Table 2

Diagnostic array chart of the molecular variants of exons 3 and 4 of the Notch3 gene by restriction enzyme

Enzyme		Mutation/ polymorphism^2-150site		Exon	Digested fragments (bp)
Enzyme		Mutation/ polymorphism^2-150site		Exon	Wild type	Mutation/ polymorphism
BsaJ I	C346T	3	103, 60	163
	C406T	3	”	69
BsrI	G291T^2-153	3	177	200
	T631C	4	259, 161	420 (uncut)
Aci I	C381T^2-150	3	95, 71	166
	C474A^2-150	4	106, 60, 48	166^2-151
	C475T	4	”	166^2-151
	T514C	4	”	106, 48
	C583T	4	”	106, 87, 60^2-152
	C622T	4	”	106, 86, 60^2-152
Tth111 I	C499T^2-153	4	131	154
Hae III	C535T	4	118, 44	162
Nla III	G589T	4	221, 106	327
	A587G^2-150	4	”	327
NlaIV	T742G	4	80, 59, 61	80, 78, 59
Pst I	T712A	4	347, 73	420 (uncut)
Mwo I	C594A^2-150	4	107, 61	75
	G684A^2-150	4	”	168
SalI	G749A^2-153	4	381	403

↵2-150 Polymorphism.
↵2-151 Distinguished from each other by Nla IV (C474A) or MspA1 I (C475T) digestion.
↵2-152 Distinguished from each other by MspA1 I (C622T) digestion.
↵2-153 Mismatch primers.