Newly created mismatch primers for detecting Notch3 gene molecular variants
| Mismatch primers | Restriction enzyme | PCR Product sequence | Fragment size (post-digestion) |
| Exon 3, G291T, 200 bp | |||
| 5′-TGCCCACAG GTGCCCGCCTG A CT-3′ | BsrI | 5′-CTG ACTGGG↓TG-3′ | 177 bp |
| 5′-CTGACTGTGTG-3′ | |||
| Exon 4, C499T, 154 bp | |||
| 5′-AGGTGGGCAG GAGCAGAG AC AGC-3′ | Tth111 I | 5′-GGACG↓CTGTCTC-3′ | 131 bp |
| 5-GGATGCTGTCTC-3′ | |||
| Exon 4, G749A, 403p | |||
| 5′-TAGGGCTCAC TCACCAGG TC G-3′ | SalI | 5′-CCT G↓TCGACCTGG-3′ | 381 bp |
| 5′-CCTATCGACCTGG-3′ |
Italic letters=mismatched bases, Bold letters=mutation sites.
↓=restriction enzyme cutting sites, Underline=recognised sequences of restriction enzyme.