PT  - JOURNAL ARTICLE
AU  - J D Colmenero
AU  - M I Queipo-Ortuño
AU  - J M Reguera
AU  - G Baeza
AU  - J A Salazar
AU  - P Morata
TI  - Real time polymerase chain reaction: a new powerful tool for the diagnosis of neurobrucellosis
AID  - 10.1136/jnnp.2004.049411
DP  - 2005 Jul 01
TA  - Journal of Neurology, Neurosurgery & Psychiatry
PG  - 1025--1027
VI  - 76
IP  - 7
4099  - http://jnnp.bmj.com/content/76/7/1025.short
4100  - http://jnnp.bmj.com/content/76/7/1025.full
SO  - J Neurol Neurosurg Psychiatry2005 Jul 01; 76
AB  - Background/methods: We compared the diagnostic yield of a real time polymerase chain reaction (PCR) assay in cerebrospinal fluid (CSF) samples with conventional microbiological techniques for the diagnosis of neurobrucellosis. Following amplification of a 223 bp sequence specific for Brucella genus, melting curve analysis was performed to verify the specificity of the PCR products. Results: All six patients with neurobrucellosis (three meningitis and three meningoencephalitis) had a positive real time PCR assay, whereas CSF cultures and Wright seroagglutination tests were positive in only two and four cases, respectively. Brucella specific amplicons were easily demonstrated by their characteristic melting temperature in all the real time PCR assays. Conclusion: LightCycler based real time PCR assay in CSF samples is more rapid and sensitive than conventional microbiological tests. This technique could be useful for the rapid diagnosis of neurobrucellosis.