TY - JOUR T1 - Failure to detect <em>Chlamydia pneumoniae</em> DNA in cerebral aneursymal sac tissue with two different polymerase chain reaction methods JF - Journal of Neurology, Neurosurgery &amp; Psychiatry JO - J Neurol Neurosurg Psychiatry SP - 756 LP - 759 DO - 10.1136/jnnp.74.6.756 VL - 74 IS - 6 AU - S Cagli AU - N Oktar AU - T Dalbasti AU - S Erensoy AU - N Özdamar AU - S Göksel AU - A Sayiner AU - A Bilgiç Y1 - 2003/06/01 UR - http://jnnp.bmj.com/content/74/6/756.abstract N2 - Objective:Chlamydia pneumoniae (C pneumoniae) is a common cause of a usually mild, community acquired pneumonia. This organism, however, can spread from the respiratory tract into other parts of the body and has been detected in up to 70% of atheromatous lesions in blood vessels. Although the exact mechanism of the C Pneumoniae contribution to the pathogenesis of atherosclerosis remains unknown, prophylactic antibiotic trials are planned for people at high risk for coronary disease. Method: In this study the authors aimed to investigate C pneumoniae DNA content in the cerebral aneurysmal sac tissue with the aid of polymerase chain reaction (PCR) method. C pneumoniae DNA was searched in 15 surgically clipped and removed aneurysmal sac tissue and in two tumour (an ependymoma of the fourth ventricle and a craniofaringoma) samples by touchdown enzyme time release PCR (TETR PCR) targeting 16S rRNA gene and by nested PCR targeting ompA gene. Results: Both PCR methods were sensitive to detect in C pneumoniae 4×10−2 genomes. C pneumoniae DNA was not detected in any of the 17 sample tissues of these patients. Conclusion: The contribution of C pneumoniae in the development of intracranial aneurysms cannot be excluded despite the results of this study. Further studies on the possible role of C pneumoniae or any other micro-organisms in the pathogenesis of aneurysms should be performed. ER -