Polymerase chain reaction primers used for the first and nested RT-PCR
| PC | Forward | Reverse |
|---|---|---|
| RT-PCR, reverse transcriptase polymerase chain reaction. | ||
| Ma1 first | 5′-AAATGGCGATGACACTG-3′ | 5′-GCTGCATTGCAGTCGTTCAC-3′ |
| Ma1 nested | 5′-AGTCTCCCAAACGGCCGCTC-3′ | 5′-GCTGCATTGCAGTCGTTCAC-3′ |
| Ma2 first | 5′-CCATGGCGCTGGCACTGTTAGA-3′ | 5′-CGGCCATAGCCATCTCGTTCC-3′ |
| Ma2 nested | 5′-CTGGACCTCACTGGGAATGG-3′ | 5′-CGGCCATAGCCATCTCGTTCC-3′ |
| Ma3 first | 5′-CCATGCCGTTGACCTTGTTA-3′ | 5′-GCAAATGTTGCCCTGTTCTC-3′ |
| Ma3 nested | 5′-CAGATACAGGGTGATTGGCA-3′ | 5′-GCAAATGTTGCCCTGTTCTC-3′ |