PT - JOURNAL ARTICLE AU - Cagli, S AU - Oktar, N AU - Dalbasti, T AU - Erensoy, S AU - Özdamar, N AU - Göksel, S AU - Sayiner, A AU - Bilgiç, A TI - Failure to detect <em>Chlamydia pneumoniae</em> DNA in cerebral aneursymal sac tissue with two different polymerase chain reaction methods AID - 10.1136/jnnp.74.6.756 DP - 2003 Jun 01 TA - Journal of Neurology, Neurosurgery &amp; Psychiatry PG - 756--759 VI - 74 IP - 6 4099 - http://jnnp.bmj.com/content/74/6/756.short 4100 - http://jnnp.bmj.com/content/74/6/756.full SO - J Neurol Neurosurg Psychiatry2003 Jun 01; 74 AB - Objective:Chlamydia pneumoniae (C pneumoniae) is a common cause of a usually mild, community acquired pneumonia. This organism, however, can spread from the respiratory tract into other parts of the body and has been detected in up to 70% of atheromatous lesions in blood vessels. Although the exact mechanism of the C Pneumoniae contribution to the pathogenesis of atherosclerosis remains unknown, prophylactic antibiotic trials are planned for people at high risk for coronary disease. Method: In this study the authors aimed to investigate C pneumoniae DNA content in the cerebral aneurysmal sac tissue with the aid of polymerase chain reaction (PCR) method. C pneumoniae DNA was searched in 15 surgically clipped and removed aneurysmal sac tissue and in two tumour (an ependymoma of the fourth ventricle and a craniofaringoma) samples by touchdown enzyme time release PCR (TETR PCR) targeting 16S rRNA gene and by nested PCR targeting ompA gene. Results: Both PCR methods were sensitive to detect in C pneumoniae 4×10−2 genomes. C pneumoniae DNA was not detected in any of the 17 sample tissues of these patients. Conclusion: The contribution of C pneumoniae in the development of intracranial aneurysms cannot be excluded despite the results of this study. Further studies on the possible role of C pneumoniae or any other micro-organisms in the pathogenesis of aneurysms should be performed.