Table 1

Test order

Test phase	Measures recorded	Time (min)
Baseline measures	Wt, ETCO₂, HR, BP, RPE, PF, MF, NM	5

Initial test trial:
Isometric tests:
Grip strength	MVC (kg)
50% MVC, 30 s	HR, BP, RPE, PF, MF, NM	5
Cognitive tests:
Digit span		5
Digit symbol		2
Verbal fluency		2
Telephone search		4
Lottery	HR, BP, RPE, PF, MF, NM	12

Treadmill test:
Set up:		5
Stage 1	ETCO₂, HR, BP, RPE, PF, MF, NM	3
Stage 2	HR, BP, RPE, PF, MF, NM	3
Stage 3	HR, BP, RPE, PF, MF, NM	3
Stage 4	HR, BP, RPE, PF, MF, NM	3
Stage 5^1-150	HR, BP, RPE, PF, MF, NM	3

Recovery	ETCO₂	2
		3

Repeat test trial:
Isometric tests:		5
Grip strength	MVC (kg)
50% MVC, 30 s	HR, BP, RPE, PF, MF, NM
Cognitive tests:
Digit span		5
Digit symbol		2
Verbal fluency		2
Telephone search		4
Lottery	HR, BP, RPE, PF, MF, NM	12
	Total time	90

↵1-150 Test ended at 85% age predicted maximum heart rate (85% HRmax); stages completed and time taken varied between subjects. HR= Heart rate; BP=blood pressure; RPE=rating of perceived exertion; PF= physical fatigue; MF=mental fatigue; NM=negative mood; MVC=maximal voluntary contraction; ETCO₂=end tidal CO₂.