinical assessment was hampered by very severe COVID-19 infection.

    Our study provides the most compelling evidence yet that COVID-19 associated ischaemic strokes are more severe and more likely to result in severe disability or death, although the outlook is not quite as bleak as previous studies have suggested.7 8 Our results suggest the following recommendations for management of stroke patients during the ongoing COVID-19 pandemic.

    If at any point a stroke centre is not routinely testing all stroke admissions for SARS-CoV-2, patients presenting with ischaemic stroke and very elevated D-dimers with no other explanation should be considered for testing, even if the clinical suspicion of COVID-19 is otherwise low. Criteria for requesting CT angiography in stroke patients may in the future need to take account of their COVID-19 status, because the finding of multiple LVOs may mean that a specific management strategy is required, such as a different antithrombotic agent. On the other hand, in most patients with COVID-19 associated ischaemic stroke, very early anticoagulation is probably not warranted as a strategy to prevent inpatient stroke recurrence, as this outcome is too uncommon to justify the increased risk of secondary haemorrhage.

    Acknowledgments

    The authors would like to thank the whole team of SETICOS collaborators (listed in the supplemental material) who undertook their tasks largely on a voluntary basis during the exceptionally challenging time of the COVID-19 pandemic. We would also like to thank the two anonymous reviewers who, on behalf of this journal, gave us important detailed feedback on an earlier version of this manuscript. This work was undertaken at UCL Hospitals/UCL, which receives a proportion of funding from the Department of Health’s National Institute for Health Research (NIHR) Biomedical Research Centre's funding scheme.

    References

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    Supplementary materials

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    Footnotes

    • Twitter @cjsmithdr, @DrTerryQuinn, @issymarks

    • Contributors RJP, RS and DJW conceived the study. RJP wrote and distributed the protocol, designed the case report form and registration log, recruited centres to the study, received the case report forms and uploaded them into the database, performed statistical analyses and wrote the manuscript. RJP, CJS, CR, RS and DJW were the members of the Core Writing Group and critically reviewed the protocol, case report form and manuscript. CJS, CR, SN, RM, MW, AD, AH, TJQ, SA, LZ, AB and SB obtained approval for the study at their respective sites and took overall responsibility for the submission of accurate data. SN, RM, MW, TJQ, UA, NP, PF, FM, AB and IHM extracted data from clinical records and completed case report forms, working alongside collaborators listed in the supplemental materials. All authors critically reviewed the manuscript and approved the final version.

    • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

    • Competing interests CJS has received honoraria from Bayer, Sanofi and Pfizer, not related to the work presented here. DJW has received personal fees from Bayer, Alnylam and Portola, not related to the work presented here.

    • Patient consent for publication Not required.

    • Ethics approval Approval for the study was given by the clinical governance department in each hospital. The Health Research Authority confirmed that patient consent was not required for acquiring these surveillance data.

    • Provenance and peer review Not commissioned; externally peer reviewed.

    • Data availability statement Data are available on reasonable request, subject to restrictions imposed by patient confidentiality.

    • Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.

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